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A set of gingival crevicular fluid markers for predicting oral peri-implantitis and their applications and kits

A group marker and kit technology, applied in the field of medicine and biology, can solve the problems of lack of early warning and detection of diseases, inability to reduce the occurrence of diseases and block the course of disease, and achieve the effects of rapid detection, low cost and broad application prospects.

Active Publication Date: 2022-07-01
BEIJING JISHUITAN HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the clinical disease can be accurately diagnosed, the disease has entered the stage of clinical damage, lack of early warning and detection of the disease, unable to reduce the occurrence of the disease and block the course of the disease at an early stage

Method used

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  • A set of gingival crevicular fluid markers for predicting oral peri-implantitis and their applications and kits
  • A set of gingival crevicular fluid markers for predicting oral peri-implantitis and their applications and kits
  • A set of gingival crevicular fluid markers for predicting oral peri-implantitis and their applications and kits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of liquid chip kits for IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, IL-8 biomarker detection.

[0032] 1. Kit composition:

[0033] (1) 7-plex coated microspheres: containing encoded microspheres coated with IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, and IL-8 capture antibodies;

[0034] (2) 7-plex biotin-labeled detection antibodies: IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, IL-8 detection antibodies labeled with biotin;

[0035] (3) Streptavidin phycoerythrin.

[0036] Among them, the clone numbers of IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, and IL-8 capture antibodies are ILA9-H18, ILB1-H67, eBio3A3-N2, 24612.111, 16F1, 48415, 6E7-C11-C9;

[0037] The clone numbers of IL-1α, IL-1β, IL-21, HGF, VEGF-A, and IL-8 detection antibodies are ILA8-H12, ILB1-H6, eBio2B2-G20, 0.N.326, P802B, 3IL8- The detection antibody of H10, GRO-α is a polyclonal antibody.

[0038]2. The preparation method of the kit includes the following steps:

[0039] (1) The corresponding c...

Embodiment 2

[0058] Application of IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, IL-8 liquid chip kits in predicting oral peri-implantitis.

[0059] 1. The purpose of the experiment:

[0060] It was proved that the concentrations of IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, and IL-8 in the gingival crevicular fluid one week after implant restoration were high in patients with peri-implantitis.

[0061] 2. Experiment subjects:

[0062] (1) On the premise of informed consent and meeting the inclusion conditions, selected cases were selected and basic personal information was recorded. The cohort of implant restoration patients came from 36 patients in Beijing Stomatological Hospital affiliated to Capital Medical University.

[0063] (2) The gingival crevicular fluid was extracted from the patients who underwent implant denture restoration at the time of admission, and the specimens were stored in a -80 ℃ refrigerator.

[0064] 3. Reagent Preparation

[0065] Using the kit prepared in Example 1...

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Abstract

The invention provides a group of gingival crevicular fluid markers for predicting oral peri-implantitis, an application thereof, a kit and a preparation method of the kit, which belong to the technical field of biomedicine. The markers described in the present invention include one or more of IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α and IL-8. The prediction of oral peri-implantitis by using the biomarker provided by the invention or the kit prepared by the biomarker has the advantages of rapid detection, accuracy, low cost and the like, and has broad application prospects.

Description

technical field [0001] The invention belongs to the category of medical biology, and in particular relates to a gingival crevicular fluid marker for predicting peri-implantitis in oral cavity, a liquid-phase chip kit for detection and a preparation method thereof. Background technique [0002] As an important treatment method for patients with missing teeth, implant restoration has become more and more widely used in clinical practice. Prolonging the service life of implants is of great significance for ensuring the oral health of patients and improving the clinical effect of implant restoration. Studies have confirmed that implant restorations have many common characteristics with the surrounding tissue structure of natural teeth, and there are also differences in tissue structure, such as: collagen fiber content, number of fibroblasts, composition and number of immune inflammatory cells, structure and number of capillaries, etc. Differences; the implant is closely combine...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/543G01N33/531
CPCG01N33/6869G01N33/6863G01N33/6872G01N33/54313G01N33/531G01N2333/475G01N2333/545G01N2333/54G01N2333/5421G01N2333/52G01N2800/18
Inventor 李茵胡竹林王修芳宋瑾
Owner BEIJING JISHUITAN HOSPITAL
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