57results about How to "Does not affect the detection effect" patented technology

The invention discloses a fixed-position human behavior analysis method, and the method comprises the following steps: 1), carrying out the HOG feature extraction of a human body detection target or scanning window; 2), carrying out the CSS feature extraction of the human body detection target or scanning window; 3), extracting the HOG and CSS features, inputting a trained SVM, and judging whether the inputted trained SVM is a human body or not: carrying out SVM human body detection if the inputted trained SVM is the human body; 4), carrying out the CNN human body second confirmation of a detected human body image; 5), marking key parts in the human body image with landmark key points, connecting the landmark key point, forming integrated description of the human body or face, and carrying out shape regression; 6), carrying out the MHCRF behavior recognition of the series of obtained landmark key points related with a target attitude through the relative position of the obtained landmark key points. The method has the advantages of invariance and normalization, and is especially suitable for the detection of the human body in the image.

The present invention relates to the preparation of a cell slide assembly for a CBA kit for detecting MOG-IgG, the CBA detection kit and its application. The preparation method of the cell slide assembly is as follows: S1: construction of MOG plasmid; S2: overexpression of MOG gene Lentiviral packaging; S3: Construction of a cell line (MOG-293T cell line) overexpressing the MOG gene; S4: Preparation of cell slide components. The kit includes cell slide assembly, anti-human IgG-FITC secondary antibody, blocking solution, PBS and mounting medium; the kit can be used for detection of MOG autoimmune IgG antibody in clinical samples. The kit of the invention has the advantages of simple operation, strong detection specificity, high sensitivity, good repeatability of experimental results, can be popularized and applied, and can be used for detection of MOG autoimmune IgG antibody in clinical samples.

The present invention discloses a bill thickness detecting device arranged in an ATM banknote processing mechanism. The device comprises a conveying roller, an upper infrared distance measurement sensor, a lower infrared distance measurement sensor, and a controller. The conveying roller provides power to allow a bill to move along a bill transport path. The upper infrared distance measurement sensor and the lower infrared distance measurement sensor are symmetrically arranged at two sides of the bill transport path and are connected to the controller and transport distance data detected in real time to the controller. The controller is internally provided with a data collection module, a data analysis module, and a data processing module. The data collection module receives measurement data in real time. The data analysis module analyzes the measurement data. The data processing module receives the data information analyzed by the data analysis module and judges whether the bill is qualified or not. According to the technical scheme, comprehensive bill thickness data can be obtained, whether the bill is qualified or not can be comprehensively evaluated, the bill conveying speed can be raised, the detection efficiency is improved, and the detection effect is not affected.

The invention discloses a kit for quantitatively detecting PML/RARalpha mRNA level. The kit comprises a standard product for manufacturing a standard curve, an internal reference gene real-time quantitative PCR system and at least one of three real-time quantitative PCR systems: L-type PML/RARalpha real-time quantitative PCR system, S-type PML/RARalpha real-time quantitative PCR system and V-type PML/RARalpha real-time quantitative PCR system. The kit can accurately, rapidly and quantitatively detect the PML/RARalpha mRNA level, is used for diagnosis of acute promyelocytic leukemia and monitoring of minimal residual diseases during treatment, and provides important molecular basis for confirmed diagnosis of clinic diseases, determination of treatment proposals, curative effect evaluation and prognosis.

The invention discloses a motion direction based abnormal behavior detection method in monitoring video, which solves a problem that a video abnormal behavior is difficult to be detected accurately through analyzing the motion direction. The abnormal behavior detection method mainly comprises the following three parts: preliminary video object acquisition, video object optimization and video object abnormal behavior detection. In the first part, original video frames and background video frames are processed, and a preliminary video object is acquired by using an improved background subtraction method; in the second part, preliminary video object pixels with the moving speed being within a noise boundary threshold are screened, and the accurate position of the video object is acquired; and in the third part, the motion direction of the video object pixels is calculated, and a motion direction entropy is calculated so as to judge whether a behavior is abnormal or not. The abnormal behavior detection method simulates artificial marking, can detect most of the abnormal situations in time and has good real-time performance and effectiveness.

