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Marine anti-glioma natural active substance Streptomyces naphthyridine A as well as preparation and application thereof

The technology of a natural active substance, streptavidin, is applied in the field of medicine and can solve problems such as few researches

Active Publication Date: 2021-06-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many studies on microbial metabolites in the Mariana Trench, and there are only sporadic reports

Method used

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  • Marine anti-glioma natural active substance Streptomyces naphthyridine A as well as preparation and application thereof
  • Marine anti-glioma natural active substance Streptomyces naphthyridine A as well as preparation and application thereof
  • Marine anti-glioma natural active substance Streptomyces naphthyridine A as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Separation and culture of Streptomyces streptomyces Sp.SY2111

[0033] 7281 meters deep from the Mariana Huggou was dried at 28 ° C for 8 days. 1 gram of dried deposits were taken in 9 mL of sterile water, and the sterilized self-contained water was oscilted overnight to a concentration of 1 × 10 -3 G / ml sample solution. The 200 μl of sample fluid was taken to a petri dish containing a high agar solid medium, and after 7 days of culture at 28 ° C, different colonies were transferred to another petri dish containing a high agar solid medium. In the case of 28 ° C, it continued for 7 days. Finally, the growth of SY2111 single bachelor was inoculated into a high agar solid beveled medium, and the refrigerator was stored at a 4 ° C refrigerator.

Embodiment 2

[0034] Example 2 Streptomyces Streptomyces Sp.SY2111 of the strain identification

[0035] The species of the obtained strain SY2111 were identified using a 16S RDNA sequence analysis method.

[0036] 2.1 experimental reagents and instruments

[0037] PCR reagent: PrimeStar Max Dna Polymerase (Takara), primer (Invitrogen Synthesis), primer sequence is:

[0038]

[0039] Marker: DL5000

[0040] Experimental instruments: centrifuge, electrophoretic instrument, PCR, ABI 3730XL sequencer.

[0041] 2.2 Experimental steps

[0042] Bacterial genome DNA extraction

[0043] Electrophoretic test

[0044] PCR amplification

[0045] A.PCR reaction system

[0046]

[0047]

[0048] B.PCR reaction conditions

[0049]

[0050] C. Electrophoretic detection

[0051] d. Sequencing: Purification Sequencing

[0052] e. Analysis Result: Splicing sequence.

[0053] 2.3 Experimental results

[0054] The sequence after stitching is:

[0055] gtgttaccgactttcgtgacgtgacgagcggtgtgtacaaggcccgggaacgtattc...

Embodiment 3

[0057] Example 3 Preparation of Solid Fermentation Products Streptomystomyces Sp.SY2111 Solid Fermentation Product

[0058] The bacterium of Streptomymidus STREPTOMYCES SP.SY2111 is inoculated with a Gaoli agar solid beveled medium to contain 250 ml of liquid high medium (20 grams of soluble starch, 1 gram of nitrate, 0.5 grams of magnesium sulfate, 0.5 grams of hydrogen phosphate) In the 500 ml triangular flask containing 0.5 g of sodium chloride, iron sulfate 0.01 grams of sulfate, 1 liter of natural seawater), a culture solution containing SY2111 strains was rotated (180 rpm) to vibrate 4 days after 4 days of 4 days. . 5 ml of strain was transferred to a 500 ml triangular flask containing rice solid medium (40 grams of rice and natural seawater), allowed to culture for 60 days at 28 ° C, resulting in streptavuria containing anti-gluly prototype Nellin A culture. The total rice culture of 200 bottles of strain SY2111 was prepared in this experiment.

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Abstract

The invention provides a marine anti-glioma natural active substance Streptomyces naphthyridine A as well as preparation and application thereof, the marine anti-glioma natural active substance Streptomyces naphthyridine A is separated from a metabolite of streptomycete in marine sediments, the classification name of the streptomycete is Streptomyces sp.SY2111, and the preservation number is CCTCC M 2021154. The Streptomyces naphthyridine A can significantly inhibit proliferation of human glioma U87MG and U251 cells, can be used for preparing anti-glioma drugs, and provides a new drug molecule for treatment of glioma. The chemical structural formula of the Streptomyces naphthyridine A is shown in the specification.

Description

Technical field [0001] The present invention is a pharmaceutical field, and a new compound of new compound having anti-hydrauloma activity is obtained from the metabolites of the NOO EOG STREPTOMYCES SP.SY2111 and its preparation method and its preparation method and preparation anti-colloidal Application in tumor drugs. Background technique [0002] Grid is a common cerebral tumor with high mortality. Currently, the treatment of glioma mainly includes surgery resection, radiotherapy, and drug treatment based on monoxazole. However, since the glioma is invasive growth, there is no significant boundaries of tumor tissue and normal tissue, and the glioma is more important in the brain, so the surgery is unable to completely remove the lesion tissue. And radiotherapy is large. Therefore, the drug is more important for the treatment of gliomas. However, the drug currently used in glioma therapy is extremely limited, and the telromamide is the only drug that can be used alone in gliom...

Claims

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Application Information

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IPC IPC(8): C07D471/04A61P35/00C12N1/20C12P17/18C12R1/465
CPCC07D471/04A61P35/00C12N1/20C12P17/182
Inventor 张治针秦乐勇括连晓媛
Owner ZHEJIANG UNIV
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