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A kind of buffer solution and its application

A technology of buffer solution and solution, which is applied in the field of buffer solution and can solve problems such as electrode sheet corrosion

Active Publication Date: 2022-01-04
FOSHAN MICROWONDERS BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention intends to provide a buffer solution to solve the technical problem that the electrode sheet of the chip is prone to corrosion due to the processing method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: chip processing

[0018] In this embodiment, a finished chip coated with antibodies is processed on the basis of unfixed coated molecules (antibodies or antigens or other affinity molecules).

[0019] For the chip used in this example, refer to the inventor’s previous patent CN104965081B (antibody antigen detection method based on mobile equipment), which records (and refer to the accompanying drawing 2 of the patent): an antibody antigen detection test system, including At least one reaction unit, the reaction unit includes a reaction chamber with an open top, a detection board is arranged at the bottom of the reaction chamber, at least a pair of electrode sheets are laid on the detection board, and the terminals of the electrode sheets pass through and are fixed in the box of the reaction chamber Above; the corresponding antigen or antibody of the target antibody or antigen is immobilized on the detection plate. In this embodiment, the chip specifically...

Embodiment 2

[0035] Embodiment 2: chip processing

[0036] This embodiment is improved on the basis of Embodiment 1, and the quality control process of impedance scanning is added in the process of "5. Chip coating" to ensure the yield rate of the chip. details as follows:

[0037] chip coating

[0038] Add 10ul 10μg / ml commercialized Brucella omp16 antigen (a specific coating molecule solution, the solvent used to disperse and dissolve the coating molecule is 100mM BBS) on the chip after cross-linking, and detect according to actual needs Depending on the molecular situation, different antigens can be used), and incubated for 5 minutes (the range of choice is 2 to 10 minutes).

[0039] Then use an impedance meter to connect to the terminal of the electrode sheet for impedance frequency scanning measurement and analysis. The scanning frequency range is 1MHz to 100Hz (sinusoidal alternating current), the excitation voltage is 5mV (the range of 1mV to 100mV can be selected), the number of...

experiment example 1

[0043] Experimental example 1: Research on the anti-corrosion effect of finished chips

[0044] This experimental example studies the buffer solution in "5. Chip coating" and "6. Blocking and drying" of Example 1. The specific method is to use the test buffer instead of the boric acid buffer in the example, and by implementing The method of the example is used to prepare the finished chip. Put the newly prepared finished chip in a dry sealed bag (tests No. 1-9 used 10 finished chips, all packaged in a dry sealed bag), and use a metallographic microscope to observe the chip under a 10x eyepiece every day Surface (mainly the electrode sheet), judge whether the electrode sheet is corroded or rusted, if it occurs, record the date of the phenomenon, and count the anti-corrosion time. The experimental results are shown in Table 1.

[0045] Table 1: Anti-corrosion duration test results (mean±SD, N=10)

[0046] Numbering Test Buffer Type Test buffer preparation method ...

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Abstract

The invention relates to the technical field of molecular rapid detection element processing, in particular to a buffer solution and an application thereof. A buffer solution, the raw materials of which include 0.05-0.2M boric acid solution and 0.0125-0.05M sodium tetraborate solution, and the pH value is 5-8. The buffer solution is applied in the practical operation of the processing and production of molecular detection chips, which can solve the technical problem that the electrode pads of the chip are easily corroded due to the processing method, thereby prolonging the validity period of the chip, improving the quality of the chip, the yield rate and improving the quality of the chip. The accuracy and stability of the relevant detection.

Description

technical field [0001] The invention relates to the technical field of molecular rapid detection element processing, in particular to a buffer solution and an application thereof. Background technique [0002] The principles of immune reaction and affinity reaction have been widely used in the practice of molecular detection or diagnosis, but in the detection process, the combination of molecules is carried out by passive methods such as thermal diffusion, resulting in slow reaction speed and poor detection effect. Difference. The inventor of this solution proposed a device and method that can actively control the binding reaction between the above molecules, which overcomes the defects in the prior art. For details, see Chinese patent CN104965081B (antibody antigen detection method based on mobile equipment) Content. In this scheme, a special chip with electrode sheets is used, and the control of molecular motion is realized by applying a certain voltage and frequency of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/00G01N27/02G01N33/543G01N33/531
CPCG01N27/00G01N27/02G01N33/543G01N33/531
Inventor 徐海刘晓竹李俊
Owner FOSHAN MICROWONDERS BIOTECHNOLOGY CO LTD
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