Method for disinfecting and rapidly proliferating stems of aleurites montana

A stem segment and rapid technology, which is applied in the field of stem segment disinfection and rapid proliferation of Millennium tung, can solve the problems of difficult sterilization of Tung tung explants, insufficient regeneration plant technology, etc., and achieves shortened cultivation period, good induction effect, and seedling growth. exuberant effect

Active Publication Date: 2021-05-07
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
View PDF10 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The primary purpose of the present invention is to provide a disinfection method for the semi-lignified bud stem section of the Millennium tung tree in the current year, aiming at the problem that it is difficult to sterilize the explants of the Millennium tung tree and the technology of obtaining regenerated plants through tissue culture is insufficient

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for disinfecting and rapidly proliferating stems of aleurites montana
  • Method for disinfecting and rapidly proliferating stems of aleurites montana
  • Method for disinfecting and rapidly proliferating stems of aleurites montana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Do the following in order:

[0053] 1. Disinfection treatment of explants: On sunny mornings from April to June 2019, select young and tender Millennium paulownia stems with moderate thickness and no pests and diseases on the roof of the building, spray water and bring them back to the laboratory to collect the stems After the leaves are removed, wash them with an appropriate amount of washing powder for 1-2 times, then wash them with running water for at least 20 minutes, rinse them with sterile water, and place them in an ultra-clean workbench. Use 75% alcohol and 0.1% mercury chloride For disinfection, set different time gradients as shown in Table 1. During the disinfection process, use tweezers to stir constantly, rinse with sterile water 5-6 times, use sterile filter paper to blot the water on the surface of the stem section, cut off the browned part at both ends with a sterile blade, and inoculate the single-bud stem section into the culture medium. Statistical ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for disinfecting and rapidly proliferating stems of aleurites montana. The method comprises the processes of explant disinfection, axillary bud induction, rooting culture and the like. The method provided by the invention overcomes the problems of a high pollution rate and difficult culture of primary culture of the stems of the aleurites montana, has the advantages of the low pollution rate of explants, the high propagation rate and a short culture period, provides a way for rapid propagation of excellent aseptic seedlings of the aleurites montana, and provides possibility for industrialized seedling production of the aleurites montana and subsequent transgenic technology.

Description

technical field [0001] The invention belongs to the field of tissue culture of the Millennium tung tree, and in particular relates to a method for disinfecting and rapidly multiplying the stem section of the Millennium tree. Background technique [0002] Millennium tung (Vernicia montana) is a deciduous tree of Euphorbiaceae (Euphorbiaceae) genus Vernicia Lour. It is a typical south subtropical tree species. ~122°07′ are cultivated and distributed, and the main cultivation areas are Fujian, Guangdong, Guangxi, Jiangxi, Hunan and other provinces. Millennium tung tree has an oil content of 60% to 70%. One of the purposes of cultivation is to extract oil from seeds, which can be developed into biomass energy forests. It has good fast-growing and cold-resistant qualities, and has good development potential as a timber tree species, especially in areas where extreme low temperature is harmful, and its frost-resistant performance is obviously better than that of eucalyptus. Beca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001A01H4/00Y02P60/40
Inventor 李泽张慧马芳芳刘洋谭晓风曾艳玲张琳王珊杨昕悦高晓惠
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap