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Breast cancer targeting molecular probe as well as preparation method and application thereof

A technology for targeting molecules and breast cancer, which can be applied in the biological field to solve problems such as lack of targeting

Pending Publication Date: 2021-05-28
厦门大学附属翔安医院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Targeting folate receptors on tumor cells using folate ligands, none of the above specifically targeted

Method used

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  • Breast cancer targeting molecular probe as well as preparation method and application thereof
  • Breast cancer targeting molecular probe as well as preparation method and application thereof
  • Breast cancer targeting molecular probe as well as preparation method and application thereof

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Embodiment

[0031] like figure 1 As shown, the present invention discloses a breast cancer targeting molecular probe, the probe is Lipo-ICG-CPP30, which includes liposome Lipo, the liposome is spherical, its interior is wrapped with indocyanine green ICG, and its exterior Modified with the polypeptide CPP30.

[0032] Liposomes include phospholipids, cholesterol, and distearoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE-PEG2000).

[0033] A preparation method for breast cancer targeting molecular probe Lipo-ICG-CPP30, the method comprising the following steps:

[0034] S1. Weigh 10 mg of CPP30 and dissolve it in 1.5 mL of phosphate buffered saline (PBS), then add 15 mg of phospholipid-polyethylene glycol-maleimide (DSPE-PEG-MAL), and react at room temperature for 12 hours to obtain a solution. The pH of PBS is 7.4;

[0035] S2, put a solution into a dialysis bag with a molecular weight cut-off of 3500, place it in a beaker equipped with deionized water and dialyze, remove un...

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PUM

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Abstract

The invention discloses a breast cancer targeting molecular probe and a preparation method and application thereof. The probe is Lipo-ICG-CPP30, the probe comprises a liposome Lipo, the liposome Lipo is spherical, the interior of the liposome Lipo is wrapped with indocyanine green ICG, and the exterior of the liposome Lipo is modified with polypeptide CPP30. The invention provides a breast cancer targeting molecular probe as well as a preparation method and application thereof. The probe has relatively high biological safety, CPP30 can target breast cancer cells, has relatively high membrane-penetrating capability, and can bring ICG into tumor cells, realize tumor cell localization and better guide visualization of tumors in an operation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a breast cancer targeting molecular probe and its preparation method and application. Background technique [0002] Breast cancer ranks first in the incidence and mortality of female malignant tumors in the world. Breast-conserving surgery is a standard operation for early breast cancer. It not only completely removes the tumor, but also preserves the breast and has a better cosmetic appearance. [0003] In vivo fluorescence imaging can not only be used to improve the diagnosis of tumor staging, monitor treatment response, but also detect recurrent or residual disease, and has been applied in the field of malignant tumor surgical navigation. With the development of fluorescent probes in the first near infrared window (NIR-I, 700-900nm) and in the second near infrared window (NIR-II, 1000-1700nm), fluorescence imaging technology has been The development of visible light imaging to n...

Claims

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Application Information

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IPC IPC(8): A61K49/00
CPCA61K49/0034A61K49/0056A61K49/0084
Inventor 张国君董媛媛杨瑞钦陈敏高艺洋
Owner 厦门大学附属翔安医院
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