Applications of epicatechin in preparation of drugs for preventing or treating myocardial fibrosis
A technique for myocardial fibrosis and epicatechin, which is applied in the directions of drug combination, pharmaceutical formula, cardiovascular system diseases, etc., can solve the problem that the therapeutic effect of epicatechin on myocardial fibrosis has not been reported yet.
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Embodiment 1
[0049] In this example, a TAC mouse model was established, and the improvement effect of epicatechin (EPI) on mouse heart function was investigated by means of ultrasound imaging, histopathology, microscopic observation and collagen deposition.
[0050] The concrete experimental method of this embodiment is:
[0051] Mice were fed ad libitum diet for 7 days under normal room temperature and humidity conditions. Mice were anesthetized with tribromoethanol (20 mg / kg, MCE, USA). Then the mice were randomly divided into control group, aortic arch stenosis (TAC) group, epicatechin group and TAC+epicatechin group, epicatechin group and TAC+epicatechin treatment group were given EPI 1mg by intragastric administration / kg body weight · day, continuous gavage for 28 days.
[0052] (1) After TAC or 28 days of treatment, echocardiography (visualonic Vevo 1100, Canada) was used to monitor the cardiac function of the mice in each group, and the monitoring results were as follows: figure...
Embodiment 2
[0064] This example investigated whether EPI can protect cardiac fibroblasts (CFs) from AngII-induced transformation of cardiac fibroblasts through in vitro experiments.
[0065] In this embodiment, primary cultured neonatal rat cardiac fibroblasts are used as an in vitro myocardial fibrosis model, and the specific experimental methods are as follows:
[0066] Rat neonatal suckling mice (1-2d) under aseptic conditions, open the chest and take out the heart, rinse with D-Hanks solution 3 times and cut into about 1mm 3 Add 2ml of trypsin (1g / L) to digest the cells in a 37°C water bath for 2-3min, and transfer the trypsin solution containing the cells to DMEM medium containing 10% fetal bovine serum. Repeat the above operations until the color of the myocardial tissue block becomes transparent. According to the difference in the attachment speed between cardiomyocytes and fibroblasts, the method of differential attachment was used to distinguish and obtain myocardial fibroblasts...
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