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Group of markers for predicting severe acute viral respiratory infectious diseases as well as application and kit thereof

A viral and respiratory technology, applied in the field of biomedicine, can solve problems such as inability to fully and accurately predict the severity and prognosis of patients

Active Publication Date: 2020-11-27
BEIJING DITAN HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, various current biomarkers have their limitations and cannot completely and accurately predict the severity and prognosis of patients

Method used

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  • Group of markers for predicting severe acute viral respiratory infectious diseases as well as application and kit thereof
  • Group of markers for predicting severe acute viral respiratory infectious diseases as well as application and kit thereof
  • Group of markers for predicting severe acute viral respiratory infectious diseases as well as application and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] In the present invention, the preparation method of the kit preferably includes the following steps:

[0033] (1) Coupling the capture antibodies coated with IL-8, IL-10, GITR, CD28, IL-18 respectively with the encoded microspheres to obtain IL-8, IL-10, GITR, CD28, IL- 18 capture antibody encoded microspheres;

[0034] (2) Link biotin to IL-8, IL-10, GITR, CD28, IL-18 detection antibodies respectively to obtain biotin-labeled IL-8, IL-10, GITR, CD28, IL-18 detection antibodies ;

[0035] There is no sequence relationship between the steps (1) and (2).

[0036] In the present invention, IL-8, IL-10, GITR, CD28, and IL-18 capture antibodies are coupled to encoded microspheres to obtain IL-8, IL-10, GITR, CD28, and IL-18 capture antibodies coated respectively. Encoded microspheres. In the present invention, the coupling method preferably includes the following steps:

[0037] a. Take the carboxyl microspheres and shake the microsphere suspension with a vortex oscilla...

Embodiment 1

[0061] Preparation of liquid-phase chip kits for detection of IL-8, IL-10, GITR, CD28, IL-18 biomarkers.

[0062] 1. Kit Composition

[0063] (1) 5-plex coated microspheres: containing encoded microspheres coated with IL-8, IL-10, GITR, CD28, and IL-18 capture antibodies respectively;

[0064] (2) 5-plex biotin-labeled detection antibodies: IL-8, IL-10, GITR, CD28, IL-18 detection antibodies labeled with biotin respectively;

[0065] (3) streptavidin phycoerythrin.

[0066]Among them, the clone numbers of capture antibodies IL-8, IL-10, GITR, CD28, and IL-18 are 3IL8-H10, JES3-9D7, 110416, 10F3, and H44, respectively; IL-8, IL-10, GITR, CD28, The clone numbers of IL-18 detection antibodies are I8-S2, BT-10, DT5D3, 37407, and W18160A, respectively.

[0067] 2. Kit Preparation

[0068] Include the following steps:

[0069] (1) The corresponding capture antibody is coated with the corresponding microsphere

[0070] a. Take carboxyl microspheres and vibrate the microsphere s...

Embodiment 2

[0088] Application of IL-8, IL-10, GITR, CD28, IL-18 liquid chip kits in the prediction of acute viral respiratory infectious diseases.

[0089] 1. Purpose of the experiment

[0090] It was proved that the baseline concentrations of IL-8, IL-10, GITR, CD28, and IL-18 at admission were high in severe and critically ill patients.

[0091] 2. Subjects

[0092] 1) On the premise of informed consent and meeting the inclusion conditions, select the enrolled cases and record personal basic information. The cohort of COVID-19 patients came from 83 COVID patients in Beijing Ditan Hospital Affiliated to Capital Medical University, including 6 asymptomatic patients, 53 mild patients, 14 severe patients, and 10 critical patients.

[0093] 2) Plasma was drawn from the patients before treatment when they were admitted to the hospital, and the samples were stored in a -80°C refrigerator.

[0094] 3. Reagent Preparation

[0095] The kit prepared in Example 1 was used.

[0096] (1) Beads:...

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Abstract

The invention provides a group of serological biomarkers for predicting severe acute viral respiratory infectious diseases as well as application and a kit of the serological biomarkers, and belongs to the technical field of biological medicines. The biomarkers disclosed by the invention are respectively IL-8, IL-10, GITR (Glutathione Isothiocyanate), CD28 and IL-18. The group of biomarkers including IL-8, IL-10, GITR, CD28 and IL-18 and the prepared kit are used for predicting the severe acute viral respiratory infectious diseases and have the advantages of rapid and accurate detection, lowcost and the like, and are wide in application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a group of markers for predicting the severity of acute viral respiratory infectious diseases and its application and kit. Background technique [0002] Severely ill patients with acute viral respiratory infectious diseases often suffer from severe hypoxemia, ARDS, shock, multiple organ dysfunction (MODS), combined or secondary bacterial infection and die. According to the different stages of the course of the disease and the changes in the condition, the emphases and specific measures of various treatment measures are also different. Improving the cure rate and reducing the fatality rate is one of the keys to the prevention and control of infectious diseases. Early identification of severe cases and timely treatment are the keys to reducing the fatality rate. Acute viral respiratory infectious diseases represented by novel coronavirus pneumonia have an acute on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/6893G01N33/54313G01N2800/26Y02A90/10
Inventor 孔雅娴曾辉张恒辉刘景院韩俊燕
Owner BEIJING DITAN HOSPITAL CAPITAL MEDICAL UNIV
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