Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of alginate oligosaccharide

A technology of fucoidan oligosaccharides and fucobiose, which is applied in the field of biomedicine, can solve the problems of inapplicable treatment of AKI and complicated pathogenesis, and achieve the effects of obvious urine concentration function, enhanced therapeutic effect, and decreased serum creatinine level

Inactive Publication Date: 2021-08-06
上海海糖生物医药科技有限公司
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current treatment of hypertensive nephropathy is mainly based on blood pressure control therapy, however, this therapy is not suitable for the treatment of AKI
Given the complex etiology of AKI, identifying a single therapy that would benefit all AKI patients is challenging

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of alginate oligosaccharide
  • Application of alginate oligosaccharide
  • Application of alginate oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 Preparation and Structure Identification of Fucobiose with Uniform Polymerization Degree

[0067] Dissolve 100 g of purchased alginate (purchased from Qingdao Mingyue Seaweed Group Co., Ltd.) in water, add fucose lyase (obtained from Ocean University of China) at a certain temperature, and centrifuge in a high-speed centrifuge after a certain period of lysis. Take the supernatant. The supernatant was purified with a gel column to remove a small amount of impurity oligosaccharides, polysaccharides and non-sugar impurities to obtain 60 g of fucobiose sodium salt with a purity of more than 95%. Gained fucobiose sodium salt is detected by high performance liquid chromatography (HPLC, 230nm), and is detected by proton nuclear magnetic spectrum ( 1 HNMR) and high-resolution mass spectrometry (HRMS-ESI) for structure identification.

[0068] HPLC: purity 99.06%, RT=13.6min (see the relevant spectrogram figure 1 );

[0069] 1 HNMR spectrum see figure 2 ;

[...

Embodiment 2

[0072] Example 2 Preparation and structure identification of fucoidan with uniform polymerization degree

[0073] Dissolve 100 g of purchased alginate in water, add fucose lyase (obtained from Ocean University of China) at a certain temperature, centrifuge in a high-speed centrifuge after a certain period of time, and take the supernatant. The supernatant was purified with a gel column to remove a small amount of impurity oligosaccharides, polysaccharides and non-sugar impurities to obtain 70 g of fucotriose sodium salt with a purity of more than 95%. Gained fucotriose sodium salt is detected by high performance liquid chromatography (HPLC, 230nm), and is detected by proton nuclear magnetic spectrum ( 1 HNMR) and high-resolution mass spectrometry (HRMS-ESI) for structure identification.

[0074] HPLC: 100% purity, RT=17.43min (see the relevant spectrum Figure 4 );

[0075] 1 HNMR spectrum see Figure 5 ;

[0076] HRMS(ESI)m / z:C 18 h 23 o 18 {(M-H) -}, the calculat...

Embodiment 3

[0078] Example 3 Preparation and Structure Identification of Fucotetraose with Uniform Polymerization Degree

[0079] Dissolve 100 g of purchased alginate in water, add fucose lyase (obtained from Ocean University of China) at a certain temperature, centrifuge in a high-speed centrifuge after a certain period of time, and take the supernatant. The supernatant was purified with a gel column to remove a small amount of impurity oligosaccharides, polysaccharides and non-sugar impurities to obtain 55 g of fucotetraose sodium salt with a purity of more than 95%. Gained fucotetraose sodium salt is detected by high performance liquid chromatography (HPLC, 230nm), and is detected by proton nuclear magnetic spectrum ( 1 HNMR) and high-resolution mass spectrometry (HRMS-ESI) for structure identification.

[0080] HPLC: purity 99.71%, RT=18.71min (see the relevant spectrogram Figure 7 );

[0081] 1 HNMR spectrum see Figure 8 ;

[0082] HRMS (ESI) m / z: C 24 h 31 o 24 {(M-H) ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of alginate oligosaccharide or pharmaceutically acceptable salt thereof in preparation of medicines for treating acute kidney injury. The alginate oligosaccharide is fucoidan, fucoidan trisaccharide or fucoidan tetrasaccharide. Researches find that the fucoidan, the fucoidan trisaccharide and the fucoidan tetrasaccharide have very obvious protection effects on an animal model with acute kidney injury induced by ischemia reperfusion (I / R), endotoxin phospholipid polysaccharide (LPS) and an antitumor drug cis-platinum. After the alginate oligosaccharide is used for treating an animal with acute kidney injury, the serum creatinine level is obviously reduced, the urine concentration function of the kidney is obviously restored, the level of renal tubular injury factors (KIM-1 and NGAL) is obviously reduced, the expression of inflammatory factors is obviously reduced, the pathological change of the kidney is obviously improved, and the treatment effect is enhanced along with the increase of the dosage. Therefore, the alginate oligosaccharide has a relatively strong effect of treating acute kidney injury.

Description

technical field [0001] The invention relates to an application of fucoidan oligosaccharide, which belongs to the technical field of biomedicine. Background technique [0002] Carbohydrates, together with nucleic acids and proteins, are called the three major living substances. Alginate is mainly found in the cell walls of kelp, sargassum and macroalgae, and is a kind of linear, unbranched, negatively charged polysaccharide compound. Alginate is a binary line composed of β-D-(1,4)-mannuronic acid (M) and α-L-(1,4)-guluronic acid (G) type block compounds. There are mainly three structural fragments in its molecule: Polymannuronate (PM), which is composed of β-D-(1,4)-mannuronic acid interconnected; α-L-(1,4)-ancient Polyguluronic acid (Polyguluronate, PG) formed by interconnecting glucuronic acid; PMG fragment formed by alternating copolymerization of M and G. [0003] The high viscosity and gel-forming properties of alginate make it widely used in food, chemical, pharmace...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7016A61K31/702A61P13/12
CPCA61K31/7016A61K31/702A61P13/12
Inventor 刘振德高河勇
Owner 上海海糖生物医药科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com