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Cytodiagnosis device and cytodiagnosis method
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A cell inspection and cell technology, applied in measurement devices, material inspection products, particle and sedimentation analysis, etc., can solve problems such as complicated operations, and achieve the effect of accurate observation, smooth and rapid inspection
Pending Publication Date: 2021-03-16
OLYMPUS CORP
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Problems solved by technology
In this inspection method, the operations in each process are performed at different places, so there is a problem that the operations become complicated
Method used
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no. 1 Embodiment approach
[0050] refer to Figure 1 to Figure 14 , the cell inspection device according to the first embodiment of the present invention will be described.
[0051] The cell inspection device 1 is a device for capturing and observing cells contained in a cell suspension. Such as figure 1 As shown, the cell inspection apparatus 1 includes a filter 10 , a chamber 20 , an injection unit 30 , a discharge unit 40 , a pressing member 50 , a light source 60 , and an observation unit 70 . The filter 10 , the chamber 20 and the pressing member 50 are disposed between the light source 60 and the observation unit 70 .
[0052] Such as figure 2 As shown, the filter 10 includes an annular frame body 11 and a filter unit 12 for capturing cells stuck on the surface of the frame body 11 . The filter unit 12 captures cells contained in the cell suspension, and thus has a smaller pore size than the cells. The filter material of the filter unit 12 is a membrane filter of a thin film, and a filter ha...
no. 2 Embodiment approach
[0098] use Figure 16 to Figure 18 A second embodiment of the present invention will be described.
[0099] The cell inspection device of this embodiment differs from the first embodiment in the configuration of the pressing member.
[0100] In the following description, the same reference numerals are assigned to the same components as those described above, and repeated descriptions will be omitted.
[0101] Such as Figure 16 As shown, an opening 25 is formed on an upper wall 20d of the chamber 20 . The pressing member 55 is provided at a position facing the observation portion 70 on the upper wall 20 d of the chamber 20 so as to close the opening 25 . The surface 55a (surface on the side of the internal space P) of the pressing member 55 facing the filter 10 is a flat surface. The pressing member 55 is made of a translucent material that transmits light from the light source 60 .
[0102] Similar to the first embodiment, the filter 10 is arranged in the internal space...
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Abstract
This cytodiagnosis method has: a preparation step for disposing, between a light source and an observation unit, a chamber having in the internal space thereof a filter for capturing cells; a first injection step for injecting a cell suspension from the front surface side of the filter into the internal space of the chamber; a first discharge step for discharging the cell suspension from the backsurface side of the filter to the outside of the chamber; a second injection step for injecting a staining solution for staining the cells from the front surface side of the filter into the internal space of the chamber; a second discharge step for discharging the staining solution from the back surface side of the filter to the outside of the chamber; a flattening step for flattening the filter by pressing the filter; and an observation step for observing the cells on the flattened filter by the observation unit through illumination from the light source.
Description
technical field [0001] The present invention relates to a cell inspection device and a cell inspection method. Background technique [0002] For diagnosing cancer and the like, biopsy is widely performed by collecting a part of tissue for pathological diagnosis. As minimally invasive biopsy, percutaneous needle aspiration biopsy, forceps / puncture needle biopsy using endoscope / ultrasonoscope (EBUS-GS, EBUS-TBNA, EUS-FNA) were performed, but due to Since the affected part is accessed indirectly through the ultrasound image, it may not be possible to reliably collect the target tissue. Then, rapid cytodiagnosis (Rapid on-site evaluation (Rapid on-site evaluation), hereinafter referred to as ROSE) of evaluating whether or not a part of collected samples are target samples on the spot was performed. In the usual ROSE, a part of the sample collected at the bedside is divided onto a glass slide by a pathologist or a cell examiner, and it is spread thinly on the glass slide by the...
Claims
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Application Information
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