Mesenchymal stem cell exosome-AM1241 complex and application of mesenchymal stem cell exosome-AM1241 complex in treatment of Alzheimer's disease

A technology of Alzheimer's disease and mesenchymal stem cells, which is applied in the field of biomedicine to achieve the effect of improving the disease

Active Publication Date: 2021-07-30
SHANGHAI TONGJI HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the study of the effect of AM1241 on the treatment of Alzheimer's disease, and there is no

Method used

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  • Mesenchymal stem cell exosome-AM1241 complex and application of mesenchymal stem cell exosome-AM1241 complex in treatment of Alzheimer's disease
  • Mesenchymal stem cell exosome-AM1241 complex and application of mesenchymal stem cell exosome-AM1241 complex in treatment of Alzheimer's disease
  • Mesenchymal stem cell exosome-AM1241 complex and application of mesenchymal stem cell exosome-AM1241 complex in treatment of Alzheimer's disease

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Embodiment 1

[0027] The research route of the present invention is:

[0028] (1) Construct the Exo-AM1241 nano-drug system, identify the nano-drug system by transmission electron microscopy and immunoblotting, and compare the brain-entry efficiency of the nano-drug system and AM1241 alone by high-performance liquid chromatography;

[0029] (2) Use Exo-AM1241 to treat Alzheimer's disease model mice, and analyze its effect on the learning and memory ability of animals;

[0030] (3) The inhibitory effect of Exo-AM1241 on amyloid deposition and neuronal apoptosis was analyzed by means of immunofluorescence and western blotting;

[0031] (4) RT-qPCR was used to explore the potential mechanism of Exo-AM1241 / exosome / AM1241 in the treatment of Alzheimer's disease.

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Abstract

The invention relates to a mesenchymal stem cell exosome-AM1241 complex and an application of the mesenchymal stem cell exosome-AM1241 complex in treatment of Alzheimer's disease. It is found for the first time that the exosome-loaded CB2 receptor selective agonist AM1241 can effectively improve the Alzheimer's disease, and animal experiments show that an Exo-AM1241 nano-drug system can basically recover the learning and memory ability of an Alzheimer's disease model mouse to a normal level; it is found for the first time that AM1241 can effectively improve the Alzheimer's disease; the invention also proves that the improvement effect of the mesenchymal stem cell exosome-AM1241 complex on the Alzheimer's disease is obviously better than that of the single AM1241 or exosome, and reveals that the effects and molecular mechanisms of the mesenchymal stem cell exosome-AM1241 complex, the single AM1241 or exosome on improving the Alzheimer's disease are obviously different.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a mesenchymal stem cell exosome-AM1241 complex and its application in the treatment of Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a chronic degenerative disease of the central nervous system. Patients will mainly have clinical symptoms such as progressive memory loss, cognitive dysfunction, personality changes and language barriers, which will seriously affect the patient's mental health. Social, professional and life functions. Alzheimer's disease not only brings a heavy burden to patients and their families, but also a serious social and economic problem. However, the pathogenesis of AD is still unclear, and most of the existing clinical drugs can only delay the progression of dementia, but cannot fundamentally treat it. Therefore, it is urgent to find new therapeutic targets and new drug carriers / drug new dosage forms , so many researc...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K31/454A61K47/69A61P25/28
CPCA61K35/28A61K31/454A61K47/6901A61P25/28A61K2300/00
Inventor 程黎明朱融融朱颜菁黄蕊奇
Owner SHANGHAI TONGJI HOSPITAL
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