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Method for simulating metabolism of trimethylamine by intestinal flora

A technology of intestinal flora and trimethylamine, applied in the field of intestinal metabolite detection, can solve the problems of low detection sensitivity, increase the burden of detection personnel, increase the difficulty of patient sampling, etc., to achieve easy detection and avoid kinetic deviations. Effect

Pending Publication Date: 2021-07-20
HANGZHOU HALO MEDICAL TECH CO LTD +2
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  • Description
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Problems solved by technology

[0005] 1. It is necessary to directly detect fecal TMA and be forced to weigh it, which increases the difficulty of patient sampling and increases the burden on testing personnel. At present, the equipment cannot realize automatic weighing and dilution
[0006] 2. Feces need to be detected directly, even with mass spectrometry, the detection sensitivity is not high
[0007] 3. TMA metabolized by bacteria will affect the results of feces due to intestinal absorption rate, feces retention time, etc.

Method used

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  • Method for simulating metabolism of trimethylamine by intestinal flora
  • Method for simulating metabolism of trimethylamine by intestinal flora

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Embodiment Construction

[0037] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0038] according to Figure 1-2 As shown, the embodiment of the present invention provides a method for simulating the metabolism of trimethylamine by intestinal flora, comprising the following steps: diluting the flora sample, and injecting the flora sample into the culture medium;

[0039] Fermentation is carried out in the medium, and NaOH is added to the medium after the fermentation is completed to free trimethylamine, and the trimethylamine produced in the medium is sampled;

[0040] The sampled trimethylamine is tested, and the disease risk level is obtained according to the obtained test results.

[0041] The flora sample is a feces sample; after the feces ...

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Abstract

The invention provides a method for simulating metabolism of trimethylamine by intestinal flora. The method is used for achieving the purposes of simplifying the detection process and improving the detection accuracy. The method comprises the following steps: diluting a flora sample, and injecting the flora sample into a culture medium; performing fermentation in a culture medium, adding NaOH into the culture medium after fermentation is finished, dissociating trimethylamine, sampling trimethylamine generated in the culture medium, detecting the sampled trimethylamine, and obtaining the disease risk grade according to the obtained detection result; in-vitro simulated culture is carried out on intestinal flora through an indirect method, the amount of trimethylamine produced by metabolic choline and L-carnitine of the intestinal flora is detected, and the risk of suffering from cardiovascular diseases when a patient takes food with high relative content of choline and carnitine is further evaluated.

Description

technical field [0001] The invention relates to the technical field of intestinal metabolite detection, in particular to a method for simulating the metabolism of trimethylamine by intestinal flora. Background technique [0002] At present, choline substances from food, such as L-carnitine, choline, etc., can be metabolized by intestinal bacteria into trimethylamine (TMA, Trimethylamine), and trimethylamine passes through liver FMO kinase, that is, flavin monooxygenase (English :Flavin-containing monooxygenase) into trimethylamine oxide TMAO (Trimetlylamineoxide), TMAO can promote the inflammation of vascular endothelium and the downstream plate formation. Those with the highest TMAO had two to three times higher rates of adverse cardiovascular disease outcomes over three years than those with the lowest blood levels. [0003] Small-molecule inhibitors targeting TMA production have been born, and have attracted the layout of pharmaceutical giants such as AstraZeneca and Pfi...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 陈洁莹王婷婷朱立颖彭水强王欣李降龙
Owner HANGZHOU HALO MEDICAL TECH CO LTD
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