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miRNA biomarkers and detection kits for the diagnosis of cervical lymph node metastasis in papillary thyroid carcinoma

A technology for lymph node metastasis and biomarkers, applied in the field of genetic engineering, to achieve the effects of quantitative accuracy, less trauma, and convenient detection

Active Publication Date: 2022-05-24
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no specific microRNA biomarker combination and detection kit for judging the metastasis of papillary thyroid carcinoma in lateral cervical lymph nodes by non-invasive and convenient blood sample detection

Method used

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  • miRNA biomarkers and detection kits for the diagnosis of cervical lymph node metastasis in papillary thyroid carcinoma
  • miRNA biomarkers and detection kits for the diagnosis of cervical lymph node metastasis in papillary thyroid carcinoma
  • miRNA biomarkers and detection kits for the diagnosis of cervical lymph node metastasis in papillary thyroid carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Screening of differential miRNAs in thyroid cancer tissue and lymph node tissue with and without lateral cervical lymph node metastasis

[0051] 1 Objects and Methods

[0052] 1.1 Specimen source

[0053] After obtaining the informed consent of the patients, 10 pairs of postoperative specimens from patients with papillary thyroid cancer who were pathologically confirmed to have cervical lateral lymph node metastasis from October 2019 to October 2020 in Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology were collected, including cancer tissue There were 10 specimens, and 10 positive lymph nodes with more than 50% infiltration of cancer lesions were confirmed by postoperative pathology; thyroid glands without lateral cervical lymph node metastasis confirmed by pathology from October 2019 to October 2020 in Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology were...

Embodiment 2

[0122] Example 2: Serological analysis of differential miRNAs in thyroid cancer tissue and lymph node tissue with and without lateral cervical lymph node metastasis

[0123] 1 Objects and Methods

[0124] 1.1 Specimen source

[0125] After obtaining the informed consent of the patients, 6 ml of fasting venous blood in the morning of 60 patients with papillary thyroid cancer with cervical lymph node metastasis confirmed by pathology from October 2019 to October 2020 in Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology was collected as The experimental group, and 120 patients with papillary thyroid cancer confirmed by pathology without lateral cervical lymph node metastasis, or papillary thyroid cancer with no abnormality in lateral cervical lymph nodes confirmed by preoperative examination and no metastasis in central lymph nodes confirmed by postoperative pathology Patients (hereinafter referred to as patients without latera...

Embodiment 3

[0180] Example 3: Receiver Operating Characteristic (ROC) Analysis

[0181]ROC curves were constructed to compare the diagnostic ability of 6 serum miRNAs in differentiating patients with lateral cervical lymph node metastases and controls without lateral cervical lymph node metastases. The area under the ROC curve (AUC) of the six miRNAs were: hsa-miR-146b, 0.876 (95% confidence interval: 0.825-0.926); hsa-miR-25-3p, 0.892 (95% confidence interval: 0.848-0.936) ; hsa-miR-296-5p, 0.926 (95% confidence interval: 0.891-0.961); hsa-miR-222-3p, 0.628 (95% confidence interval: 0.545-0.711); hsa-miR-21, 0.936 (95 % confidence interval: 0.904-0.968); hsa-miR-95, 0.742 (95% confidence interval: 0.665-0.819). At the optimal cut off values, the sensitivity and specificity of miRNAs were as follows: hsa-miR-146b, 65.0% and 92.5%, respectively; hsa-miR-25-3p, 100% and 67.5%, respectively; hsa-miR -296-5p, 100% and 76.7%, respectively; hsa-miR-222-3p, 76.7% and 45.0%, respectively; hsa-m...

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Abstract

The present invention proposes miRNA biomarkers and detection kits for the diagnosis of cervical lymph node metastasis of papillary thyroid carcinoma, the miRNAs are hsa-miR-146b, hsa-miR-25-3p, hsa-miR-296- 5p, hsa-miR-222-3p, hsa-miR-21 and hsa-miR-95 combination; detection kit comprising primers capable of amplifying said miRNA biomarkers and probes capable of detecting said miRNA biomarkers . The six miRNAs of the present invention are stably expressed in serum, and they are combined as biomarkers for the diagnosis of lateral cervical lymph node metastasis of thyroid cancer, and the sensitivity and specificity of the diagnosis are significantly higher than those of a single miRNA. The present invention uses serum miRNA to diagnose cervical lymph node metastasis in patients with papillary thyroid carcinoma. Compared with traditional pathological biopsy, the invention has less trauma and is suitable for preoperative diagnosis of patients who are difficult to perform lymph node puncture due to anatomical location and other reasons. , and can be repeated during patient follow-up.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a miRNA biomarker and a detection kit for diagnosing cervical lateral lymph node metastasis of papillary thyroid carcinoma. Background technique [0002] Thyroid cancer is one of the most common endocrine system malignancies. In the past 30 years, the incidence of thyroid cancer in the world has been increasing year by year. It has now become the fourth most common malignant tumor in women in my country, and has shown a rapid growth trend. Among them, papillary thyroid carcinoma (PTC) originates from thyroid follicular epithelial cells and is the most common type of thyroid cancer. About 27% of patients with papillary thyroid carcinoma are associated with lateral cervical lymph node metastasis. Lateral cervical lymph node metastasis is significantly associated with distant metastases to the patient's organs, the risk of death and the risk of recurrence. Once relapsed, 2 / 3 of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/113C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 李兴睿廖兴华李舒羽李汉宁王戈徐滔崔晓清张慧敏王君
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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