The invention relates to a premix capable of promoting growth and reproduction of sows, as well as a preparation method and an application thereof. In parts by weight, per 1000 parts of the premix capable of promoting growth and reproduction of the sows comprises the following ingredients: 15-30 parts of selenium yeast, 150-200 parts of glycine chelated manganese, 36-50 part of copper lysine, 0.1-0.2 part of chromium picolinate, 1-2 parts of folic acid, 20-80 parts of linseed meal, 50-200 parts of glyceryl butyrate, and the balance carriers. The premix is added into basic daily feeds of the sows in a dosage that the addition amount of the premix is 0.5-1% of the total mass of the basic daily feeds of the sows. Compared with the prior art, the premix disclosed by the invention is capable of effectively improving food intake and milk yield of the sows, as well as satisfying nutrition needs so as to ensure nutrition, healthy development and production performance of the sows and the piglets. The premix promotes growth and breeding of the sows, and improves intestinal immune functions; moreover, the premix improves lactation performance, and reduces weight and back fat loss of the sows in the lactation period. In addition, the premix satisfies macro-element and trace-element needs of the sows in the breeding cycles, as well as improves absorption and utilization rates and reduces pollution to the environment.