Method and primer for identifying farrowing performance of French landrace

A Landrace pig, performance technology, applied in the field of molecular breeding technology and primer pairs, can solve problems such as low heritability

Active Publication Date: 2021-03-19
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Reproductive performance is a low heritability trait, which is controlled by micro-effect polygenes, so the molecular selection results of single mutation sites have certain limitations

Method used

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  • Method and primer for identifying farrowing performance of French landrace
  • Method and primer for identifying farrowing performance of French landrace
  • Method and primer for identifying farrowing performance of French landrace

Examples

Experimental program
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Effect test

Embodiment 1

[0055] The pig samples, reproductive performance data and pedigree data come from French Large White pigs of Guangxi Shoumu Breeding Pig Breeding Co., Ltd.

[0056] 1. Pig FSHR 91773610 C>T site, MEP1A 41617218 G>T site and PLA2G4A 128109061 Determination of T>C mutation site.

[0057] (1) Collect pig ear samples and use DNA extraction kit to extract DNA from pig ear tissue.

[0058] (2) According to pig FSHR , MEP1A with PLA2G4A The gene GenBank Accession Number XM_021085881.1, XM_001928416 and XM_005667844, designed and synthesized primers as shown in Table 1:

[0059] Table 1 PCR amplification primers for mutation sites

[0060]

[0061] FSHR The primers are described in the Figures section.

[0062] (3) Using pig ear DNA as a template, using upper primers and PCR amplification system: 200 ng of genomic DNA, 5 µL of 10×PCR amplification buffer, 10 mM final concentration of dNTPs, 50 ng each of upper and lower primers, Taq DNA Polymerase 0.75mmol / L, make...

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Abstract

The invention discloses a method and primer for identifying farrowing performance of French landrace. The invention further discloses a primer for amplification of FSHR 91773610 C>T, MEP1A 41617218 G>T and PLA2G4A 128109061 T>C and a method for detecting, analyzing and identifying the farrowing performance of the French landrace by using the primer. According to the primer and the method, early breeding of sows with high farrowing performance can be realized, the number of the total farrowing, live farrowing and healthy farrowing of landrace is increased, the feeding cost is saved, and the breeding time is shortened. The method is simple to implement and convenient to operate, and has very high practical application value.

Description

technical field [0001] The invention relates to a molecular breeding technology and a pair of primers for single-gene single-site selection and multi-gene multi-site joint selection for reproductive performance of sows. Background technique [0002] The impact of African swine fever in 2018 caused severe damage to my country's pig industry, the number of reproductive sows decreased, and the price of pigs skyrocketed. Driven by huge profits, the national standard for selecting breeding sows has been lowered, and even commercial sows have been reserved for breeding. The germplasm resources have declined, and the reproductive performance of sows has seriously declined. The traditional breeding method for reproductive performance needs at least 1.2 years, which is a long time period. Molecular breeding related to reproductive performance is currently focused on the selection of single mutation sites. Reproductive performance is a low heritability trait, which is controlled by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2565/627C12Q2535/122
Inventor 蒲蕾李若岚代宇星鲍恒郭亮
Owner TIANJIN AGRICULTURE COLLEGE
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