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Buffer solution and application thereof

A technology of buffer solution and solution, which is applied in the field of buffer solution and can solve problems such as electrode sheet corrosion

Active Publication Date: 2021-06-18
FOSHAN MICROWONDERS BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention intends to provide a buffer solution to solve the technical problem that the electrode sheet of the chip is prone to corrosion due to the processing method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Chip processing

[0018] This embodiment is a finished chip that forms an antibody of the antibody on the basis of unmisting a chip of a molecule (antibody or antigen or other affinity molecule).

[0019] The chip used in this example see the inventors in the prior patent CN104965081B (based on the antigenic antigen detection method of the mobile device), in this patent, there is (and refer to Fig. 2 of this patent): an antibody antigen detection test system, including At least one reaction unit, the reaction unit includes a reactive chamber of a top opening, a detecting plate in the bottom of the reaction chamber, and at least one pair of electrode sheets are laid on the detecting plate, and the terminal of the electrode sheet passes through and fixed in the reaction chamber. On; the corresponding antigen or antibody of the target antibody or antigen is also fixed on the detection plate. In the present embodiment, the chip specifically refers to the reaction uni...

Embodiment 2

[0035] Example 2: Chip processing

[0036] The present embodiment is improved on the basis of Example 1, and the quality control process of impedance scanning is added during the "5. Chip Bag" process to ensure the good product of the chip. details as follows:

[0037] Chipbag

[0038] 10 ul 10 μg / ml commercialized Brusique OMP16 antigen (a specific package is used for dispersing and dissolving the molecular solution, and the solvent for dispersing and dissolving the molecule is 100 mm BBs, according to actual needs The case of molecules can be selected from 5 min (optional range 2 to 10 min).

[0039] The impedance frequency scan measurement and analysis is then used to connect to the terminal of the electrode sheet using the impedance. The scan frequency range is 1MHz to 100 Hz (sinusoidal AC), the excitation voltage is 5mV (optional range 1mv ~ 100mv), the sample point number is 201 point, the measurement time is 3s, and the chip impedance frequency scan data (called packet p...

experiment example 1

[0043] Experimental Example 1: Study on corrosion corrosion effect of finished chip

[0044] In this first experiment, the buffer in Example 1 was studied in the buffer in Example 1, and the specific manner is to use a test buffer instead of the boric acid buffer of the example, and passed the implementation The method of the example obtains the finished chip. The finished product chip that is just prepared is placed in a dry sealing bag (the number 1-9 test uses 10 finished chips, all of which are packaged in the dry sealing bag), daily use of the gold phase microscope to observe the chip under 10 times the eyepiece The surface (mainly electrode sheet), determines whether or not the electrode sheet has corrosion or corrosion, if there is a date, the date of this phenomenon occurs, and the loss of rust-rust, and the experimental results are shown in Table 1.

[0045] Table 1: Test results of the resistance of rust (Mean ± SD, N = 10)

[0046] Numbering Test buffer type ...

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PUM

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Abstract

The invention relates to the technical field of molecule rapid detection element processing, in particular to a buffer solution and application thereof. The buffer solution comprises the following raw materials: a boric acid solution with the concentration of 0.05 to 0.2 M and a sodium tetraborate solution with the concentration of 0.0125 to 0.05 M, and the pH value of the buffer solution is 5 to 8. The buffer solution is applied to practical operation of processing and production of a molecular detection chip, and can solve the technical problem that a chip electrode plate is easy to corrode due to a processing method, so that the validity period of the chip is prolonged, the quality and yield of the chip are improved, and the accuracy and stability of related detection are improved.

Description

Technical field [0001] The present invention relates to the field of molecular rapid detecting element processing techniques, and more particularly to a buffer and its application thereof. Background technique [0002] The principle of immune response and affinity response is now widely used in the practice of molecular detection or diagnosis, but during the detection process, the binding of the molecules is carried out in a passive manner such as thermal diffusion, resulting in slow reaction speed and detection effect. difference. The inventors of the present scheme proposes a device and method that actively controls the binding reaction between the above molecules, overcomes the defects in the prior art, detailed in China Patent CN104965081B (based on mobile device antigen antigen detection method) Content. A particularly paving chip is used in this scheme to realize the control of molecular motion by applying a certain voltage and an alternating current at the electrode sheet....

Claims

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Application Information

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IPC IPC(8): G01N27/00G01N27/02G01N33/543G01N33/531
CPCG01N27/00G01N27/02G01N33/543G01N33/531
Inventor 徐海刘晓竹李俊
Owner FOSHAN MICROWONDERS BIOTECHNOLOGY CO LTD
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