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Coupling device, microscopic-optical tweezer single cell sorting system and sorting method

A technology of coupling device and sorting system, which is applied in the field of optical instruments, can solve the problems of single cell manipulation capture, separation and extraction, etc.

Pending Publication Date: 2021-07-20
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the observation of single cells by optical microscopy is limited to the observation of cell morphology, cell structure, fluorescence specificity, etc., and it is impossible to manipulate, capture, separate and extract single cells, which greatly limits the follow-up operations on the observed specific cells (such as cell culture, single cell sequencing, etc.)

Method used

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  • Coupling device, microscopic-optical tweezer single cell sorting system and sorting method
  • Coupling device, microscopic-optical tweezer single cell sorting system and sorting method
  • Coupling device, microscopic-optical tweezer single cell sorting system and sorting method

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Experimental program
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Effect test

Embodiment 1

[0052] Such as figure 1 Shown is a schematic diagram of the structure of the coupling device, including:

[0053]Top interface 11, filter frame 12 and dichroic mirror frame 15, bottom interface 14, first support plate 13 and second support plate 16, described top interface 11 and bottom interface 14 are placed in parallel, pass through the first support plate 13 is connected to the second support plate 16, the first support plate 13 is provided with a light hole, the dichroic frame 15 is installed on the second support plate 16, and the filter frame 12 is installed on the first support On the board 13, the top interface 11, the filter holder 12, the dichroic mirror holder 15 and the bottom structure 14 are all provided with concentric circular holes.

[0054] Preferably, the filter holder 12 and the dichroic mirror holder 15 are installed through a screw, and the mirror holder can be adjusted by adjusting the rotating screw.

[0055] Preferably, multiple sets of filter frame...

Embodiment 2

[0062] Such as figure 2 As shown, a fluorescence-optical tweezers single cell sorting system includes:

[0063] - fluorescence microscope, - coupling device, - optical tweezers capture module, - sorting module, - computer (not marked in the figure);

[0064] A fluorescence microscope, comprising an eyepiece imaging module 6, a detection and identification module 7, a microscope adapter plate 8, an objective microscopic module 9, and a microscope skeleton 10, the eyepiece imaging module 6, a detection and identification module 7, an objective microscopic module 9, and a microscope skeleton 10 is a commercial microscope, which can be disassembled and combined arbitrarily. The detection and identification module 7 is a fluorescence detection module, and the detection and identification module 7 is connected to the eyepiece imaging module 6. On the microscope frame 10, the top interface of the coupling device 1 is connected to the detection and identification module 7, the botto...

Embodiment 3

[0078] Such as image 3 As shown, a Raman-optical tweezers single cell sorting system includes:

[0079] -Raman microscope, -coupling device, -optical tweezers capture module, -sorting module, -computer (not marked in the figure);

[0080] Raman microscope, including eyepiece imaging module 6, detection and identification module 7, microscope adapter board 8, objective lens micro module 9, microscope skeleton 10, said eyepiece imaging module 6, detection and identification module 7, objective lens micro module 9, microscope The skeleton 10 is a commercial microscope, which can be disassembled and combined arbitrarily. The detection and identification module 7 is a Raman detection module, and the detection and identification module 7 is connected to the eyepiece imaging module 6. Installed on the microscope frame 10, the top interface of the coupling device 1 is connected to the detection and identification module 7, the bottom interface is connected to the microscope adapter ...

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Abstract

The invention discloses a coupling device and a microscopic-optical tweezer sorting system and sorting method. The coupling device comprises a top connector, an optical filter frame, a dichroscope frame, a bottom connector, a first supporting plate and a second supporting plate, and the top connector and the bottom connector are arranged in parallel and connected through the first supporting plate and the second supporting plate; the first supporting plate is provided with a light through hole, the dichroscope frame is installed on the second supporting plate, the optical filter frame is installed on the first supporting plate, and the top connector, the optical filter frame, the dichroscope frame and the bottom structure are all provided with concentric circular holes. An optical tweezer can be simply and quickly introduced into a commercial microscope through a coupling device, and meanwhile, a sorting system is added, so that the microscopic-optical tweezer sorting system and method are formed, the limitation that the commercial microscope is only used for observation is broken through, and multiple functions of single cell observation, capture operation and separation extraction are realized on one set of system.

Description

technical field [0001] The invention relates to the field of optical instruments, in particular to a coupling device, a micro-optical tweezers single cell sorting system and a sorting method thereof. Background technique [0002] Single-cell analysis can reveal the basic unit of life-the diversity and difference of cell material composition and physiological behavior, and is the mainstream cutting-edge technology of life analysis today. Single-cell analysis is the study of individual differences in cell populations, including cell size, growth rate, chemical composition (phospholipids, proteins, metabolites, DNA / RNA), and the causes and mechanisms of cell-to-cell differences. Its research content involves the fields of tumor biology, stem cells, microbiology, neurology and immunology. [0003] The biggest challenge in single-cell analysis lies in the small size of the cells, as well as the complex chemical composition and difficulty in trace amounts of components brought ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/00G02B21/32
CPCC12M41/36C12M47/04G02B21/32
Inventor 李远东阚凌雁任立辉籍月彤马波徐健
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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