A method for regulating soybean nodulation and dense planting by gmpin1 gene mutation and its application

A gene mutation and soybean technology, applied in the field of plant genetic engineering, can solve the problem of few reports of major genes

Active Publication Date: 2022-05-24
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In addition, plant type is an important trait that determines the high yield of soybean. For decades, cultivating high-yielding soybeans with ideal plant type by optimizing soybean plant type has always been the goal of breeders and researchers. Genes are less reported [14]

Method used

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  • A method for regulating soybean nodulation and dense planting by gmpin1 gene mutation and its application
  • A method for regulating soybean nodulation and dense planting by gmpin1 gene mutation and its application
  • A method for regulating soybean nodulation and dense planting by gmpin1 gene mutation and its application

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Experimental program
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Effect test

Embodiment 1

[0045] Wild-type soybean (WT) and soybean triple mutant Gmpin1abc were bred in the following manner.

[0046] The light duration between soybean growth was 14 hours and the temperature was 25°C. Soybean seeds were germinated on vermiculite. On the 7th day after seed germination, the rhizobia BXYD3 with an OD value of 0.15 was evenly watered from the vermiculite surface to the soybean roots, followed by 1 / 10 nitrogen hydroponic solution for moisturizing and continued cultivation for 14 days.

[0047] The formula of 1 / 10 nitrogen hydroponic solution is shown in the following table:

[0048] Element KNO 3

[0049] The nodulation phenotypes of wild-type soybean (WT) and Gmpin1abc gene-edited soybean triple mutants are as follows figure 2 Gmpin1abc showed an overall decrease in nodule number and a decrease in nodule density (number of nodules divided by root area), but no significant change in the mean diameter of nodule nodule size profile. Number, density and di...

Embodiment 2

[0051] Wild-type soybean (WT) and soybean triple mutant Gmpin1abc were bred in the following manner.

[0052] The light duration between soybean growth was 14 hours and the temperature was 25°C. Soybean seeds were germinated on vermiculite. On the 10th day after the germination of the seeds, they were transferred into soil mixed with nutrient soil and loess and planted outdoors for 45 days.

[0053] Plant phenotypes of wild-type soybean (WT) and Gmpin1abc gene-edited soybean triple mutants are shown in the appendix image 3 As shown; the 10-day-old seedlings were transplanted into the soil and the overall plant type was short after 45 days of outdoor growth, and the angle of the lateral branches was reduced.

[0054] Lateral branch phenotypes of wild-type soybean and Gmpin1abc-L1 gene-edited soybean homozygous triple mutants Figure 4 shown; the branch angle of the homozygous triple mutant soybean Gmpin1abc-L1 without the CRISPR / Cas9 vector fragment was significantly reduce...

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Abstract

The invention discloses a method for regulating soybean nodulation and close planting by GmPIN1 gene mutation and its application. The application provided by the present invention is specifically the application of three homologous genes GmPIN1a, GmPIN1b, and GmPIN1c encoded by GmPIN1 in regulating soybean nodule formation and plant type establishment. Experiments of the present invention prove that soybean proteins GmPIN1a, GmPIN1b, GmPIN1c and their coding genes are closely related to the occurrence of soybean symbiotic nodulation, and after gene knockout, the number of soybean nodules is reduced and the side branch angle of the aerial part is significantly weakened. Moreover, plants without CRISPR / Cas9 carrier fragments were screened from the homozygous triple mutant progenies of transgenic soybean Gmpin1abc‑L1, providing excellent germplasm resources for regulating soybean symbiotic nitrogen fixation, dense planting, and high-yield breeding.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to a method for regulating soybean nodulation and dense planting by GmPIN1 gene mutation and its application. Background technique [0002] As one of the important economic crops in my country, soybean is rich in protein and oil and has very high nutritional value. However, with the continuous improvement of the living standards of our residents, my country's soybean production has been unable to meet its own needs, so that my country needs to import a large amount of soybeans from abroad every year. According to statistics, my country's soybean imports have rapidly increased from 13.94 million tons in 2001 to 88.06 million tons in 2018 [1] . Further analysis found that in the past 20 years, the yield per unit area of ​​soybeans in my country has hardly changed, and the yield per unit area of ​​soybeans in my country is much lower than that of the United States, Brazil and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H4/00A01H5/00A01H6/54
CPCC12N15/8218C12N15/8261C07K14/415A01H4/00
Inventor 陈栩崔原原柯美玉高震黄来妹陈娇梅
Owner FUJIAN AGRI & FORESTRY UNIV
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