Method for separation, enrichment and detection of Cu and Zn in food

Abstract

The invention discloses a method for separation, enrichment and detection of Cu and Zn in food. The method is as below: co-precipitating Cu and Zn in a food sample with 2,9 -dimethyl-4,7-diphenyl-1,10- phenanthroline disodium sulfonic acid (BCP); and then determining the content of Cu and Zn by using a flame atomic absorption spectrometry (FAAS). The method has the advantages of simple process, high detection and sedimentation speed and low cost; BCP is used as a co-precipitation agent, and no vector element is needed. Compared with the prior literature art, the method has the advantages of low detection limit and high degree of accuracy.

Description

technical field

[0001] The invention belongs to the detection field of Cu and Zn, in particular to a separation, enrichment and detection method of Cu and Zn in food. Background technique

[0002] Generally speaking, part of the heavy metal elements in food comes from the bioaccumulation of animals and plants; the other part comes from food production and processing, tissue cells appear structural and functional damage, and seriously affect human life in the form of chronic poisoning. Safety. Heavy metal pollution in food is one of the serious problems faced by human beings, and it is particularly important to carry out effective detection of heavy metal pollution in food.

[0003] Copper is an indispensable trace element in the human body and plays a very important role in maintaining normal physiological functions of the human body. At the same time, the lack of pigment in the body will reduce the pigment oxidase in the cells, and the vitality will decrease, so that peop...

Examples

Embodiment 1

[0018] Embodiment 1: the method for the separation, enrichment and detection of Cu and Zn in food rice (set 3 repeated tests), the specific content is as follows:

[0019] (1) Place the rice in an oven and bake it at 80°C for 7 hours, then crush and grind it to below 80 mesh;

[0020] (2) Weigh 0.50g of rice flour and place it in a porcelain crucible, add 1ml of nitric acid solution (volume ratio of nitric acid to water: 1:1), then place it on an electric heating plate, heat it on a low heat to carbonize it, and wait until the smoke is exhausted After that, it shows that it has been completely carbonized. After cooling, place it in a muffle furnace, heat it at 500°C for 2 hours, take it out and cool it; The water volume ratio is 1:1), then put it on the electric heating plate and evaporate it to dryness on low heat, then put it into the muffle furnace and heat it at 500°C for 1 hour, take it out, at this time, there is white ash in the pot, indicating that it has been complete...

Embodiment 2

[0025] Embodiment 2: the method for the separation, enrichment and detection of Cu and Zn in food rice (set 3 repeated tests), the specific content is as follows:

[0026] (1) Place the rice in an oven and bake it at 80°C for 6 hours, then crush and grind it to below 80 mesh;

[0027] (2) Weigh 0.50g of rice flour and place it in a porcelain crucible, add 1mL of nitric acid solution (volume ratio of nitric acid to water: 1:1), then place it on a heating plate, heat it on a low heat to carbonize it, and wait until the smoke exhausts After that, it shows that it has been completely carbonized. After cooling, place it in a muffle furnace, heat it at 500°C for 2 hours, take it out and cool it; add HNO dropwise 3 Solution (volume ratio of nitric acid to water: 1:1) 2.0mL was placed on electric heating and slightly boiled to dissolve, and the digestion solution was obtained;

[0028] (3) Take 1mL of rice digestion solution, adjust its pH to 10 with NaOH solution, then add 5mg of pr...

Embodiment 3

[0029] Embodiment 3: the method for the separation, enrichment and detection of Cu and Zn in food rice (set 3 repeated tests), the specific content is as follows:

[0030] (1) Put the mung bean sample in an oven at 80°C for 10 hours, and grind it to less than 80 meshes;

[0031] (2) Weigh 0.50g mung bean powder and place it in a porcelain crucible, add 1mL nitric acid solution (volume ratio of nitric acid to water: 1:1), then place it on a heating plate, heat it on a low heat to carbonize it, and wait until the smoke is exhausted After that, it shows that it has been completely carbonized. After cooling, place it in a muffle furnace, heat it at 500°C for 2 hours, take it out and cool it; The water volume ratio is 1:1), then put it on the electric heating plate and evaporate it to dryness on low heat, then put it into the muffle furnace and heat it at 500°C for 1 hour, take it out, at this time, there is white ash in the pot, indicating that it has been completely ashed, wait f...

