Method for increasing seedling hardening survival rate of tissue culture seedlings of cremastra appendiculata

A technology of rhododendron orchid group and survival rate, which is applied in horticultural methods, botanical equipment and methods, flower cultivation and other directions, can solve the problems of low natural storage of rhododendron orchid, rising prices of medicinal materials, low reproduction coefficient, etc., and achieves the solution of plant resources. And the effect of shortage of seedling resources, reliable technical support, and improvement of technical level

Pending Publication Date: 2021-04-16
武汉双螺旋生物科技有限公司
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The natural storage of Rhododendron orchids is very small, coupled with unplanned human excavation, the wild resources are on the verge of exhaustion. Rhododendron orchids like a cool and humid environment, and have strict requirements on the ecological environment. At present, they mainly rely on branch reproduction, and their reproduction coefficient is extremely low. In addition, because the seeds of rhododendron orchids are very small and have no endosperm, the success rate of seed reproduction in the natural environment is extremely low. How

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023]Method for increasing the cuvettecurization of fedica seedlings, including the following steps:

[0024]S1, select the ripened Duo-lace, pick a cuckoo, flush with flow water, then put it in the ultra-clean workbench, soak 2-3 min with 75% alcohol, and wash it with sterile water 3-5 times Then, then the plant disinfectant is soaked for 5 min, and finally was washed 3-5 times with sterile water, and the surface moisture was suction surface with anhydrous filter paper, and then the chip is taken away from the anatomical knife, and shake the Duran or the seed, and will shake out The Dufuran seeds first use 75% alcohol disinfection 15-20s, then wash 3-5 times with sterile water, and soak 8-12 min using a plant disinfectant, and finally washed 3-5 times of sterile water, and dry the surface with filtration. Moisture.

[0025]S2, then the resulting azodendron ornament is placed in a No. 1 medium consisting of 1 / 2-1 ms, 2.5-4.5 g / L agar and 0.1 mg / lnaa, induces culture under a tempera...

Embodiment 2

[0038]Method for increasing the cuvettecurization of fedica seedlings, including the following steps:

[0039]S1, select the ripened Duo-lace, pick a cuckoo, flush with flow water, then put it in the ultra-clean workbench, soak 2-3 min with 75% alcohol, and wash it with sterile water 3-5 times Then, then the plant disinfectant was soaked for 8 min, and finally was washed 3-5 times with sterile water, and the surface moisture was suction surface with anhydrous filter paper, and then the chip is taken away by the anatomical knife. The Dufuran seeds first use 75% alcohol disinfection 15-20s, then wash 3-5 times with sterile water, and soak 8-12 min using a plant disinfectant, and finally washed 3-5 times of sterile water, and dry the surface with filtration. Moisture.

[0040]S2, then the resulting azalea-landed seed was placed in a No. 1 medium consisting of 1 / 2-1 ms, 2.5-4.5 g / l agar and 0.1 mg / lnaa, induced in a temperature of 18 ° C, induced culture. 25-30 days, then transferred to...

Embodiment 3

[0053]Method for increasing the cuvettecurization of fedica seedlings, including the following steps:

[0054]S1, select the ripened Duo-lace, pick a cuckoo, flush with flow water, then put it in the ultra-clean workbench, soak 2-3 min with 75% alcohol, and wash it with sterile water 3-5 times Then, then the plant disinfectant is soaked for 10 min, and finally was washed 3-5 times with sterile water, and the surface moisture was suction surface with anhydrous filter paper, and then the chip is taken away by the anatomical knife. The Dufuran seeds first use 75% alcohol disinfection 15-20s, then wash 3-5 times with sterile water, and soak 8-12 min using a plant disinfectant, and finally washed 3-5 times of sterile water, and dry the surface with filtration. Moisture.

[0055]S2, then add the obtained azodendron ornament into No. 1 medium, 1 / 2-1 ms, 2.5-4.5 g / L agar and 0.1 mg / lnaa, induced culture under a medium having a temperature of 20 ° C. 25-30 days, then transferred to the second ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for improving seedling hardening survival rate of tissue culture seedlings of cremastra appendiculata, and particularly relates to the technical field of planting. The method comprises the following steps: selecting mature cremastra appendiculata capsules, picking the cremastra appendiculata capsules, washing the cremastra appendiculatacapsules with running water for surface cleaning, and then placing the cremastra appendiculata capsules in an ultra-clean workbench. Corms at the base parts of buds of the cremastra appendiculata are used as explants, and adventitious buds are induced and rootless seedlings are subjected to rooting culture and seedling hardening, so that the time for culturing regeneration plants of the cremastra appendiculatais greatly shortened, a new way is provided for large-scale propagation of the regeneration plants of the cremastra appendiculata, the cremastra appendiculata cultivated in the mode grows well and is high in survival rate, the technical level in the prior art is remarkably improved, reliable technical guarantee is provided for large-scale and industrialized production of cremastra appendiculataseedlings, a new way is provided for large-scale propagation of the cremastra appendiculata, and the problem of shortage of current cremastra appendiculata plant resources and seedling resources is effectively solved.

Description

Technical field[0001]The present invention relates to the field of planting techniques, and more particularly, the present invention relates to a method of increasing a cuvettelm group cultivation.Background technique[0002]Du Du Lan also named Mao Chali mushrooms and abacus seven, for the wild rare drugs of Lanke Dufuran and ornamental plants, mainly distributed in East Asia, especially China's Guizhou and Sichuan, with fake scatters, taste, Gan, cold, there is a small toxic, clear heat detoxification, lungs and cough, blood circulation pain relief, swelling dextran, etc., internal anti-liver cancer, breast cancer and uterine cancer, etc., topical treatment of sore, snake insect bites, Skin burns or burns. Du Du Lan is not only a plant plant, but also a three-stage rare and endangered wild protective plant in my country.[0003]The natural storage of Duzhilan is very small, and the unplanned person is excavated. The wild resources are on the verge of disadvantage, Duzhi Lan is cold an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00A01G22/63
Inventor 钱兴卓王娟
Owner 武汉双螺旋生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap