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Kit for detecting coronavirus antibody and detection method of coronavirus antibody

A coronavirus and detection method technology, applied in the field of coronavirus antibody detection and coronavirus antibody detection kits, can solve the problems of complex operation, high requirements on the biosafety level of coronavirus antibodies, and low detection throughput. High throughput, large-scale detection, and simple composition

Inactive Publication Date: 2021-08-03
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kit for detecting coronavirus antibodies and a detection method for coronavirus antibodies, aiming to solve the problems of high biosafety level, complicated operation, and general detection problems existing in existing coronavirus antibody detection technologies. low volume technical issues

Method used

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  • Kit for detecting coronavirus antibody and detection method of coronavirus antibody
  • Kit for detecting coronavirus antibody and detection method of coronavirus antibody
  • Kit for detecting coronavirus antibody and detection method of coronavirus antibody

Examples

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Embodiment

[0048] This example provides a method for detecting novel coronavirus antibodies and corresponding kits using the stock solution of the novel coronavirus vaccine. In the kit, the main component of the capture component is magnetic particles (magnetic beads for short) coated with the stock solution of the new coronavirus vaccine, and the main component of the detection component is the stock solution of the new coronavirus vaccine labeled with alkaline phosphatase.

[0049] 1.1 Preparation of reagents

[0050] 1.1.1 Preparation process of capture components

[0051] The new coronavirus inactivated vaccine stock solution (China Biotechnology Co., Ltd.) was coated on the surface of magnetic beads (ThermoFisher). The corresponding wrapping method is described in detail below:

[0052] First, the stock solution of the novel coronavirus inactivated vaccine is pretreated, and the protective components in its buffer matrix are removed by dialysis. Coating is carried out according t...

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Abstract

The invention belongs to the technical field of biological detection, and particularly relates to a kit for detecting a coronavirus antibody and a detection method of the coronavirus antibody. The kit provided by the invention can be used for detecting the coronavirus antibody, the coronavirus antibody in a to-be-detected sample is respectively combined with a coronavirus vaccine stock solution in a capture component and a coronavirus vaccine stock solution in a detection component, and then the detection result is judged according to the luminous intensity; therefore, the detection of the coronavirus antibody in the to-be-detected sample is realized. The detection result can be quickly obtained without performing in a BSL-3 laboratory in the detection process, and the method has the advantages of simplicity and convenience in operation, high specificity and high detection flux.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit for detecting coronavirus antibodies, the use of coronavirus vaccine stock solution in preparing detection reagents for detecting coronavirus antibodies, and a method for detecting coronavirus antibodies. Background technique [0002] Coronavirus (Coronavirus) is a large class of viruses that widely exist in nature, and is an RNA virus with a diameter of about 80-120 nm. At present, seven kinds of coronaviruses that can infect humans have been found, namely human coronavirus 229E (HCoV-229E), HCoV-OC43, SARS virus (SARS-CoV), HCoV-NL63, HCoV-HKU1, MERS virus (MERS- CoV) and novel coronavirus (SARS-CoV-2). [0003] Corona Virus Disease 2019 (COVID-19) is an acute respiratory infectious disease caused by a novel coronavirus (SARS-CoV-2). Public health events, and became one of the deadliest epidemics in human history. [0004] The new coronavirus contains four...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/58G01N33/543
CPCG01N33/56983G01N33/588G01N33/582G01N33/583G01N33/54306G01N2333/165G01N2469/20
Inventor 杨晓明张云涛夏小凯王瑜新程鲁向程天龄解传芬李可詹成雄刘君君于丽娜
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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