Method and device for collecting and extracting skin tissues and preparing cell suspension

A cell suspension and skin technology, applied in the field of cell suspension preparation, can solve problems such as slow growth of autologous grafts, and achieve the effects of improving scar quality, increasing expansion rate, and improving cure rate

Inactive Publication Date: 2021-06-15
王忠坤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these autografts grow slowly and take time to develop the graft

Method used

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  • Method and device for collecting and extracting skin tissues and preparing cell suspension
  • Method and device for collecting and extracting skin tissues and preparing cell suspension
  • Method and device for collecting and extracting skin tissues and preparing cell suspension

Examples

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Embodiment 1

[0062] Example One: The first aspect of the present invention provides a method of preparing a cell suspension suitable for repair and regeneration of damaged tissue, according to which the tissue (preferably tissue of autologous origin). Preferably, this is achieved by performing a tissue biopsy. As biopsies are harvested, the depth and surface area of ​​the biopsy must be considered. The depth and size of the biopsy affect how easily surgery can be performed and how quickly the patient recovers from surgery. In a highly preferred form of the invention, the donor site should be selected to suitably match the recipient site, e.g. for head and neck post training, thigh for lower extremity, inner upper arm for upper extremity, or palm only or vice versa. Once the biopsy is taken from the patient, the tissue sample is subjected to physical and / or chemical dissociation means capable of dissociating the layers of cells in the tissue sample. Methods for dissociating cell layers ...

Embodiment 2

[0063] Embodiment 2: According to the second aspect of the present invention, an aqueous cell suspension prepared by the method described in the first aspect of the present invention is provided. The cell suspension provided by this method is well suited for tissue regeneration and transplantation techniques. An important advantage of using this suspension in transplantation techniques is that it can be used to greatly expand the size or volume of wounds that can be rapidly healed by in situ proliferation of limited numbers of cells. By varying the concentration of cells in the suspension, or by varying the amount of suspension distributed over a given area or volume of the transplant site, the number and concentration of cells seeded at the transplant site can be varied. By suspending the cells in at least (i) a nutrient solution free of gene X serum, (ii) able to maintain the viability of the cells until application to the patient, and (iii) suitable for direct application t...

Embodiment 3

[0064] Embodiment 3: According to the third aspect of the present invention, a method for treating a patient in need of tissue transplantation is provided. By this method, a cell suspension prepared according to the first aspect of the invention is applied to the site of transplantation. Liquid suspensions containing cells can be manually dispensed onto the graft site by any of a variety of techniques, including spraying, spreading, pipetting, and painting. In a highly preferred form of the invention, the suspension is sprayed onto the graft site. Suspensions can be sprayed through any type of nozzle that transforms the liquid into small airborne droplets. This embodiment suffers from two constraints. First, it cannot subject cells in solution to shear or stress that would damage or kill large numbers of cells. Second, there is no need to mix the cell suspension with a propellant fluid that is toxic or harmful to the cells or the wound bed. The variety of commonly available ...

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Abstract

The invention discloses a method and device for collecting and extracting skin tissues and preparing cell suspension. The method comprises the following steps: S1, subjecting a donor skin tissue sample comprising a corium layer and an epidermal layer to a dissociation device comprising an enzyme for dissociating the corium layer and the epidermal layer at a corium-epidermal junction in the skin tissue sample, wherein the donor skin tissue sample provides an expansion rate in therapy of 1:10 to 1:80; and S2, taking the donor skin tissue sample out of the dissociation device used in the step S1 and immersing the donor skin tissue sample in a nutrient solution storage device, wherein nutrient solution does not contain serum heterogeneity to the patient, can maintain cell viability before being applied to the patient and is suitable for being directly applied to the acceptor area of the patient. The problem of graft preparation time is solved; the expansion rate of the cell coverage rate is increased; the cure rate of small burns is improved; the method is useful for small-area skin reconstruction (such as scars); and the scar quality is improved.

Description

technical field [0001] The invention relates to a method, in particular to a method and device for collecting and extracting skin tissue and preparing a cell suspension, and belongs to the technical field of cell suspension preparation. Background technique [0002] Many methods of treating wounds are known to those skilled in the art. For example, there are skin grafting techniques that aim to reconstruct the skin covering areas of the body that have been damaged or deficient in the skin. Typically, these types of grafts are classified according to their host-donor relationship and their thickness. The most clinically used graft is an autograft, in which tissue is taken from one area of ​​the body and applied to another. The transplanted tissue then forms a new blood supply and attaches to the underlying tissue. Several types of skin grafts are currently used, including split-thickness, full-thickness grafts, and micrografts. Each of these graft types must be prepared u...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0625C12N5/0629C12N5/0626C12N5/0656C12N2509/00
Inventor王忠坤
Owner王忠坤