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Rapid detection method for balance stability of folic acid content in infant complementary food nutrition bag

A detection method and technology of nutritional package, which are applied in measurement devices, instruments, fluorescence/phosphorescence, etc., can solve problems such as method research on balanced stability of folic acid-rich products that have not been seen

Active Publication Date: 2021-06-18
GANZHOU QUANBIAO BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Throughout the research literature on the method of folic acid detection, there is no research on the method of equilibrium stability in folic acid-rich products, and there is no method for quickly detecting and judging the equilibrium stability of folic acid in the production product between the national standard microbial method and the HPLC method. Related reports

Method used

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  • Rapid detection method for balance stability of folic acid content in infant complementary food nutrition bag
  • Rapid detection method for balance stability of folic acid content in infant complementary food nutrition bag
  • Rapid detection method for balance stability of folic acid content in infant complementary food nutrition bag

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Precisely weigh 0.0117g folic acid standard (C 19 H 19 N 7 O 6 Purity is 97%) in a 50mL volumetric flask, add an appropriate amount of 0.5% ammonia water, use ultrasonic frequency 40kHz to perform ultrasonic treatment for 10min to completely dissolve folic acid, and then use 0.5% ammonia water to make up to the mark to obtain a concentration of 213.74μg / mL of folic acid standard product stock solution (mother liquor); then dilute the folic acid standard stock solution (mother liquor) with water to dilute 4000 times, 2000 times, 400 times, 200 times, and 100 times of the folic acid standard solution with concentration gradients, respectively, carry out liquid chromatography HPLC detection, The test conditions are to select 250mm×4.6mm, 5μm C 18 Chromatographic column and fluorescence detector, detection wavelength is λ ex =344nm and λ em =428nm, mobile phase A is acetonitrile, mobile phase B is 0.05mol / L potassium dihydrogen phosphate aqueous solution, flow rate is ...

Embodiment 2

[0041] Embodiment 2: Accurately weigh 0.0114g folic acid standard (C 19 H 19 N 7 O 6 Purity 97%) in a 50mL volumetric flask, add an appropriate amount of 0.5% ammonia water, use ultrasonic frequency 20kHz to perform ultrasonic treatment for 30min to completely dissolve the folic acid, and then use 0.5% ammonia water to make up to the mark to obtain a folic acid standard with a concentration of 228.33μg / mL Stock solution (mother liquor); then the folic acid standard stock solution (mother liquor) is diluted with water respectively 4000 times, 2000 times, 400 times, 200 times, 100 times of the folic acid standard solution of the concentration gradient for subsequent use, respectively carries out liquid chromatography HPLC detection, The test conditions are to select 250mm×4.6mm, 5μm C 18 Chromatographic column and fluorescence detector, detection wavelength is λ ex =344nm and λ em =428nm, mobile phase A is acetonitrile, mobile phase B is 0.05mol / L potassium dihydrogen phosp...

Embodiment 3

[0043] Precisely weigh 0.0101g folic acid standard (C 19 H 19 N 7 O 6 Purity 97%) in a 50mL volumetric flask, add an appropriate amount of 0.5% ammonia water, use ultrasonic frequency 30kHz for ultrasonic treatment for 20min to completely dissolve folic acid, and then use 0.5% ammonia water to make up to the mark to obtain a concentration of 202.3μg / mL of folic acid standard Stock solution (mother liquor); then the folic acid standard stock solution (mother liquor) is diluted with water respectively 4000 times, 2000 times, 400 times, 200 times, 100 times of the folic acid standard solution of the concentration gradient for subsequent use, respectively carries out liquid chromatography HPLC detection, The test conditions are to select 250mm×4.6mm, 5μm C 18 Chromatographic column and fluorescence detector, detection wavelength is λ ex =344nm and λ em =428nm, mobile phase A is acetonitrile, mobile phase B is 0.05mol / L potassium dihydrogen phosphate aqueous solution, flow rat...

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Abstract

The invention relates to a rapid detection method for the balance stability of folic acid content in an infant complementary food nutrition bag, which belongs to the technical field of infant complementary food nutrition bag product creation. The invention discloses a rapid detection method for detecting the folic acid content of an infant complementary food nutrition bag based on a liquid chromatography HPLC method and a folic acid content equilibrium stability judging model Y=0.876X+11.131, a model R<2>=0.944 and a P value=0.000<0.05. The model is remarkable at the verification level of 0.05, the root mean square error RMSE of the model is equal to 2.245843, and the standard deviation between a measured value and a predicted value is smaller than or equal to 0.768024. The method not only can be widely applied to the field of rapid detection of the folic acid content in folic acid-rich fortified food, but also can be applied to detection of the folic acid fortified equilibrium stability state in different batches of infant complementary food nutrition bags, and can rapidly guide production to improve the superior product rate of the infant complementary food nutrition bags.

Description

technical field [0001] The invention belongs to the technical field of the creation of nutritional supplements for infants and young children, and relates to a rapid detection method for the balance and stability of folic acid content in nutritional supplements for infants and young children, and a method for judging whether the nutritional packages for infants and young children rich in folic acid are in products under production or storage environments. A rapid test method for the equilibrium stability of folic acid content. Background technique [0002] Folic acid, also known as pteroylglutamic acid, is composed of three parts: 2-amino-4-hydroxy-6-methyl-pterinidine, glutamic acid and p-aminobenzoic acid. As a methyl donor, it is involved in many important The biochemical reaction process, especially in the synthesis and repair of vitamin B12 and vitamin B6, as well as the synthesis of purine pyrimidine and methylated DNA, plays an important role. Folic acid cannot be sy...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86G01N21/64
CPCG01N30/02G01N30/06G01N30/34G01N30/8675G01N21/64
Inventor 钟利敏林松毅吴超祁立波谢良星朱春燕尚珊姜鹏飞傅宝尚袁鹏
Owner GANZHOU QUANBIAO BIOTECH
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