Method for increasing seedling hardening survival rate of tissue culture seedlings of cremastra appendiculata

Abstract

The invention discloses a method for improving seedling hardening survival rate of tissue culture seedlings of cremastra appendiculata, and particularly relates to the technical field of planting. The method comprises the following steps: selecting mature cremastra appendiculata capsules, picking the cremastra appendiculata capsules, washing the cremastra appendiculatacapsules with running water for surface cleaning, and then placing the cremastra appendiculata capsules in an ultra-clean workbench. Corms at the base parts of buds of the cremastra appendiculata are used as explants, and adventitious buds are induced and rootless seedlings are subjected to rooting culture and seedling hardening, so that the time for culturing regeneration plants of the cremastra appendiculatais greatly shortened, a new way is provided for large-scale propagation of the regeneration plants of the cremastra appendiculata, the cremastra appendiculata cultivated in the mode grows well and is high in survival rate, the technical level in the prior art is remarkably improved, reliable technical guarantee is provided for large-scale and industrialized production of cremastra appendiculataseedlings, a new way is provided for large-scale propagation of the cremastra appendiculata, and the problem of shortage of current cremastra appendiculata plant resources and seedling resources is effectively solved.

Description

Technical field[0001]The present invention relates to the field of planting techniques, and more particularly, the present invention relates to a method of increasing a cuvettelm group cultivation.Background technique[0002]Du Du Lan also named Mao Chali mushrooms and abacus seven, for the wild rare drugs of Lanke Dufuran and ornamental plants, mainly distributed in East Asia, especially China's Guizhou and Sichuan, with fake scatters, taste, Gan, cold, there is a small toxic, clear heat detoxification, lungs and cough, blood circulation pain relief, swelling dextran, etc., internal anti-liver cancer, breast cancer and uterine cancer, etc., topical treatment of sore, snake insect bites, Skin burns or burns. Du Du Lan is not only a plant plant, but also a three-stage rare and endangered wild protective plant in my country.[0003]The natural storage of Duzhilan is very small, and the unplanned person is excavated. The wild resources are on the verge of disadvantage, Duzhi Lan is cold an...

AI Technical Summary

Problems solved by technology

[0003] The natural storage of Rhododendron orchids is very small, coupled with unplanned human excavation, the wild resources are on the verge of exhaustion. Rhododendron orchids like a cool and humid environment, and have strict requirements on the ecological environment. At present, they mainly rely on branch reproduction, and their reproduction coefficient is extremely low. In addition, because the seeds of rhododendron orchids are very small and have no endosperm, the success rate of seed reproduction in the natural environment is extremely low. However, the demand for rhododendron orchids in the medicinal material market is very large, causing people to carry out carpet-like digging, and wild resources are on the verge of Exhaustion has caused the price of medicinal materials to rise steadily. In order to save this rare species, ensure the sustainable utilization of its resources, and also to meet the market demand of this medicinal material, it is urgent to provide a method to improve the survival rate of Rhododendron orchid tissue culture hardening seedlings. method to solve the above problems