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A kind of bioactive polypeptide yfgsgfaapffivrhqllkk and its preparation method and application

A technology of biologically active peptides and biologically active peptides, applied in the field of protein, to achieve the effect of improving quality, improving ability, and good anti-inflammatory activity

Active Publication Date: 2022-03-18
ZHEJIANG PANDA DAIRY GRP COMPANY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the related functions of Cytochrome oxidase subunit 7C, mitochondrial protein polypeptide fragments in the prior art

Method used

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  • A kind of bioactive polypeptide yfgsgfaapffivrhqllkk and its preparation method and application
  • A kind of bioactive polypeptide yfgsgfaapffivrhqllkk and its preparation method and application
  • A kind of bioactive polypeptide yfgsgfaapffivrhqllkk and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Artificial Synthesis of Active Peptide YfgsgfaApffivrhqllkk

[0034] First, synthesis of bioactive peptide

[0035] Artificial synthetic bioactive peptide yfsgfaapffivrhqllkk.

[0036] Second, confirmation of bioactive peptide

[0037] 1) UPLC analysis

[0038] The UPLC conditions are as follows:

[0039] Instrument: Waters Acquity UPLC Ultra Eco Liquid Phase, Electric Spray, Four Lead, Flight Time Mass Spectrometer

[0040] Column Specifications: BEH C18 Column

[0041] Flow rate: 0.4ml / min

[0042] Temperature: 50 ° C

[0043] UV detection wavelength: 210nm

[0044] Number of injections: 2μL

[0045] Gradient conditions: A liquid: water containing 0.1% formic acid (V / V), B liquid: acetonitrile containing 0.1% formic acid (V / V)

[0046] Time (min)% a% B

0 95.0 5.0

1.50 80.0 20.0

3.50 60.0 40.0

5.00 40.0 60.0

7.00 15.0 85.0

8.00 0.0 100.0

11.00 0.0 100.0

11.50 95.0 5.0

13.00 95.0 5.0

[0047] 2) Mass spectrometry analysis

[0048] The mass spectrome...

Embodiment 2

[0062] Example 2 Immunoactive Experiment of Bioactive Peptide

[0063] I. Experimental Experiment of Biological Lymphocytes of YfgsgfaapffiVRHQLLKK (MTT Method)

[0064] 1. Experimental materials and instruments:

[0065] Reagents and materials: Experimental animal BALB / C mice (male 6-8 week old, Shanghai Jiaotong University Agriculture and Biology Animal Experiment Center); Example 1 obtained mouse spleen lymphocyte biologically active peptide yfsgfaapffiVRHQLLKKK; mouse lymph Cell extract (purchased from Visao Company); RPMI1640 medium (purchased from GIBCO); 3- (4,5-dimethylthiazole-2) -2,5-diphenyl tetrazol bromoric salt ( MTT, purchased from AMRESCO company); with knife bean protein (Cona, purchased from Sigma); bovine serum albumin (BSA, purchased from Genebase); gastric protease (purchased from Sigma); pancreatic enzyme (Corolase PP, purchase From AB Company).

[0066] Instrument: LRH-250F biochemical incubator, Shanghai Heng Technology Co., Ltd .; GL-22M High Speed ​​Fre...

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Abstract

The present invention relates to the field of proteins, in particular to a biologically active polypeptide YFGSGFAAPFFIVRHQLLKK and its preparation method and application. The amino acid sequence of the biologically active polypeptide YFGSGFAAPFFIVRHQLLKK is Tyr‑Phe‑Gly‑Ser‑Gly‑Phe‑Ala‑Ala‑Pro‑Phe‑Phe ‑Ile‑Val‑Arg‑His‑Gln‑Leu‑Leu‑Lys‑Lys. Through the in vitro immune function regulation experiment, it is verified that the polypeptide YFGSGFAAPFFIVRHQLLKK has a good immune regulation function. The biologically active peptide YFGSGFAAPFFIVRHQLLKK of the present invention can promote the increase in the induction of macrophage nitric oxide, improve the body's ability to resist external pathogen infection, reduce the incidence of body disease, significantly promote the proliferation of mouse lymphocytes, and improve the quality of life. Foods, health products and medicines with immune regulation function are of great significance.

Description

Technical field [0001] The present invention relates to the field of proteins, and more particularly to a biologically active polypeptide yfsgfaApffiVRHQLLKK and its preparation method and application. Background technique [0002] In recent years, bioactive peptides have become a word that people familiar with people. Because of its potential biological function, there is a more concerned that people have more and more attention, becoming one of the hotspots of scientific research. The beneficial effects of many bioactive peptides have also been proven well, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetes and other characteristics. More than 3,000 different biological active peptides have been reported in the BiopepEP-UMW in Biopep-UMW. [0003] At present, the study of bioactive peptides is concentrated on food-derived polypeptides, and there are few reports on non-food derived polypeptides and reports. It has been confirmed that...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02A61K8/66A61Q19/00A61K38/44A61P29/00A61P37/02A23L33/18
CPCC12N9/0053A61K8/66A61Q19/00A61P29/00A61P37/02C12Y109/03001A23L33/18A61K38/00A23V2002/00A23V2200/324
Inventor 张少辉郭婷婷栾媛媛李锡安占文静陈承余肖珊珊石毅梁世排梁世赞
Owner ZHEJIANG PANDA DAIRY GRP COMPANY