Method for simultaneously determining contents of eight index components in Miao medicine cynanchum auriculatum

A technology of eliminating mountains and indicators, applied in the field of medicine, can solve the problems of not fully and truly reflecting the quality of medicinal materials

Active Publication Date: 2021-07-06
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the chemical composition of traditional Chinese medicine has the characteristics of complexity and diversity, and it is also the material basis for its curative effect. If the active ingredie

Method used

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  • Method for simultaneously determining contents of eight index components in Miao medicine cynanchum auriculatum
  • Method for simultaneously determining contents of eight index components in Miao medicine cynanchum auriculatum
  • Method for simultaneously determining contents of eight index components in Miao medicine cynanchum auriculatum

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experiment example 1

[0014] 1 Instruments and materials

[0015] 1.1 Instrument

[0016] ACQUITY system ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer, including binary gradient pump, automatic sampler, column thermostat, triple quadrupole mass analyzer and Masslynx4.1 mass spectrometry workstation (Waters, USA), Allegra 64R low-temperature high-speed centrifuge (Beckman Coulter), ZH-2 vortex mixer (Tianjin Pharmacopoeia Standard Instrument Factory), AE240 1 / 100,000 electronic balance (Shanghai Mettler-Toledo Instrument Co., Ltd.) , FL-UP-YZ-20 ultrapure water instrument (Suzhou Feilu Environmental Protection Technology Co., Ltd.), KQ-250E ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0017] 1.2 Materials

[0018] Radix radix benzophenone (made by the laboratory), cudatin, pinoresin, apigenin (Chengdu Aifa Biotechnology Co., Ltd., batch numbers AF8071803, AF9052003, AF7061813, the purity is ≥98%), scopoletin, Syringic acid, ferulic acid...

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Abstract

The invention discloses a method for simultaneously determining the contents of eight index components in Miao medicine cynanchum auriculatum by a UPLC-ESI-MS (Ultra Performance Liquid Chromatography-ESI-Mass Spectrometry) method. According to the method, the contents of the eight index components in the Miao medicine cynanchum auriculatum are simultaneously determined by establishing the UPLC-ESI-MS method, wherein the eight index components comprise nodatin, pinoresinol, cynanchum bungeanum benzophenone, ferulic acid, gallic acid, scopoletin, syringic acid and chlorogenic acid. According to the method, an ACQUITYUPLCBEHC18 chromatographic column (2.1 mm*100 mm, 1.7 [mu]m) is adopted, a 0.1% formic acid aqueous solution and a 0.1% formic acid acetonitrile solution are adopted as a mobile phase, gradient elution is performed, the flow rate is 0.35 mL/min, the column temperature is 40 DEG C, and an electrospray ion source (ESI) and a multi-reaction ion monitoring (MRM) mode are adopted for detection. The method disclosed by the invention is simple, rapid, high in sensitivity and good in specificity, and can provide a reference basis for quality control of the cynanchum auriculatum medicinal material.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a UPLC-ESI-MS method for simultaneously determining the contents of eight index components in the Miao medicine Geshanxiao. Background technique [0002] Geshanxiao, also known as Feilaihe, Guoshanxiao, Baishouwu, etc., is the dry tuber root of Cynanchum auriculatum Royle ex Wight, a plant of the genus Cynanchum auriculatum Royle ex Wight, mainly produced in Hunan, Hubei, Sichuan and other places. The medicinal materials commonly used by ethnic minorities in the area, the roots are used as medicine by the folks. It has a long history of medicinal use in my country. It was first recorded in "Kaibao Materia Medica" in the Song Dynasty, and is now included in "Quality Standards for Traditional Chinese and Ethnic Medicinal Materials in Guizhou Province" (2003 edition ). Modern research shows that Geshanxiao mainly contains acetophenones, steroidal glycosides, organic acids, coumarins...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/88G01N30/86G01N30/34G01N30/06G01N30/14
CPCG01N30/02G01N30/72G01N30/88G01N30/8634G01N30/8679G01N30/34G01N30/06G01N30/14G01N2030/884
Inventor 孙佳秦兰刘春花陆苑潘洁郑林马雪李勇军
Owner GUIZHOU MEDICAL UNIV
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