Culture medium for tissue culture of devirused potato and its prepn

A medium and potato technology, applied in the field of plant cultivation medium, can solve the problem of high cost of tissue culture seedlings and achieve the effect of reducing production costs

Inactive Publication Date: 2003-11-19
SHIJIAZHUANG INST OF AGRI MODERNIZATION CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the conventional culture method, the agar of the culture medium accounts for about 40% of the culture cost, which is one of the main reasons for the high cost of tissue culture seedlings

Method used

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Examples

Experimental program
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Embodiment Construction

[0009] The present invention will be further described below in conjunction with the example that virus-free potato tissue culture shoots produce:

[0010] During the preparation of the medium for potato tissue cultured seedlings, after preparing macronutrients, trace elements, iron salts, and organic components according to the composition of the medium, add 30 grams of sucrose and 60-70 grams of cornstarch to each liter of medium. Adjust the pH to 5.8, after heating and dissolving, divide into culture containers, seal, sterilize at 120°C, 1.1 atmosphere pressure for 20 minutes, and inoculate 10-15 potato tissue stems in each bottle after cooling. After sealing, cultivate in an environment with a temperature of 25±2°C, a light of 1500-2000 Lux, and a light time of 14-16 hours. After growing for 20-30 days, the differentiated seedlings were continued to be transferred to the above-mentioned cornstarch medium to carry out the cultivation of the next generation potato tissue cul...

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Abstract

This invention provides a virus-removed culture medium for group culture of potato and its composition includes (g / l): macro-elements: one or several of NH4NO3 1650 mg / l, KNO3 1900 mg / l, CaCl2.4H2O 440 mg / l, MgSO4.7H2O 370 mg / l and KH2PO4 170 mg / l; micro-elements: one on several of KI 0.38 mg / l, H2BO3 6.2 mg / l, MnSO4.4H2O 22.3 mg / l, ZnSO4.4H2O 8.6 mg / l, NaMoO4.2H2O 0.25 mg / l, CuSO4.5H2O 0.025 mg / l, CoCl2.6H2O 0.025 mg / l and FeSO4.7H2O 37.3 mg / l; complexing agent Na2.EDTA.2H2O 27.8 mg / l; organic componnets: one or several of inositol 100 mg / l, nicotinic acid 0.5 mg / l, pyridoxol hydrochloride 0.5 mg / l, thiamine hydrochloride 0.1 mg / l and glycine 2 mg / l; cane sugar 30 g / l, starch 60-70 g / l and water the rest. Use of the said culture medium can greatly lower the production cost of group culturing of potato seedling and make the quick factorized breeding of potato group cultured seedling become possible.

Description

technical field [0001] The invention relates to a culture medium for virus-free potato tissue culture and a preparation method thereof, which belongs to the technical field of plant culture medium, and is especially suitable for tissue culture and propagation of rhizome plants. Background technique [0002] Virus-free potatoes can effectively increase yield and improve quality, and virus-free seed potatoes are becoming more and more popular among potato growers. However, since the virus-free original seed potato must be obtained through tissue culture, and the tissue culture technology requires high investment, the production cost of the original original seed potato is too high. This causes the growers to be unable to bear the excessively high price of seed potatoes or the producers of the original original seed potatoes cannot maintain production due to the low profit, which leads to the difficulty in popularizing and applying the original original seedlings of the virus-f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01H4/00C12N5/04
Inventor 冯学赞安忠民王静
Owner SHIJIAZHUANG INST OF AGRI MODERNIZATION CHINESE ACAD OF SCI
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