The invention discloses a financial document image segmentation and correction method based on a mobile terminal. The method comprises the following steps: (1) preprocessing an image; (2) performing foreground and background segmentation on the image; (3) carrying out contour detection on the segmented image; (4) removing redundant contours; (5) carrying out straight line fitting on the document contour to obtain four straight lines, and then solving four intersection points; and (6) correcting the position of the document to obtain a standard rectangle. According to the financial document image segmentation and correction method based on the mobile terminal, the financial document image is accurately segmented under different illumination intensities, and the whole algorithm has the advantages of being simple, high in accuracy, good in robustness and high in positioning speed.

The invention discloses a method for separation, enrichment and detection of Cu and Zn in food. The method is as below: co-precipitating Cu and Zn in a food sample with 2,9 -dimethyl-4,7-diphenyl-1,10- phenanthroline disodium sulfonic acid (BCP); and then determining the content of Cu and Zn by using a flame atomic absorption spectrometry (FAAS). The method has the advantages of simple process, high detection and sedimentation speed and low cost; BCP is used as a co-precipitation agent, and no vector element is needed. Compared with the prior literature art, the method has the advantages of low detection limit and high degree of accuracy.

The invention discloses a fluorescence compound for detecting trace cyanogens ions as well as a preparation method and application of the fluorescence compound, and belongs to the field of fluorescence chemical sensors. The fluorescence compound is called as TPP-CN for short. The preparation method comprises the following steps: firstly, dissolving TPP into a good solvent 1, adding phenylboronic acid, a catalyst and alkali salt, performing a sufficient reaction at certain temperature so as to obtain TPP-CHO; dissolving TPP-CHO into a good solvent 2, adding malononitrile, performing a sufficient reaction at certain temperature, and purifying, thereby obtaining TPP-CN. The TPP-CN has high-sensitivity specific fluorescence response to cyanogens ions in a water body, dual response of fluorescence intensity quenching and color 'blue shift' can be resulted, and moreover the response is very rapid to implement.

The invention discloses a Zr < 4 + >-induced metal organic gel fluorescent switch sensing material and a preparation method and application thereof, and the method comprises the following steps: A1, transferring DMF and H2O into a reaction vessel, and then fully shaking until the DMF and H2O are completely mixed, wherein the volume ratio of DMF to H2O is 7: 3; A2, adding BTC into the reaction container, wherein the reaction container is subjected to ultrasonic treatment for 20 seconds until the ligand BTC in the system is completely dissolved, and then adding ZrCl4 into the mixed solvent, wherein the molar ratio of ZrCl4 to BTC is 1: 4; and A3, putting the reaction system into an ultrasonic instrument, and carrying out ultrasonic treatment at 80 DEG C for 30 minutes to form white columnargel. According to the constructed novel fluorescence switch sensing platform, ARG can be selectively detected through a charge transfer effect, and CrO4 < 2-> in water can be rapidly detected and removed through energy resonance transfer and a chemical adsorption effect.

A pedestrian detection method provided by the invention comprises the following steps: S100, training SVM (Support Vector Machine) classifiers, and training the two SVM classifiers by taking a human head and two legs as positive samples and taking a pedestrian background as a negative sample; S200, constructing an image pyramid by using the to-be-detected image; S300, sliding the window, and intercepting a target window on each scale of the image pyramid; S400, extracting HOG features from the target window; S500, sending the HOG features extracted in the step S400 into an SVM classifier, andjudging whether the target window is the head of a pedestrian, the two legs of the pedestrian or the pedestrian background in a classified mode; And S600, combining pedestrians according to the head and leg results detected in the step S500, and judging whether the to-be-detected image contains the pedestrians or not. Therefore, the pedestrian detection rate is improved by detecting the combination strategy of the human head and the two legs, and the problem that the pedestrian detection rate is not high is solved through the pedestrian detection and recognition method based on the SVM and theHOG.

The invention discloses a fluorescent probe for detecting mercaptoamino-acid based on naphthalimide indole and a preparation method of the fluorescent probe. The structure of the fluorescent probe isshown as follows (please see the specifications for the structure). The fluorescent probe is used for detecting cysteine, homocysteine and glutathione, and is good in selectivity, high in anti-interference capability and high in response speed; and meanwhile, the fluorescent probe has long-wavelength transmission and large stroke shift in the detecting process, detecting sensitivity can be improved, and small background interference can be achieved. The fluorescent probe can be used for detecting the mercaptoamino-acid in biological samples and cells.

The invention discloses a copper ion detecting reagent and a preparation method thereof, a piece of test paper and a preparation method thereof, and application in copper ion detection. The copper iondetecting reagent is 1-(2-pyridylazo)-2-naphthol-6-sulfonic acid soluble salt aqueous solution. The test paper comprises a base material and a functional layer deposited on the outer surface of the base material; the functional layer is formed by alternately deposition of a positively charged organic compound layer and 1-(2-pyridylazo)-2-naphthol-6-sulfonic acid soluble salt layer. The structuralformula of the 1-(2-pyridylazo)-2-naphthol-6-sulfonic acid soluble salt is shown as a formula (I); and in the formula, M(xor) is selected from alkali metal ion or ammonium ion and the positively charged organic compound is selected from a positively charged organic small molecule or cationic polyelectrolyte. With the copper ion detecting reagent and the copper ion detecting test paper, the copperions in the water body can be detected conveniently and flexibly by naked eyes; the detection sensitivity and the selectivity are high; and no instrument equipment is needed. The formula (I) is shownin the specification.

A pedestrian positioning and track acquisition method based on a camera video stream is characterized by sequentially comprising two steps: 1, a feature extraction and detection algorithm of an indoortarget person; wherein the pedestrian detection module is used for performing pedestrian detection on each frame of image of a camera video stream; 2, a pedestrian position information and a moving track acquisition algorithm thereof; wherein the algorithm is used for acquiring position information and moving tracks of pedestrians detected in each frame of image of a camera video stream. According to the method, the space models of the indoor camera and the pedestrian are established, the related models are established from the vertical section and the horizontal section of the pedestrian respectively, the established models are simple and efficient, the required calculation amount is small, the accuracy of the acquired position information is high, the calculation force can be saved, thetime consumption is reduced, and the real-time performance is ensured.

The invention discloses a gas detection device for underground detection, and relates to the technical field of gas detection. The device comprises a mounting plate, wherein the surface of the mounting plate is connected with a telescopic rod, and the upper surface of the mounting plate is connected with a gas detector; the bottom of an L-shaped plate is connected with a first pulley, a traction rope is wound on the first pulley, one end of the traction rope is fixedly connected with a second pulley, and the other end of the traction rope is fixedly connected with a third pulley; a sliding rope is wound around the third pulley, one end of the sliding rope is fixed to the bottom of the L-shaped plate, and the other end of the sliding rope is slidably connected with the second pulley; the lower end of the third pulley is connected with a floating plate floating on the water surface, and the surface of the floating plate is connected with sliding rods; the two ends of the two sliding rods are in sliding connection with probe mounting plates; and a gas detection probe is mounted on the upper surface of the probe mounting plate. Through cooperation of the first pulley, the second pulley, the third pulley, the traction rope and the sliding rope, the detection efficiency is improved, and the service life is prolonged.

The invention discloses a universal elisa plate detergent for increasing the signal-to-noise ratio of an ELISA (Enzyme Linked Immunosorbent Assay) kit and a use method of the universal elisa plate detergent and belongs to the technical field of biologics. The universal elisa plate detergent for increasing the signal-to-noise ratio of the ELISA kit comprises 0.1-0.35M NaCl, 0.05-0.1%vol triton X-100, 3-6g/L N-lauroyl sarcosine sodium and the balance of a 0.01M phosphate buffer solution, wherein the pH value of the phosphate buffer solution is 7.2-7.4. Due to addition of SDS (Sodium Dodecyl Sulfonate) and Triton-X100 with relatively good cleaning properties into the detergent, the ionic strength of the detergent is improved, the nonspecific mutual action of molecules is effectively controlled, furthermore a low background and a high signal-to-noise ratio of ELISA experiment results are achieved, the detection sensitivity and accuracy are improved, and the universal elisa plate detergentis relatively good in universality and very good in popularization application potential.