technical field

[0001] The invention belongs to the detection field of Cu and Zn, in particular to a separation, enrichment and detection method of Cu and Zn in food. Background technique

[0002] Generally speaking, part of the heavy metal elements in food comes from the bioaccumulation of animals and plants; the other part comes from food production and processing, tissue cells appear structural and functional damage, and seriously affect human life in the form of chronic poisoning. Safety. Heavy metal pollution in food is one of the serious problems faced by human beings, and it is particularly important to carry out effective detection of heavy metal pollution in food.

[0003] Copper is an indispensable trace element in the human body and plays a very important role in maintaining normal physiological functions of the human body. At the same time, the lack of pigment in the body will reduce the pigment oxidase in the cells, and the vitality will decrease, so that peop...

Examples

Embodiment 1

[0018] Embodiment 1: the method for the separation, enrichment and detection of Cu and Zn in food rice (set 3 repeated tests), the specific content is as follows:

[0019] (1) Place the rice in an oven and bake it at 80°C for 7 hours, then crush and grind it to below 80 mesh;

[0020] (2) Weigh 0.50g of rice flour and place it in a porcelain crucible, add 1ml of nitric acid solution (volume ratio of nitric acid to water: 1:1), then place it on an electric heating plate, heat it on a low heat to carbonize it, and wait until the smoke is exhausted After that, it shows that it has been completely carbonized. After cooling, place it in a muffle furnace, heat it at 500°C for 2 hours, take it out and cool it; The water volume ratio is 1:1), then put it on the electric heating plate and evaporate it to dryness on low heat, then put it into the muffle furnace and heat it at 500°C for 1 hour, take it out, at this time, there is white ash in the pot, indicating that it has been complete...

Embodiment 2

[0025] Embodiment 2: the method for the separation, enrichment and detection of Cu and Zn in food rice (set 3 repeated tests), the specific content is as follows:

[0026] (1) Place the rice in an oven and bake it at 80°C for 6 hours, then crush and grind it to below 80 mesh;

[0027] (2) Weigh 0.50g of rice flour and place it in a porcelain crucible, add 1mL of nitric acid solution (volume ratio of nitric acid to water: 1:1), then place it on a heating plate, heat it on a low heat to carbonize it, and wait until the smoke exhausts After that, it shows that it has been completely carbonized. After cooling, place it in a muffle furnace, heat it at 500°C for 2 hours, take it out and cool it; add HNO dropwise 3 Solution (volume ratio of nitric acid to water: 1:1) 2.0mL was placed on electric heating and slightly boiled to dissolve, and the digestion solution was obtained;

[0028] (3) Take 1mL of rice digestion solution, adjust its pH to 10 with NaOH solution, then add 5mg of pr...

Embodiment 3

[0029] Embodiment 3: the method for the separation, enrichment and detection of Cu and Zn in food rice (set 3 repeated tests), the specific content is as follows:

[0030] (1) Put the mung bean sample in an oven at 80°C for 10 hours, and grind it to less than 80 meshes;

[0031] (2) Weigh 0.50g mung bean powder and place it in a porcelain crucible, add 1mL nitric acid solution (volume ratio of nitric acid to water: 1:1), then place it on a heating plate, heat it on a low heat to carbonize it, and wait until the smoke is exhausted After that, it shows that it has been completely carbonized. After cooling, place it in a muffle furnace, heat it at 500°C for 2 hours, take it out and cool it; The water volume ratio is 1:1), then put it on the electric heating plate and evaporate it to dryness on low heat, then put it into the muffle furnace and heat it at 500°C for 1 hour, take it out, at this time, there is white ash in the pot, indicating that it has been completely ashed, wait f...