A set of gingival crevicular fluid markers for predicting oral peri-implantitis and their applications and kits
A group marker and kit technology, applied in the field of medicine and biology, can solve the problems of lack of early warning and detection of diseases, inability to reduce the occurrence of diseases and block the course of disease, and achieve the effects of rapid detection, low cost and broad application prospects.
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Embodiment 1
[0031] Preparation of liquid chip kits for IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, IL-8 biomarker detection.
[0032] 1. Kit composition:
[0033] (1) 7-plex coated microspheres: containing encoded microspheres coated with IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, and IL-8 capture antibodies;
[0034] (2) 7-plex biotin-labeled detection antibodies: IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, IL-8 detection antibodies labeled with biotin;
[0035] (3) Streptavidin phycoerythrin.
[0036] Among them, the clone numbers of IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, and IL-8 capture antibodies are ILA9-H18, ILB1-H67, eBio3A3-N2, 24612.111, 16F1, 48415, 6E7-C11-C9;
[0037] The clone numbers of IL-1α, IL-1β, IL-21, HGF, VEGF-A, and IL-8 detection antibodies are ILA8-H12, ILB1-H6, eBio2B2-G20, 0.N.326, P802B, 3IL8- The detection antibody of H10, GRO-α is a polyclonal antibody.
[0038]2. The preparation method of the kit includes the following steps:
[0039] (1) The corresponding c...
Embodiment 2
[0058] Application of IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, IL-8 liquid chip kits in predicting oral peri-implantitis.
[0059] 1. The purpose of the experiment:
[0060] It was proved that the concentrations of IL-1α, IL-1β, IL-21, HGF, VEGF-A, GRO-α, and IL-8 in the gingival crevicular fluid one week after implant restoration were high in patients with peri-implantitis.
[0061] 2. Experiment subjects:
[0062] (1) On the premise of informed consent and meeting the inclusion conditions, selected cases were selected and basic personal information was recorded. The cohort of implant restoration patients came from 36 patients in Beijing Stomatological Hospital affiliated to Capital Medical University.
[0063] (2) The gingival crevicular fluid was extracted from the patients who underwent implant denture restoration at the time of admission, and the specimens were stored in a -80 ℃ refrigerator.
[0064] 3. Reagent Preparation
[0065] Using the kit prepared in Example 1...
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