Kit for quantitatively detecting AML1/ETO mRNA level
A quantitative detection and kit technology, which is applied in the field of kits for quantitative detection of AML1/ETO mRNA levels, achieves the effects of strong practicability, cost reduction and cost reduction
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] Embodiment 1, the preparation of the kit of quantitative detection AML1 / ETO mRNA level
[0025] 1. Design of primers and probes in the kit for quantitative detection of AML1 / ETO mRNA levels
[0026] AML1 / ETO real-time quantitative PCR system is as follows:
[0027] (1) Upstream primer (located at exon 5 of AML1): 5'-CACCTACCACAGAGCCATCAAA-3' (sequence 1 in the sequence listing), with a final concentration of 0.3 μM;
[0028] (2) Downstream primer (located at ETO exon 2): 5'-ATCCACAGGTGAGTCTGGCATT-3' (sequence 2 in the sequence listing), the final concentration is 0.3 μM;
[0029] (3) TaqMan probe (located in ETO exon 2): 5'-AACCTCGAAATCGTACTGAGAAGCACTCCA-3' (sequence 3 in the sequence listing), the final concentration is 0.2 μM;
[0030] (4) Master Mix for fluorescent PCR (purchased from ABI, USA).
[0031] The internal reference gene real-time quantitative PCR system is as follows:
[0032] (1) Upstream primer (located in ABL exon 2):
[0033] 5'-TGGAGATAACACTCTAA...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com