Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gingival crevicular fluid markers for predicting oral periimplantitis and application thereof, and kit

An oral implant and kit technology, applied in the field of medicine and biology, can solve the problems of inability to reduce the occurrence of diseases and block the course of disease, lack of early warning and detection capabilities of diseases, etc., and achieve rapid detection, low cost and broad application prospects. Effect

Active Publication Date: 2021-05-28
BEIJING JISHUITAN HOSPITAL +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the clinical disease can be accurately diagnosed, the disease has entered the stage of clinical damage, lack of early warning and detection of the disease, unable to reduce the occurrence of the disease and block the course of the disease at an early stage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gingival crevicular fluid markers for predicting oral periimplantitis and application thereof, and kit
  • Gingival crevicular fluid markers for predicting oral periimplantitis and application thereof, and kit
  • Gingival crevicular fluid markers for predicting oral periimplantitis and application thereof, and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of liquid-phase chip kits for detection of IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, IL-8 biomarkers.

[0032] 1. Kit composition:

[0033] (1) 11-plex coated microspheres: containing encoded microspheres coated with IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, and IL-8 capture antibodies respectively;

[0034] (2) 11-plex biotin-labeled detection antibodies: IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, IL-8 detection antibodies labeled with biotin respectively;

[0035] (3) streptavidin phycoerythrin.

[0036] Among them, the clone numbers of IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, and IL-8 capture antibodies are ILA9-H18, ILB1-H67, eBio3A3-N2, 24612.111, 16F1, 48415, 6E7-C11-C9;

[0037] The clone numbers of IL-1α, IL-1β, IL-21, HGF, VEGF-A, and IL-8 detection antibodies are ILA8-H12, ILB1-H6, eBio2B2-G20, 0.N.326, P802B, 3IL8- H10, the detection antibody of GRO-α is a polyclonal antibody.

[0038] 2. The preparation method of the kit comprises the following ...

Embodiment 2

[0058] Application of IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, IL-8 liquid chip kit in predicting oral peri-implantitis.

[0059] 1. Purpose of the experiment:

[0060] It was proved that the concentrations of IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, and IL-8 in gingival crevicular fluid were higher in patients with peri-implantitis one week after implant restoration.

[0061] 2. Experimental object:

[0062] (1) On the premise of informed consent and meeting the inclusion conditions, select the enrolled cases and record personal basic information. The cohort of implant restoration patients came from 36 patients in Beijing Stomatological Hospital Affiliated to Capital Medical University.

[0063] (2) The gingival crevicular fluid was extracted from the patients 1 week after implant denture restoration, and the specimens were stored in a -80°C refrigerator.

[0064] 3. Reagent Preparation

[0065] Using the kit prepared in Example 1:

[0066] (1) Beads: Sonicate the requir...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a group of gingival crevicular fluid markers for predicting oral periimplantitis, application of the gingival crevicular fluid markers, a kit and a preparation method of the kit, and belongs to the technical field of biological medicines. The markers provided by the invention comprise one or more than two of IL-1alpha, IL-1beta, IL-21, HGF, VEGF-A, GRO-alpha and IL-8. The biomarker provided by the invention or the kit prepared from the biomarker is used for predicting oral periimplantitis, has the advantages of rapidness and accuracy in detection, low cost and the like, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the category of medical biology, and in particular relates to a gingival crevicular fluid marker for predicting oral peri-implantitis, a liquid phase chip kit for detection and a preparation method thereof. Background technique [0002] As an important treatment method for patients with missing teeth, implant restoration is more and more widely used in clinical practice. Prolonging the service life of implants is of great significance for ensuring the oral health of patients and improving the clinical effect of implant restoration. Studies have confirmed that implant restorations have many common features with natural tooth surrounding tissue structures, and there are also tissue structure differences, such as: collagen fiber content, fibroblast number, immune inflammatory cell components and number, capillary structure and number, etc. The difference is that the implant is closely integrated with the alveolar bone, and there ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543G01N33/531
CPCG01N33/6869G01N33/6863G01N33/6872G01N33/54313G01N33/531G01N2333/475G01N2333/545G01N2333/54G01N2333/5421G01N2333/52G01N2800/18
Inventor 李茵胡竹林王修芳宋瑾
Owner BEIJING JISHUITAN HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com