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Compound microbial agent for reducing aspergillus flavus infection and pollution of grain and oil crops as well as preparation method and application of compound microbial agent

A technology of compound microbial inoculum and crops, which is applied in the field of compound microbial inoculum and its preparation, and achieves the effects of small bulk density, increased utilization rate, and improved quality.

Inactive Publication Date: 2021-06-22
AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many microbial agent products on the market, but there are very few compound microbial agent products specifically for the control of Aspergillus flavus infection and toxin pollution on grain and oil crops

Method used

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  • Compound microbial agent for reducing aspergillus flavus infection and pollution of grain and oil crops as well as preparation method and application of compound microbial agent

Examples

Experimental program
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Effect test

Embodiment 1

[0083] Example 1. Composite microbial agent for reducing the infection of grain and oil crops by Aspergillus flavus and its toxin pollution and its preparation The composite microbial agent of this embodiment is composed of the following raw materials in mass percentage:

[0084] Bacillus amyloliquefaciens: 1.5%, Enterobacter ludwigii: 2%, Trichoderma konii 1: 1%, Trichoderma konii 2: 1%, Potassium dihydrogen phosphate 1%, Potassium nitrate 2.5%, Magnesium sulfate 1%, wheat bran 50%, corn flour 25%, meat and bone meal 15%. Among them, the obtained composite microbial agent has a viable count of 500 million / g and consists of 4 functional microorganisms: Bacillus amyloliquefaciens, Enterobacter ludwig, Trichoderma konshii 1 and Trichoderma konshii 2,4 The ratio of the number of live bacteria of each type of microorganism is 1.5:2.0:1.0:1.0.

[0085] The specific preparation method is:

[0086] (1) According to the number of live bacteria of the 4 kinds of functional microbial ...

Embodiment 2

[0094] Example 2, Composite microbial agent for reducing the infection of grain and oil crops by Aspergillus flavus and its toxin pollution and its preparation The composite microbial agent of this embodiment is composed of the following raw materials in mass percentage:

[0095] Bacillus amyloliquefaciens: 1.5%, Enterobacter ludwigii: 2%, Trichoderma konii 1: 1%, Trichoderma konii 2: 1%, Potassium dihydrogen phosphate 1%, Potassium nitrate 2.5%, Magnesium sulfate 1%, wheat bran 50%, corn flour 25%, meat and bone meal 15%. Among them, the obtained composite microbial agent has a viable count of 800 million / g and consists of 4 functional microorganisms: Bacillus amyloliquefaciens, Enterobacter ludwig, Trichoderma konii 1 and Trichoderma konshii 2,4 The ratio of the number of live bacteria of each type of microorganism is 1.5:2.0:1.0:1.0.

[0096] The specific preparation method is:

[0097] (1) According to the number of live bacteria of the 4 kinds of functional microbial po...

Embodiment 3

[0104]Example 3, Composite microbial agent for reducing the infection of grain and oil crops by Aspergillus flavus and its toxin pollution and its preparation The composite microbial agent of this embodiment is composed of the following raw materials in mass percentage:

[0105] Bacillus amyloliquefaciens: 1.5%, Enterobacter ludwigii: 2%, Trichoderma konii 1: 1%, Trichoderma konii 2: 1%, Potassium dihydrogen phosphate 1%, Potassium nitrate 2.5%, Magnesium sulfate 1%, wheat bran 50%, corn flour 25%, meat and bone meal 15%. Among them, the obtained composite microbial agent has a viable count of 800 million / g and consists of 4 functional microorganisms: Bacillus amyloliquefaciens, Enterobacter ludwig, Trichoderma konii 1 and Trichoderma konshii 2,4 The ratio of the number of live bacteria of each type of microorganism is 1.5:2.0:1.0:1.0.

[0106] The specific preparation method is:

[0107] (1) According to the number of live bacteria of the 4 kinds of functional microbial pow...

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Abstract

The invention discloses a compound microbial agent for reducing aspergillus flavus infection and toxin pollution of grain and oil crops. The microbial agent is prepared from the following raw materials in percentage by mass: 1 to 1.5 percent of bacillus amyloliquefaciens, 2 to 2.5 percent of enterobacter ludwigii, 0.5 to 1 percent of trichoderma koningii 1 and 0.5 to 1.5 percent of trichoderma koningii 2, 0.5 to 1.5 percent of monopotassium phosphate, 1.5 to 2.5 percent of potassium nitrate, 0.5 to 1.5 percent of magnesium sulfate, 45 to 55 percent of wheat bran, 25 to 35 percent of corn flour and 13.5 to 20 percent of meat bone powder. The compound microbial agent can inhibit fungal diseases such as toxin-producing aspergillus flavus in soil, reduce aflatoxin pollution in agricultural products in the middle and later periods and improve the quality and quality safety of the agricultural products, and meanwhile, a plurality of trace elements and microorganisms are added, so that the soil fertility is improved, the degradation of harmful residues in soil is accelerated, the continuous cropping obstacle of crops is overcome, and the like.

Description

technical field [0001] The invention belongs to the field of functional microorganism application, and in particular relates to a compound microbial bacterial agent for reducing the infection of aspergillus flavus and toxin pollution of grain and oil crops, its preparation method and application. Background technique [0002] Grain and oil crops and products are susceptible to mycotoxin contamination during growth, storage and transportation, especially aflatoxin, which will seriously affect the quality and safety of agricultural products and food safety. How to solve the problem of aflatoxin pollution in agricultural products has become a worldwide problem to be solved urgently. Aflatoxins are a type of strong carcinogenic and highly toxic mycotoxins produced by fungi such as Aspergillus flavus or Aspergillus parasiticus. Human or animal consumption of toxin-contaminated food or feed will cause body disease or even death, which seriously threatens the health and safety of c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/20A01N63/38A01P3/00A01C1/06A01C1/08A01C13/00A01C21/00A01G22/15A01G22/20A01G22/22A01G22/40C12R1/07C12R1/885
CPCC12N1/20C12N1/14C05F11/08C05G3/00C05G3/60A01N63/22A01N63/20A01N63/38A01C21/005A01C1/06A01C1/08A01C13/00A01G22/40A01G22/20A01G22/22A01G22/15
Inventor 姚彦坡黄艳红韩鹏裴广芬杜兆林高东霞强沥文陈益胜林大松秦丽霍莉莉王伟安毅
Owner AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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