Process for fixing macromolecular substance on chip

A technology of polymer substances and chips, which is applied in the direction of analyzing materials, analyzing materials through electromagnetic means, measuring devices, etc., to achieve the effect of stable quality

Active Publication Date: 2021-06-18
FOSHAN MICROWONDERS BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention intends to provide a process for immobilizing polymer substances on a chip to solve the problem of how to fix biomolecules on electrode sheets

Method used

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Embodiment 1

[0027] A process for immobilizing polymer substances on a chip, including a chip activation step, a chip film forming step, a chip crosslinking step, and a chip coating step. The chip includes electrode sheets. The chip in this embodiment is selected from Chinese patent CN104965081B, patent name It is the reaction unit in the "antibody antigen detection method based on mobile equipment". The reaction unit includes a reaction chamber with an open top. The end passes through and is fixed on the reaction chamber and the box body. In this embodiment, the structure of the detection plate laid with electrode sheets can be referred to the inventor's previous paper (Development of an AC electronics-based immunoassay system for on-site serodiagnosis of infectious diseases, Xiaozhu Liu, Sensors and Actuators A, 171 (2011 ) 406–413, Fig. 3. (b)). Usually, the reaction chamber and the detection board are made of silicon (si), and the electrodes are made of metal (aluminum, gold or copper...

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Abstract

The invention relates to the field of biochips, and discloses a process for fixing a macromolecular substance on a chip, the process comprises a chip activation step, a chip film forming step and a chip crosslinking step, and the chip comprises an electrode plate; in the chip activation step, air is used as a medium to carry out plasma cleaning on a chip, and an activated chip is obtained; and in the chip film forming step, APTES is covered on the electrode plate, and the chip after film forming is obtained. According to the invention, the biomolecules can be well fixed on the chip, especially well fixed on the electrode plate of the chip, so that the chip can be used in a biochip detection method based on electrical detection, and the development of the biochip detection method based on electrical detection is promoted. In addition, the finished chips produced in the same batch are stable in quality and good in repeatability.

Description

technical field [0001] The invention relates to the field of biochips, in particular to a process for immobilizing polymer substances on a chip. Background technique [0002] Biochip is based on the principle of specific interaction between biomolecules, and integrates the biochemical analysis process on the surface of the chip, so as to realize high-throughput and rapid detection of DNA, peptides, proteins and other biological components. The narrow concept of biochip refers to the immobilization of biomolecules (oligonucleotides, cDNA, gDNA, polypeptides, antibodies, antigens, etc.) The biomolecular lattice formed on it. In the traditional biochip detection method, the specific binding between the target biomolecules and the corresponding biomolecules coated on the biochip simply relies on diffusion motion and random Brownian motion, so the detection time is relatively long. In order to shorten the time of biochip detection and improve the efficiency of biochip detection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/00G01N27/02
CPCG01N27/00G01N27/02
Inventor 徐海刘晓竹李俊
Owner FOSHAN MICROWONDERS BIOTECHNOLOGY CO LTD
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