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Casein rapid detection card and detection method thereof

A detection card and casein technology, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problem of false protein content, etc., and achieve the effect of fast detection, accurate results, and easy preparation

Inactive Publication Date: 2010-01-13
长沙安迪生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But if someone adds other substances containing nitrogen to the sample, the Kjeldahl method will be wrong, and false protein content will be obtained

Method used

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  • Casein rapid detection card and detection method thereof
  • Casein rapid detection card and detection method thereof
  • Casein rapid detection card and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A casein rapid test card, which is provided with a test strip 2 in the test card shell 1, the structure of the test card shell 1 and the setting of the test strip 2 refer to the attached figure 1 , The detection card shell 1 is a long flat thin shell shell, 70mm long, 20mm wide, 5mm thick, and the thin shell wall thickness is 1mm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0025] The test strip 2 is a thin strip with a multi-layer structure, the length of the strip is about 60mm, the width of the strip is about 4mm, and the thickness is about 2.5mm. The test strip 2 has a multi-layer structure: the bottom layer is a supporting backboard 5, which is a polyvinyl chloride plastic sheet with a thickness o...

Embodiment 2

[0028] The sample processing method is:

[0029] The processing method for the liquid milk sample is as follows: the liquid milk should be diluted 10 times with sample diluent (pH 7.6 10 mmol PBST) for detection.

[0030] The treatment method for the milk powder sample is: weigh 1 gram of milk powder sample, add 9mL sample diluent (pH 7.610mmol PBST), stir evenly in the test tube, and then use the sample diluent (pH 7.610mmol PBST) to carry out 10-fold dilution for use detection.

[0031] The treatment method for the milk product sample is: take 1g of the mashed milk product sample, add 9mL sample diluent (pH 7.610mmol PBST), stir evenly in the test tube, and then apply the sample diluent (pH 7.610mmol PBST) for 10 used for detection after dilution.

[0032] When using the casein rapid detection card for detection, the sample solution to be detected is first dripped from the sample injection hole 4 onto the sample pad 8 of the casein rapid detection card test strip 2, and th...

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Abstract

The invention relates to a casein rapid detection card and a detection method thereof, belonging to the technical field of dairy detection. A testing strip is arranged in a shell of the casein rapid detection card; a sample pad, an aurosol membrane, a pyroxylin membrane and a water absorption membrane are adhered on a support back plate in sequence; the aurosol membrane is a glass fibre membrane containing a casein-resistant antibody aurosol label, two testing tapes are arranged on the pyroxylin membrane, wherein one detection tape is a detection tape containing casein, and the other detection tape is a quality-control tape containing a rabbit-resistant antibody or mouse-resistant antibody. The invention has the advantages that the casein content of fluid milk, milk powder and dairy products can be directly detected by applying an immunological method. The casein rapid detection card is easy to prepare, convenient and rapid to use and accurate in results.

Description

technical field [0001] The invention relates to a casein rapid detection card and a detection method thereof, is a detection device for detecting casein, and belongs to the technical field of dairy product detection. Background technique [0002] Milk is an important food in people's daily life. Milk is rich in nutrition and is an important food for human beings. Its main components are: every 100 grams of milk contains 87 grams of water, 3.3 grams of protein, 4 grams of fat, 5 grams of carbohydrates, 120 mg of calcium, Phosphorus 93 mg, Iron 0.2 mg, Vitamin A 140 IU, Vitamin B 1 0.04 mg, Vitamin B 2 0.13 mg, 0.2 mg of niacin, 1 mg of vitamin C. Milk protein is the key nutrient in milk, and the protein content will be marked on the packaging of fresh milk. Under normal circumstances, the total protein content of milk is about 3.3%, of which about 2.5% is casein and 0.6% is whey protein. Casein is a kind of phosphoprotein, accounting for about 80% of bovine milk true prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/558G01N1/28
Inventor 周坚
Owner 长沙安迪生物科技有限公司
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