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A method for rapid propagation of high-quality seedlings of Safflower tissue culture

A tissue culture, safflower banana technology, applied in the field of plant tissue culture, can solve the problems of no safflower banana, high pollution rate, slow reproduction speed and the like, and achieves the effects of simple and affordable technology and high application value

Active Publication Date: 2022-03-04
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the natural state, Acacia safflower is mainly propagated by ramets, the propagation speed is slow, and it is difficult to meet the needs of the market. The use of plant tissue culture technology can effectively solve this problem, but when the underground rhizomes of Acacia safflower are used as explants , high pollution rate
[0003] At present, there is no report on the propagation of safflower seedlings by tissue culture at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. The acquisition and disinfection method of explants: select the unopened young flower buds of the excellent line of Musa coccinea Andr. which grow vigorously and have no damage by diseases and insect pests in the growing season, and first soak them in 75% alcohol by volume fraction. Soak for 20 seconds, then disinfect with 0.1% mercury chloride solution for 8 minutes, rinse with sterile water for 4 times, then disinfect with 0.1% mercury chloride solution for 4 minutes, rinse with sterile water for 5 times, and insert the callus In the tissue induction medium, the culture temperature is 24° C., the light intensity is 1500 lx, and the light is 16 hours / day. After 40 days of culture, there are calluses and somatic embryos at the base of the explants, and the sterilization success rate is 85%. Each liter of callus induction medium contains: 6-benzylpurine (6-BA) 1.0 mg, thidiazuron (TDZ) 0.5 mg, kinetin (KT) 1.0 mg, coconut water 100 ml, sucrose 20 g, agar 6 g, the bala...

Embodiment 2

[0026] 1. The acquisition and disinfection method of explants: select the unopened young flower buds of the excellent line of Musa coccinea Andr. which grow vigorously and have no damage by diseases and insect pests in the growing season, and first soak them in 75% alcohol by volume fraction. Soak for 30 seconds, then disinfect with 0.1% mercury solution for 9 minutes, rinse with sterile water for 5 times, then disinfect with 0.1% mercury solution for 3 minutes, rinse with sterile water for 4 times, insert the callus In the tissue induction medium, the culture temperature is 28°C, the light intensity is 2000 lx, and the light is 14 hours / day. After 35 days of culture, there are calluses and somatic embryos at the base of the explants, and the sterilization success rate is 90%. Each liter of callus induction medium contains: 6-benzylpurine (6-BA) 1.5 mg, thidiazuron (TDZ) 0.3 mg, kinetin (KT) 1.5 mg, coconut water 75 ml, sucrose 25 g, agar 6.5 grams, the balance is MS medium, p...

Embodiment 3

[0032] 1. The acquisition and disinfection method of explants: select the unopened young flower buds of the excellent line of Musa coccinea Andr. which grow vigorously and have no damage by diseases and insect pests in the growing season, and first soak them in 75% alcohol by volume fraction. Soak for 40 seconds, then disinfect with 0.1% mercury liter solution for 10 minutes, rinse with sterile water for 5 times, then disinfect with 0.1% mercury solution for 2 minutes, rinse with sterile water for 5 times, insert the callus In the tissue induction medium, the culture temperature is 30°C, the illuminance is 2500 lx, and the light is 12 hours / day. After 30 days of culture, there are callus and somatic embryos at the base of the explants, and the sterilization success rate is 95%. Each liter of callus induction medium contains: 6-benzylpurine (6-BA) 2.0 mg, thidiazuron (TDZ) 0.2 mg, kinetin (KT) 2.0 mg, coconut water 50 ml, sucrose 30 g, agar 7 g, the balance being MS medium, pH ...

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Abstract

The invention discloses a method for rapid propagation of high-quality seedlings of safflower tissue culture. In the present invention, the unopened young flower buds of the excellent strain of Safflower banana are used as explants, and the explants are obtained and sterilized, callus proliferation and differentiation, somatic embryo differentiation to form plants, and adventitious buds to take root and cultivate strong seedlings. In the stages of transplanting and test-tube seedlings, etc., the medium formula and culture conditions suitable for each stage of cultivation were selected, and the large-scale propagation of safflower seedlings was successfully carried out, and high-quality safflower seedlings were bred to meet the needs of the market. The technology of the invention is simple and affordable, practical and has high application value. The implementation of the invention only needs simple plant tissue culture equipment to carry out.

Description

Technical field: [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rapid propagation of high-quality seedlings of safflower tissue culture. Background technique: [0002] Safflower banana (Musa coccinea Andr.), also known as red banana, zhitian banana, etc., is a perennial evergreen herbaceous plant in the Musa family, native to eastern Yunnan, Guangdong, Guangxi and other places in China. Banana safflower has a chic plant shape, large inflorescences, bright red bracts, and long-lasting flowering. It is an ornamental flower with high ornamental value. In warm regions such as South China, it can be planted in the corner of the courtyard, in front of the window, on a rockery, at the entrance of a pavilion or by a pool And other places, very full of southern characteristics, can also be potted for viewing; its bright and straight red erect inflorescences are also excellent flower arrangement materials, and can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾宋君李琳吴坤林房林
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI