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A kind of technology of tributyrin and determination method, which is applied in the field of determination of tributyrin content
Inactive Publication Date: 2021-04-16
ANYOU BIOTECH GRP
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[0004] At present, there are no national standards, industry standards and other detection methods to determine the content of tributyrin
Method used
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Embodiment 1
[0050] Put 0.11491g of tributyrin standard product with a purity of 98% into a 100mL volumetric flask, fully dissolve it with n-hexane, dilute to the mark, and shake well to obtain a standard stock solution with a concentration of 1126.118μg / mL. Take 0.1mL, 0.3mL, 0.5mL, 0.7mL, 1mL standard stock solution respectively, add 0.9mL, 0.7mL, 0.5mL, 0.3mL to 0.1mL, 0.3mL, 0.5mL, 0.7mL, 1mL standard stock solution , 0mL n-hexane for dilution to obtain standard working solutions with concentrations of 112.612ug / mL, 337.835ug / mL, 563.059ug / mL, 788.283ug / mL and 1126.118ug / mL respectively.
[0051] The above-mentioned standard working solutions with different concentrations were injected into the gas chromatograph for testing. The chromatographic column was a wonda CAPWAX capillary column (30m×0.25mm×0.25um), the detector was an FID detector, and the programmed temperature was kept at 100°C for 2 minutes at the initial temperature. , and then the temperature was raised to 230°C at a rate...
Embodiment 2
[0056] The quality of tributyrin powder sample is 0.1324g, dissolves and constant volume with acetone, all the other conditions are the same as in Example 1. The peak area measured by the gas chromatograph was 278169, and 278169 was substituted into the standard curve equation to obtain the tributyrin concentration of the sample solution to be tested as 403.891 μg / mL. According to the content and concentration formula of tributyrin, the content of tributyrin is 30.505%.
[0057] The chromatogram of the tributyrin powder sample of embodiment 2 is as Figure 8 shown.
Embodiment 3
[0059] According to the method of Example 1, the same tributyrin powder sample was repeatedly tested, and the results are shown in Table 1.
[0060] Table 1 Repeatability test results (n=7)
[0061]
[0062] As can be seen from Table 1, the same tributyrin powder sample was repeatedly measured 7 times, RSD=1.71%, and the results showed that the method of the present invention had good repeatability.
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Abstract
The invention belongs to the technical field of substance content determination. The invention provides a method for determining the content of tributyrin, which comprises the following steps: mixing a tributyrin standard substance with an organic solvent to obtain a standard working solution; detecting the standard working solution by adopting a gas chromatograph, and obtaining a standard curve equation according to an external standard method; mixing a tributyrin powder sample with an organic solvent, and carrying out ultrasonic treatment and filtering treatment to obtain a to-be-detected sample solution; and detecting the to-be-detected sample solution by adopting a gas chromatograph, and obtaining the content of tributyrin in the to-be-detected sample solution according to the standard curve equation and the determined peak area. The determination method has the advantages of simplicity and convenience in operation, good repeatability, accuracy and the like, wherein the recovery rate of the tributyrin is between 98.8% and 100.2%.
Description
technical field [0001] The invention relates to the technical field of substance content determination, in particular to a method for determining tributyrin content. Background technique [0002] Studies have shown that tributyrin can regulate the intestinal structure and function of animals, repair intestinal mucosal damage, promote the growth of small intestinal villi, and achieve the effect of preventing digestive disorders and diarrhea in piglets after weaning. Tributyrin has the effect of improving feed digestibility, reducing the incidence of feces and watery feces in livestock and poultry breeding, and improving the weight gain of livestock and poultry and the utilization rate of feed. [0003] With the complete ban on antibiotics in the feed industry, tributyrin products are widely used in the feed industry due to their unique functionality. Tributyrin products fundamentally replace antibiotics in feed, eliminate antibiotic residues in feed, and produce high-quality...
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