The invention discloses preparation of an AQPs high-expression brain region slice detection assembly with abundant capillaries and vascular endothelium required by a human AQPs autoantibody detectionkit, an indirect immunofluorescence kit and application thereof. The preparation method of the microvascular and vascular endothelium-rich AQPs high-expression brain region slice detection assembly comprises the following steps: obtaining rat brain tissues; preparing a specific brain area tissue slice; and detection assembly preparation. The kit comprises the microvascular and vascular endothelium-rich AQPs high-expression brain region slice detection assembly, a confining liquid, PBST, a mounting medium and a fluorescent secondary antibody. The kit can be used for detecting anti-AQP4, AQP1 and other AQPs autoantibodies in clinical serum and cerebrospinal fluid samples. The kit is easy to operate, high in sensitivity, strong in staining form specificity, simple in result interpretation, accurate and reliable, and capable of supplementing the detection capability on AQP1 and other AQPs antibodies on the basis of ensuring that the detection on the AQP4 spatial conformational antibody isnot lower than the specificity and sensitivity of other means and meeting the requirements of auxiliary clinical diagnosis.

A safety explosion-proof apparatus for continuously monitoring material fermentation and an operating method thereof are provided. In large-scale biogas fermentation process, a fermentation tank is made from an opaque material, such as concrete, and is completely sealed and dark, and providing a probe or a viewer to monitor materials in real time is required; a mark may be left on the surface of a monitoring device by scum floating up during stirring, and observation is blocked; fermentation produced gas contains flammable combustible gas and little device-corrosive gas, resulting in influences on monitoring stability. The safety explosion-proof apparatus comprises a fermentation tank, a seal cover and a heating tape; a pressure valve is mounted at the left top of the fermentation tank and is connected with a water incoming pipe, the water incoming pipe is connected with a nozzle, a glass seal hood is mounted at the upper left of the fermentation tank, a heating fan and a lighting probe are fixed in the glass seal hood, the lighting probe is connected with an external conductor, the external conductor is connected with a display, and an alarm is mounted on the seal cover and is connected with a pressure sensor. The safety explosion-proof apparatus is simple in structure, safe and handy.

The invention discloses a kit for detecting rhinoviruses, respiratory syncytial viruses and parainfluenza viruses by a micro-fluidic chip and an application method of the kit. The kit comprises the multi-flux microfluidic nucleic acid detection chip, a preloaded dry powder testing agent and a positive quality control. The multi-flux microfluidic nucleic acid detection chip is used for controllingflow passages actively. The preloaded dry powder testing agent comprises a primer for specific conserved sequences of the rhinoviruses, the respiratory syncytial viruses and the parainfluenza virusesand a TagMan fluorescence probe. The kit has the advantages that the micro-fluidic chip technology is applied, all operations are completed by instruments after sample loading, convenience and simplicity in operation and high speed are realized, detection can be completed within 30-60 minutes, and pollution is avoided; the TagMan fluorescence probe PCR technology is applied to detect the rhinoviruses, the respiratory syncytial viruses and the parainfluenza viruses; designed primer and probe sequences are quite conservative in genes of the rhinoviruses, the respiratory syncytial viruses and theparainfluenza viruses, high specificity is achieved, detection results can be acquired within two hours, and sensitivity can reach 10copies/microliter.

The invention discloses a detection two-wheel robot utilizing a two-wheel differential chassis to steer and belongs to the technical field of robots. The detection two-wheel robot comprises the chassis, a moving component is arranged at the lower end of the chassis, the upper part of the moving component is mounted with the chassis through a mounting plate, the upper part of the chassis is connected with a movable shaft disc, and a positioning device is arranged between the chassis and the movable shaft disc. According to the detection two-wheeled robot utilizing the two-wheeled differential chassis to steer, the stability is improved, meanwhile, the energy loss is reduced, the steering speed is increased, the use convenience is met, the adaptability of the whole robot is improved, the adjustability of the robot is improved, the use requirement is met, multi-angle adjustment is facilitated, the stability is high, the sensitivity is greatly improved, the working efficiency is greatly improved, the detection efficiency is improved, detection can be carried out for various environments, danger caused by manual detection is avoided, the use safety is improved, and the personal safety is guaranteed while the detection effect is not affected.