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Application of alpha-lipomycin as marker for detecting and diagnosing hypertension to prepare corresponding detection tool

A detection tool, technology for high blood pressure, applied in the field of biomarkers

Inactive Publication Date: 2021-06-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] After investigation, there is no relevant literature report on the correlation between α-lipomycin and hypertension by scholars at home and abroad (from PUBMED, CNKI, VIP China Journal Network), so α-lipomycin is still in the research field of hypertension biomarkers. in blank stage

Method used

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  • Application of alpha-lipomycin as marker for detecting and diagnosing hypertension to prepare corresponding detection tool
  • Application of alpha-lipomycin as marker for detecting and diagnosing hypertension to prepare corresponding detection tool
  • Application of alpha-lipomycin as marker for detecting and diagnosing hypertension to prepare corresponding detection tool

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Determination of α-lipomycin levels in high-salt diet-induced hypertensive mice.

[0044] A total of 13 healthy male C57BL / 6J mice of 18-20 g were divided into two groups, namely the experimental group (n=7) and the blank group (n=6); wherein the experimental group was fed with 8% high-salt feed, The blank group was given a normal diet, and after five weeks of feeding, the blood pressure of the mice was measured by the tail cuff method, and the hypertensive mice induced by a high-salt diet were obtained in the experimental group.

[0045] Orbital blood collection collected 500 μL of non-anticoagulated blood from the hypertensive mice in the experimental group and the mice in the blank group, and kept it in the dark for 2 hours at room temperature, centrifuged at 3000 rpm for 10 minutes, and collected the separated serum; the collected serum was in a ratio of 1:3 Add pre-cooled anhydrous methanol (containing 0.1% BHT), vortex well and centrifuge at 14000g for 10min at 4°...

Embodiment 2

[0048] Determination of α-lipomycin levels in hypertensive subjects.

[0049] A total of 87 volunteers were recruited in this clinical trial, and the upper arm blood pressure of the volunteers was measured with an electronic sphygmomanometer. According to the measured blood pressure values, they were divided into two groups: non-hypertensive group (n=17) and hypertensive group (n=70). Volunteers whose blood pressure was continuously measured greater than 140 / 90 mm Hg in a quiet state were classified as hypertensive groups into the hypertensive group, and the rest of the healthy population was classified as the non-hypertensive group. All volunteers signed an informed consent form, and all volunteers met the inclusion and exclusion criteria. The standards are as follows:

[0050] Inclusion criteria: healthy people or clinically diagnosed hypertension.

[0051] Exclusion criteria: (1) known or suspected secondary hypertension; (2) suffering from severe chronic diseases (inclu...

Embodiment 3

[0055] Serum α-lipomycin content in hypertensive population is correlated with salt intake.

[0056] Clinically, the 24-hour urine sodium value is used as the "golden indicator" to reflect the patient's daily sodium chloride intake. We collected 24-hour urine samples from hypertensive patients (n=56) who had a regular diet, and used the ion electrode method to measure the urine sodium concentration; Pearson correlation analysis.

[0057] The result is as image 3 As shown, the content of α-lipomycin in the serum of the hypertensive population is correlated with the patient's daily salt intake (24-hour urine sodium), and the difference is significant (r=0.623, P<0.001).

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Abstract

The invention discloses application of alpha-lipomycin as a marker for detecting and diagnosing hypertension to prepare a corresponding detection tool. A detection object of the detection tool is a to-be-detected sample collected from a subject. The content of alpha-lipomycin in hypertension model mice and hypertension patients is relatively high, and the alpha-lipomycin can be used for establishing a detection method or preparing a detection and diagnosis kit and can be used for auxiliary diagnosis and risk assessment of hypertension and prehypertension.

Description

technical field [0001] The invention relates to the technical field of biomarkers, in particular to an application of using α-lipomycin as a marker for detecting and diagnosing hypertension to prepare corresponding detection tools. Background technique [0002] Cardiovascular disease remains the leading cause of harm to human health and death. There is a close causal relationship between the level of blood pressure and the risk of cardiovascular and cerebrovascular diseases and death. At present, the number of patients with cardiovascular diseases in China has exceeded 290 million, of which 245 million have high blood pressure. Although elevated blood pressure is the most important clinical manifestation of hypertension, it is not necessarily the only and ideal diagnostic criterion. During the occurrence and development of hypertension, various metabolic disorders are often associated. For example, metabolic abnormalities such as obesity, impaired glucose tolerance, and dy...

Claims

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Application Information

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IPC IPC(8): G01N30/72G01N30/02
CPCG01N30/72G01N30/02
Inventor 马鑫周婷婷王志玮刘靓靓肖望
Owner JIANGNAN UNIV
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