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Preparation method of personalized active compound microbial agent for purifying sewage and water body

A technology of composite microbial agent and microbial flora, applied in the field of natural microbiology, can solve problems such as deterioration of water environment and difficult treatment, and achieve the effects of promoting proliferation, promoting metabolic reactions, and improving stability

Pending Publication Date: 2021-06-22
南京尧玥生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the sewage in the middle of each year will produce a large amount of pollutants, leading to the deterioration of the water environment
Due to the huge volume of sewage water, especially the point source and high concentration characteristics of industrial sewage, it is very difficult to treat

Method used

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  • Preparation method of personalized active compound microbial agent for purifying sewage and water body
  • Preparation method of personalized active compound microbial agent for purifying sewage and water body
  • Preparation method of personalized active compound microbial agent for purifying sewage and water body

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Embodiment example

[0034] The invention designs the preparation method of the personalized active composite microbial agent for purifying sewage water. In order to customize the bacterial agents that meet their individual needs, firstly, a normal sewage water microbial database was constructed, and the distribution ratio information of the microbial composition in the 30 sewage water samples was obtained through high-throughput 16SrRNA gene sequencing (the process is as follows: figure 1 with 2 shown), then determine the dynamic bacterial formulation according to the following method (such as image 3 shown). The test is as follows:

[0035] (1) Water sample treatment: extract and purify the microbial DNA in the sample by bead breaking method, and use 515F / 806R PCR primers to amplify the V4 hypervariable region of the 16S rRNA gene of microbial DNA; 515F / 806R PCR primers For 515F: GTGCCAGCMGCCGCGGTAA, 806R: GGACTACHVGGGTWTCTAAT.

[0036] (2) Determination of the total amount of microorganism...

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Abstract

The invention discloses a preparation method of a personalized active microbial agent and a derivative thereof for purifying a sewage water body and an application thereof, and a large amount of pollutants such as impurities, waste residues, free nitrogen ions and the like in the sewage water body are immobilized and concentrated through extracellular polypeptides and microbial thalli of microorganisms with an adhesion function; the formed complex of bacteria, derivatives and pollutants is effectively intercepted and applied to purification of sewage water body. The active compound microbial strains are all from various natural strains in the natural environment. According to a biological information analysis and management platform, an active compound microorganism strain formula is optimized and perfected, active beneficial symbiotic microorganism strains are inoculated, fermentation is performed, and harmless treatment is performed. Under the action of optimizing and personally forming the active microbial strain, the high-quality active microbial agent and the derivative thereof are obtained, and the harm of adding any chemical reagent and the like is avoided. Due to some organic substances and toxic substances which are difficult to degrade, the organic substances and the toxic substances need to be treated by a microbial method. Microorganisms can obtain nutrients, and meanwhile, harmful substances are degraded and utilized, so that the harmful substances are purified. The microbiological treatment technology has the remarkable advantages of low consumption, high efficiency, low cost, convenience and reliability in process operation management, no secondary pollution and the like, and has a good application prospect.

Description

technical field [0001] The microorganisms used in the present invention are natural microorganisms. Microorganisms reproduce in large numbers by consuming pollutants in sewage, so as to have a better purification effect on sewage, and also reduce the damage of pollutants to the natural water environment, which can prevent the water environment from being polluted. The microorganisms used in the invention can effectively eliminate the trouble of stench, prevent the breeding of pathogenic bacteria, mosquitoes and flies, and solve the problems of water pollution and the like. [0002] More specifically, the present invention relates to the formulation method and precise application of the micro-ecological personalized formula for purifying sewage water body. Background technique [0003] The quality of life in modern society is constantly improving, and people's demand for water quality in basic necessities of life is increasing. Therefore, the sewage in the middle of each ye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/04C12N1/20C12N1/16
CPCC12Q1/689C12N1/20C12N1/16
Inventor 尹星任淑媛吴欣圆徐新燕孙子茜
Owner 南京尧玥生物科技有限公司
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