A method and primers for identifying the litter performance of French Landrace pigs

A Landrace pig, performance technology, applied in the field of molecular breeding technology and primer pairs, can solve the problems of low heritability and achieve the effect of high estimated breeding value

Active Publication Date: 2022-04-22
TIANJIN AGRICULTURE COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Reproductive performance is a low heritability trait, which is controlled by micro-effect polygenes, so the molecular selection results of single mutation sites have certain limitations

Method used

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  • A method and primers for identifying the litter performance of French Landrace pigs
  • A method and primers for identifying the litter performance of French Landrace pigs
  • A method and primers for identifying the litter performance of French Landrace pigs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The pig samples, reproductive performance data and pedigree data come from French Large White pigs of Guangxi Shoumu Breeding Pig Breeding Co., Ltd.

[0056] 1. Pig FSHR 91773610 C>T site, MEP1A 41617218 G>T site and PLA2G4A 128109061 Determination of T>C mutation site.

[0057] (1) Collect pig ear samples and use DNA extraction kit to extract DNA from pig ear tissue.

[0058] (2) According to pig FSHR , MEP1A and PLA2G4A The gene GenBank Accession Number XM_021085881.1, XM_001928416 and XM_005667844, designed and synthesized primers as shown in Table 1:

[0059] Table 1 PCR amplification primers for mutation sites

[0060]

[0061] FSHR The primers are described in the Figures section.

[0062] (3) Using pig ear DNA as a template, using upper primers and PCR amplification system: 200 ng of genomic DNA, 5 µL of 10×PCR amplification buffer, 10 mM final concentration of dNTPs, 50 ng each of upper and lower primers, Taq DNA Polymerase 0.75mmol / L, make ...

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Abstract

The invention discloses a method and primers for identifying the litter performance of French-line Landrace pigs. The present invention also discloses FSHR 91773610 C>T, MEP1A 41617218 G>T, PLA2G4A 128109061 Primers for T>C amplification and methods for using the primers to detect, analyze and identify the litter performance of French Landrace pigs. The primer and method can realize early breeding of sows with high litter performance, increase the total litter size, the number of alive piglets and the number of healthy piglets, save feeding costs and shorten the breeding time. The method is simple to implement, easy to operate, and has high practical application value.

Description

technical field [0001] The invention relates to a molecular breeding technology and a pair of primers for single-gene single-site selection and multi-gene multi-site joint selection for reproductive performance of sows. Background technique [0002] The impact of African swine fever in 2018 caused severe damage to my country's pig industry, the number of reproductive sows decreased, and the price of pigs skyrocketed. Driven by huge profits, the national standard for selecting breeding sows has been lowered, and even commercial sows have been reserved for breeding. The germplasm resources have declined, and the reproductive performance of sows has seriously declined. The traditional breeding method for reproductive performance needs at least 1.2 years, which is a long time period. Molecular breeding related to reproductive performance is currently focused on the selection of single mutation sites. Reproductive performance is a low heritability trait, which is controlled by ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2565/627C12Q2535/122
Inventor 蒲蕾李若岚代宇星鲍恒郭亮
Owner TIANJIN AGRICULTURE COLLEGE
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