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Multiplexed sample plate

A sample plate and sample hole technology, which is applied in the method of sampling biological materials, containers and instruments used in laboratories, etc., can solve the problems of crosstalk between adjacent beads and easy crosstalk in porous arrangement, and achieve cost-effective effects.

Inactive Publication Date: 2021-03-19
DYNEX TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0024] Fourth, the porous arrangement disclosed in US Patent US-5620853 (Chiron Corporation) is particularly prone to crosstalk
[0029] A problem with known sample plates utilizing spherical reagent beads is that known arrangements suffer from crosstalk problems between adjacent beads when determining the luminosity of the reagent beads during the plate reading phase

Method used

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Examples

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Embodiment Construction

[0340] Regular sample plate

[0341] First refer to figure 1 Describe known arrangements. figure 1 A conventional sample plate comprising a plurality of sample wells 19 is shown. The sample plate may comprise, for example, a 9×6 array of sample wells 19 . For the sake of illustration, in figure 1 A single sample well 19 is shown in . A sample plate may comprise a strip of sample wells 19 , eg a sample plate may comprise a sample strip comprising eg a 1×9 or 1×6 array of sample wells 19 .

[0342] Each sample well 19 comprises a plurality of pockets, recesses or bores 21 provided in the bottom of the sample well 19 . exist figure 1 In the particular arrangement shown, the sample well 19 includes ten pockets, recesses or bores 21 formed or otherwise provided in the base of the sample well 19 .

[0343] Pockets, recesses or bores 21 may be provided along the edges or perimeter of sample well 19 and the center or central region of the base of sample well 19 may be sub...

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Abstract

A multiplexed sample plate comprising a sample well is disclosed. A plurality of substantially cylindrical reagent bead 2500 are inserted in use within a hole or aperture of the sample well. The substantially cylindrical reagent beads are positioned so as not to protrude beyond an upper surface of the base portion.

Description

[0001] Cross References to Related Applications [0002] none Background technique [0003] The present invention relates to a sample plate, a multiplex sample plate, a method for the determination of one or more analytes of interest, an automated device, a reagent for performing an enzyme-linked immunosorbent assay procedure Cartridge, a kit for performing a nucleic acid probe procedure, a method of manufacturing a sample plate, a method of manufacturing substantially or approximately cylindrical reagent beads, plugs or inserts, and a multiplex assay Methods. [0004] Disclosed are sample plates or multiplex sample plates that can be used to perform diagnostic tests such as enzyme-linked immunosorbent assay ("ELISA") procedures or other immunoassay procedures. Alternatively, testing against DNA or RNA sequences can be performed using sample plates or multiplex sample plates. [0005] Immunoassay procedures are the preferred way to test biological products. These procedur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00C12M1/32G01N33/543G01N35/00
CPCB01L3/50855B01L2300/0829B01L2300/0851B01L2200/025B01L2200/0668B01L2200/12B01L2200/141B01L2300/0609G01N33/54313B01J2219/00317B01J2219/00468B01J2219/00662G01N33/54366C12M33/00
Inventor 德瓦尔·A.·拉什卡里詹姆斯·S.·贝利
Owner DYNEX TECH
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