Preparation method of personalized active compound microbial agent for purifying sewage and water body
A technology of composite microbial agent and microbial flora, applied in the field of natural microbiology, can solve problems such as deterioration of water environment and difficult treatment, and achieve the effects of promoting proliferation, promoting metabolic reactions, and improving stability
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[0034] The invention designs the preparation method of the personalized active composite microbial agent for purifying sewage water. In order to customize the bacterial agents that meet their individual needs, firstly, a normal sewage water microbial database was constructed, and the distribution ratio information of the microbial composition in the 30 sewage water samples was obtained through high-throughput 16SrRNA gene sequencing (the process is as follows: figure 1 with 2 shown), then determine the dynamic bacterial formulation according to the following method (such as image 3 shown). The test is as follows:
[0035] (1) Water sample treatment: extract and purify the microbial DNA in the sample by bead breaking method, and use 515F / 806R PCR primers to amplify the V4 hypervariable region of the 16S rRNA gene of microbial DNA; 515F / 806R PCR primers For 515F: GTGCCAGCMGCCGCGGTAA, 806R: GGACTACHVGGGTWTCTAAT.
[0036] (2) Determination of the total amount of microorganism...
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