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Application of Sir2 family gene or protein in regulation and control of sizes of plant organs

A plant organ, gene technology, used in applications, plant products, genetic engineering, etc.

Active Publication Date: 2021-05-07
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Silence information regulator 2 (silence information regulator Sir2) protein is a family of NAD+-dependent protein deacetylases / ADP ribosyltransferases, and its targets are known to be involved in various cellular proteins of aging, DNA repair and metabolic regulation. But so far there is no report about Sir2 family proteins or genes in regulating plant organ size

Method used

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  • Application of Sir2 family gene or protein in regulation and control of sizes of plant organs
  • Application of Sir2 family gene or protein in regulation and control of sizes of plant organs
  • Application of Sir2 family gene or protein in regulation and control of sizes of plant organs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Cloning and sequence analysis of LcSRT1 and LcSRT2 genes

[0028] The present invention uses the previously obtained transcriptome data of Litsea cubeba (see accessionnumber SRA080286 in the SRA database of NCBI), and screens out two transcripts whose annotation information is Sir2 family protein, and both transcripts have completely open reads frame (ORF). The primers of two genes were designed according to the ORF sequence, and the Sir2 family genes were cloned from Litsea cubeba by RT-PCR. The Sir2 family genes are named as LcSRT1 and LcSRT2, which have 1,359bp (full CDS sequence as the nucleotide sequence shown in Seq ID No: 1) and 1,158bp (full CDS sequence as the nucleotide sequence shown in Seq ID No: 2) respectively. acid sequence), encoding 452 amino acids (amino acid sequence as shown in Seq ID No: 3) and 385 amino acids (amino acid sequence as shown in Seq ID No: 4), respectively.

[0029] Among them, the CDS amplification primer of LcSRT1 gene is...

Embodiment 2

[0035] Example 2 Overexpression of LcSRT1 and LcSRT2 genes can increase plant vegetative organs

[0036] The LcSRT1 and LcSRT2 genes obtained in Example 1 were heterologously expressed in tobacco (N. benthamiana), and the present invention found that in the respective positive transgenic plants, the expression levels of the two genes increased by 3,373% compared with the SRT gene in tobacco. times and 2,512 times, such as figure 1 shown. At the same time, the present invention also found that the transgenic positive plants of the two genes had a phenotype of enlarged plant organs compared with the wild type, which was reflected in the enlarged leaves, increased stem diameter (also called increased stem diameter), and increased stem diameter at the same developmental stage. A phenotype of increased stem height (also called increased length) and increased fresh weight of the plant.

[0037] Specifically: heterologous expression of LcSRT1 and LcSRT2 genes in tobacco. After pos...

Embodiment 3

[0039] Example 3 The overexpression of LcSRT1 and LcSRT2 genes can increase the production of plant monoterpenes

[0040] After the LcSRT1 and LcSRT2 genes obtained in Example 1 were heterologously expressed in tobacco (N. benthamiana), the present invention found that in the respective positive transgenic plants, the expression levels of the two genes were respectively increased compared with the SRT gene in tobacco. 3,373 times and 2,512 times, such as figure 1 shown. The present invention uses the headspace-SPME-GC-MS method to comparatively analyze the chemical components of the essential oils in LcSRT1ox, LcSRT2ox and wild-type plant WT, and specifically finds that (with figure 2 shown):

[0041] Compared with control wild-type plants, the overexpression of LcSRT1 and LcSRT2 genes can significantly increase the production of (1R)-(+)-α-pinene, D-limonene, cineole, β-ring citral and geranial. Metabolites are important constituents of monoterpenes in essential oils.

...

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Abstract

The invention discloses an application of a Sir2 family gene or protein in regulation and control of the sizes of plant organs, in particular to regulation and control for the sizes of leaves, regulation and control for the thicknesses of stems, regulation and control for the heights of plants and / or regulation and control for the fresh weight of the plants. The Sir2 family gene is an LcSRT1 gene or an LcSRT2 gene; and the application of the Sir2 family gene or protein is greatly expanded, and a new way is provided for the application of the Sir2 family gene or protein.

Description

technical field [0001] The invention relates to the field of plant molecular biology, in particular to the application of a Sir2 family gene or protein in regulating the size of plant organs. Background technique [0002] Silence information regulator 2 (silence information regulator Sir2) protein is a family of NAD+-dependent protein deacetylase / ADP ribosyltransferase, and its targets are known to be involved in various cellular proteins of aging, DNA repair and metabolic regulation. But so far there is no report about Sir2 family proteins or genes in the regulation of plant organ size. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a new application of Sir2 family gene or protein. [0004] In order to solve the above-mentioned technical problems, the present invention proceeds from Litsea cubeba (Lour.) Persoon, and carries out a series of related experiments. [0005] Litsea cubeba is a perennial deciduous ...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N9/10C12N15/82A01H5/12A01H5/04A01H5/00A01H6/82
CPCC12N9/1029C12N15/8243C12N15/8261Y02A40/146
Inventor 吴立文汪阳东陈益存高暝赵耘霄
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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