Rhizoma drynariae crude product and UPLC fingerprint spectrum construction and identification method thereof

A construction method and technology of fingerprints, which are applied in the field of detection and identification of processed traditional Chinese medicine, to achieve the effect of wide application range, comprehensive detection means, and good reproducibility

Active Publication Date: 2021-06-11
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Based on this, it is necessary to address the problem of limitations in the traditional technology, and the main purpose of the present invention is to provide a kind of rhizoma rhizome and its processed product UPLC fingerprint Construction and Identification Methods

Method used

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  • Rhizoma drynariae crude product and UPLC fingerprint spectrum construction and identification method thereof
  • Rhizoma drynariae crude product and UPLC fingerprint spectrum construction and identification method thereof
  • Rhizoma drynariae crude product and UPLC fingerprint spectrum construction and identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0120] Embodiment 1, the construction of UPLC fingerprint of bone dry tonic product

[0121] 1. Test method

[0122] 1.1 Preparation of reference solution

[0123] Precisely weigh the appropriate amount of 5-hydroxymethylfurfural reference substance, protocatechuic acid reference substance, new erionthidrin reference substance, and naringin reference substance, add methanol to make each 1mL contain 5-hydroxymethylfurfural 615.34μg, A solution of 280.12 μg of protocatechuic acid, 257.54 μg of eriodinin, and 297.67 μg of naringin was shaken to obtain a mixed reference solution.

[0124] 1.2 Chromatographic conditions

[0125] Octadecylsilane-bonded silica gel was used as filler, methanol was used as mobile phase A, and 0.05% phosphoric acid aqueous solution was used as mobile phase B to carry out gradient elution with a flow rate of 1.0 mL / min, column temperature of 30°C, and identification wavelength of : 0min~11min, 283nm; 11min~20min, 260nm; 20min~30min, 283nm; 30min~39min...

Embodiment 2

[0142] Embodiment 2, the construction of the UPLC fingerprint spectrum of dried drynariae sand scalding processed products

[0143] 1. Test method

[0144] 1.1 Preparation of reference solution

[0145] Precisely weigh the appropriate amount of 5-hydroxymethylfurfural reference substance, protocatechuic acid reference substance, new erionthidrin reference substance, and naringin reference substance, add methanol to make each 1mL contain 5-hydroxymethylfurfural 615.34μg, A solution of 280.12 μg of protocatechuic acid, 257.54 μg of eriodinin, and 297.67 μg of naringin was shaken to obtain a mixed reference solution.

[0146] 1.2 Chromatographic conditions

[0147] Octadecylsilane-bonded silica gel was used as filler, methanol was used as mobile phase A, and 0.05% phosphoric acid aqueous solution was used as mobile phase B to carry out gradient elution with a flow rate of 1.0 mL / min, column temperature of 30°C, and identification wavelength of : 0min~11min, 283nm; 11min~20min,...

Embodiment 3

[0165] Embodiment 3, the construction of drynaria rhizome microwave-processed product UPLC fingerprint

[0166] 1. Test method

[0167] 1.1 Preparation of reference solution

[0168] Precisely weigh the appropriate amount of 5-hydroxymethylfurfural reference substance, protocatechuic acid reference substance, new erionthidrin reference substance, and naringin reference substance, add methanol to make each 1mL contain 5-hydroxymethylfurfural 615.34μg, A solution of 280.12 μg of protocatechuic acid, 257.54 μg of eriodinin, and 297.67 μg of naringin was shaken to obtain a mixed reference solution.

[0169] 1.2 Chromatographic conditions

[0170] Octadecylsilane-bonded silica gel was used as filler, methanol was used as mobile phase A, and 0.05% phosphoric acid aqueous solution was used as mobile phase B to carry out gradient elution with a flow rate of 1.0 mL / min, column temperature of 30°C, and identification wavelength of : 0min~11min, 283nm; 11min~20min, 260nm; 20min~30min,...

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Abstract

The invention relates to a rhizoma drynariae crude product and a UPLC fingerprint spectrum construction and identification method thereof. The construction method comprises the following steps: respectively preparing a reference substance solution and a test substance solution, and detecting the reference substance solution and the test substance solution by adopting a high performance liquid chromatography, wherein the step of preparing the reference substance solution comprises the steps of taking reference substances, adding a solvent and dissolving the reference substances, and the reference substances comprise 5-hydroxymethylfurfural, protocatechuic acid, neoeriocitrin and naringin; and the step of preparing the test solution comprises the following steps: taking a rhizoma drynariae processed product, adding a solvent, extracting, filtering and collecting filtrate, wherein the rhizoma drynariae processed product is a rhizoma drynariae microwave processed product. According to the present invention, the obtained fingerprint can simultaneously contain the characteristic peaks of the rhizoma drynariae crude product and the different processed products thereof, and can completely and comprehensively reflect the chemical component information of the rhizoma drynariae crude product and the different processed products thereof, such that the fingerprint can be simultaneously used for identifying the rhizoma drynariae crude product and the various rhizoma drynariae processed products, and the application range is wide.

Description

technical field [0001] The invention relates to the field of detection and identification of processed traditional Chinese medicines, in particular to a method for constructing and identifying UPLC fingerprints of dried rhizomes and their processed products. Background technique [0002] Rhizoma Drynaria fortune (Kunze) J.Sm. is the dry rhizome of Drynaria fortune (Kunze) J.Sm., which has the effects of healing and relieving pain, tonifying the kidney and strengthening the bone, and for external use to eliminate wind and freckles. Modern pharmacological studies have shown that drynaria has significant curative effects on osteoporosis, bone injury repair, tooth protection, kidney protection, anti-inflammatory and blood fat reduction, cartilage protection, anti-myocardial ischemia and anti-virus. [0003] The traditional drynaria processing method is mainly sand scalding. In recent years, with the development of traditional Chinese medicine processing technology, microwave pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/32G01N30/54G01N30/74
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/32G01N30/54G01N30/74G01N2030/324
Inventor 黄瑶张正崔婷程钰洁甘力帆罗文汇刘涛乾钟霞孙冬梅魏梅程学仁
Owner GUANGDONG YIFANG PHARMA
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