Universal elisa plate detergent for increasing signal-to-noise ratio of ELISA (Enzyme Linked Immunosorbent Assay) kit and use method of universal elisa plate detergent

Abstract

The invention discloses a universal elisa plate detergent for increasing the signal-to-noise ratio of an ELISA (Enzyme Linked Immunosorbent Assay) kit and a use method of the universal elisa plate detergent and belongs to the technical field of biologics. The universal elisa plate detergent for increasing the signal-to-noise ratio of the ELISA kit comprises 0.1-0.35M NaCl, 0.05-0.1%vol triton X-100, 3-6g / L N-lauroyl sarcosine sodium and the balance of a 0.01M phosphate buffer solution, wherein the pH value of the phosphate buffer solution is 7.2-7.4. Due to addition of SDS (Sodium Dodecyl Sulfonate) and Triton-X100 with relatively good cleaning properties into the detergent, the ionic strength of the detergent is improved, the nonspecific mutual action of molecules is effectively controlled, furthermore a low background and a high signal-to-noise ratio of ELISA experiment results are achieved, the detection sensitivity and accuracy are improved, and the universal elisa plate detergentis relatively good in universality and very good in popularization application potential.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a general-purpose microplate washing solution capable of effectively controlling the non-specific binding between proteins during the operation of an ELISA kit, reducing the background and improving the signal-to-noise ratio. Background technique [0002] ELISA (enzyme linked immunosorbent assay), also known as enzyme-linked immunosorbent assay, refers to coating a known antigen or antibody on an enzyme-linked plate to test whether there is an antibody or antigen that can specifically bind to it in the sample to be tested . ELISA is widely used in scientific research and clinical testing due to its convenience, rapidity and sensitivity. The initial ELISA methods mainly include direct method and indirect method. According to the basic principle of antigen-antibody reaction, people have successively derived sandwich method and blocking method according to the needs of research, and deve...

AI Technical Summary

Problems solved by technology

However, it was found in the research that washing the microtiter plate with PBST, for many ELISA experiments, especially the detection of antigens or antibodies in serum, the background is high, and the OD value of negative samples is even as high as 0.2 to 0.5 (the smaller the dilution factor of the serum, the lower the background The higher), it is difficult to distinguish from the low-concentration detection target in the sample to be tested, which reduces the sensitivity and accuracy of detection

In addition, in the research and testing, it was found that some commercial clinical ELISA detection kits or ELISA detection reagents for research also have high background, and their cut off value (judgment threshold) is even as high as 0.3-0.4