Method for regulating soybean nodulation and close planting through GmPIN1 gene mutation and application of method

A gene mutation and soybean technology, applied in the field of plant genetic engineering, can solve the problem of few reports of major genes

Active Publication Date: 2021-03-16
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, plant type is an important trait that determines the high yield of soybean. For decades, cultivating high-yielding soybeans with ideal plant type by optimizing soybean plant type has always been the goal of breeders and researchers. Genes are less reported [14]

Method used

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  • Method for regulating soybean nodulation and close planting through GmPIN1 gene mutation and application of method
  • Method for regulating soybean nodulation and close planting through GmPIN1 gene mutation and application of method
  • Method for regulating soybean nodulation and close planting through GmPIN1 gene mutation and application of method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Wild-type soybean (WT) and soybean triple mutant Gmpin1abc were bred in the following manner.

[0046] The duration of light during soybean growth was 14 hours, and the temperature was 25°C. Soybean seeds were germinated on vermiculite. On the 7th day after seed germination, rhizobia BXYD3 with an OD value of 0.15 was evenly watered from the surface of the vermiculite to the soybean roots, followed by pouring 1 / 10 nitrogen hydroponic solution to keep it moist and continuing to cultivate for 14 days.

[0047] The formula of 1 / 10 nitrogen hydroponic solution is shown in the table below:

[0048] Element KNO 3

[0049] The nodulation phenotypes of wild-type soybean (WT) and Gmpin1abc gene-edited soybean triple mutants are shown in the appendix figure 2 As shown; Gmpin1abc showed that the nodule number decreased as a whole, and the nodule density (nodule number divided by root area) decreased, but the average diameter nodule size profile of nodules did not ...

Embodiment 2

[0051] Wild-type soybean (WT) and soybean triple mutant Gmpin1abc were bred in the following manner.

[0052] The duration of light during soybean growth was 14 hours, and the temperature was 25°C. Soybean seeds were germinated on vermiculite. On the 10th day after the seeds germinated, they were moved into the soil mixed with nutrient soil and loess and planted outdoors for 45 days.

[0053] The plant type phenotypes of wild-type soybean (WT) and Gmpin1abc gene-edited three mutants of soybean are shown in the appendix image 3 Shown; After 10 days of seedlings moved into the soil, the overall plant type was short after 45 days of outdoor growth, and the angle of side branches decreased.

[0054] The collateral phenotypes of wild-type soybean and Gmpin1abc-L1 gene-edited soybean homozygous triple mutant are shown in the appendix Figure 4 Shown; Compared with wild-type WT, the side branch angle (branch angle) of the homozygous triple mutant soybean Gmpin1abc-L1 without CRIS...

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Abstract

The invention discloses a method for regulating soybean nodulation and close planting through GmPIN1 gene mutation and application of the method. The application provided by the invention is specifically the application of three homologous genes GmPIN1a, GmPIN1b and GmPIN1c encoded by GmPIN1 in regulation and control of soybean root nodule formation and plant type establishment. Experiments of theinvention prove that the soybean proteins GmPIN1a, GmPIN1b and GmPIN1c and the encoding genes of the soybean proteins GmPIN1a, GmPIN1b and GmPIN1c are closely related to the occurrence of soybean symbiotic nodules, and after the genes are knocked out, the nodules number of soybeans is reduced, and the lateral branch included angle of the overground part is remarkably weakened. Moreover, plants without CRISPR / Cas9 carrier fragments are screened from the homozygous three mutant offspring of the transgenic soybean Gmpin1abcL1, and excellent germplasm resource reserves are provided for regulatingsoybean symbiotic nitrogen fixation, close planting and high-yield breeding.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to a method for regulating soybean nodulation and dense planting by GmPIN1 gene mutation and its application. Background technique [0002] As one of the important economic crops in our country, soybean is rich in protein and oil, and has very high nutritional value. However, with the continuous improvement of the living standards of our residents, the soybean production in our country can no longer meet our own needs, so that our country needs to import a large amount of soybeans from abroad every year. According to statistics, my country's soybean imports have rapidly increased from 13.94 million tons in 2001 to 88.06 million tons in 2018. [1] . Further analysis found that in the past 20 years, the yield per unit area of ​​soybeans in my country has hardly changed, and the yield per unit area of ​​soybeans in my country is far lower than that of the United States, Brazil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H4/00A01H5/00A01H6/54
CPCC12N15/8218C12N15/8261C07K14/415A01H4/00
Inventor 陈栩崔原原柯美玉高震黄来妹陈娇梅
Owner FUJIAN AGRI & FORESTRY UNIV
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