Nerve injury diagnosis developer and preparation method thereof

A technique for diagnosing imaging and nerve injury, applied in the field of nuclear medicine imaging, to achieve good stability, high accuracy, and obvious imaging effects

Active Publication Date: 2021-08-20
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular imaging agents commonly used in the detection of other diseases in the prior art cannot be directly applied to the detection of nerve damage. Therefore, it is urgent and necessary to develop a PET imaging agent for nerve damage.

Method used

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  • Nerve injury diagnosis developer and preparation method thereof
  • Nerve injury diagnosis developer and preparation method thereof
  • Nerve injury diagnosis developer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method of nerve injury diagnosis imaging agent of the present invention is as follows:

[0034] 1) Put 25ug of the precursor compound NOTA-NP41 into the EP tube, add 1ml of 0.25M NaOAc solution, mix well and transfer to the reaction tube;

[0035] 2) Use 4ml of 0.05M HCl solution to 68 GaCl 3 Rinse into the reaction tube, after rinsing 68 Ga activity is 30mCi, react at 90°C for 10min, add 10ml deionized water to quench the reaction after the reaction;

[0036] 3) Enrich the solution in the reaction tube through the C18 Plus column, and wash the C18 Plus column with 10 mL of deionized water; then rinse the C18 Plus column with 1 ml of ethanol and 10 ml of normal saline to obtain the eluate, and pass the eluate through a dry Bacterial filtration membrane and put into the product bottle to obtain imaging agent injection 68 Ga-NOTA-NP41.

[0037] Imaging Agent Injection 68 Ga-NOTA-NP41 was analyzed for HPLC purity: mobile phase A was distilled water co...

Embodiment 2

[0043] The preparation method of nerve injury diagnosis imaging agent of the present invention is as follows:

[0044] 1) Put 28ug of the precursor compound NOTA-NP41 into the EP tube, add 1ml of 0.25M NaOAc solution, mix well and transfer to the reaction tube;

[0045] 2) Use 4ml of 0.05M HCl solution to 68 GaCl 3 Rinse into the reaction tube, after rinsing 68 The Ga activity is 32mCi, react at 90°C for 10min, add 10ml of deionized water to quench the reaction after the reaction;

[0046] 3) Enrich the solution in the reaction tube through the C18 Plus column, and wash the C18 Plus column with 10mL of deionized water; then rinse the C18 Plus column with 1ml of ethanol and 10ml of normal saline to obtain the eluent, and pass the eluate through a dry Bacterial filtration membrane and put into the product bottle to obtain imaging agent injection 68 Ga-NOTA-NP41.

[0047] Imaging Agent Injection 68 Ga-NOTA-NP41 was analyzed for HPLC purity: mobile phase A was distilled wate...

Embodiment 3

[0053] The preparation method of nerve injury diagnosis imaging agent of the present invention is as follows:

[0054] 1) Put 30ug of the precursor compound NOTA-NP41 into the EP tube, add 1ml of 0.25M NaOAc solution, mix well and transfer to the reaction tube;

[0055] 2) Use 4ml of 0.05M HCl solution to 68 GaCl 3 Rinse into the reaction tube, after rinsing 68 The Ga activity is 35mCi, react at 90°C for 10min, add 10ml of deionized water to quench the reaction after the reaction;

[0056] 3) Enrich the solution in the reaction tube through the C18 Plus column, and wash the C18 Plus column with 10mL of deionized water; then rinse the C18 Plus column with 1ml of ethanol and 10ml of normal saline to obtain the eluent, and pass the eluate through a dry Bacterial filtration membrane and put into the product bottle to obtain imaging agent injection 68 Ga-NOTA-NP41.

[0057] Imaging Agent Injection 68 Ga-NOTA-NP41 was analyzed for HPLC purity: mobile phase A was distilled wate...

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Abstract

A nerve injury diagnosis developer belongs to the technical field of nuclear medicine molecular imaging, and is characterized in that polypeptide NP41 is used as a substrate, a radioactive precursor compound is synthesized through covalent linkage with a chelating group NOTA, and then a novel radioactive developer 68Ga-NOTA-NP41 is synthesized through radioactive labeling of the radioactive precursor compound and 68Ga radioactive label. The 68Ga-NOTA-NP41 developer obtained by the invention can be used for performing specific imaging on nerve extracellular matrix protein in a living body level and specificity so as to realize positioning of a nerve injury focus of an organism. After application, the kit is high in sensitivity, strong in specificity, good in stability, obvious in target organ development, high in accuracy and not prone to false positive.

Description

technical field [0001] The invention relates to the technical field of nuclear medicine imaging, in particular to an imaging agent for nerve injury diagnosis and a preparation method thereof. Background technique [0002] Nerve injury generally refers to the phenomenon of sensory disturbance, motor disturbance and nutritional disturbance in the nerve innervation area due to direct or indirect external forces (such as collision, extrusion, electric shock, etc.) on the peripheral nerve trunk and branches, often accompanied by bleeding, Fractures, soft tissue injuries, etc. Due to the relatively complicated clinical anatomy process and the characteristics of the nervous system, the recovery period of nerve injury diseases is long, the prognosis is often unsatisfactory, and the residual rate of sequelae is high. Therefore, how to detect nerve injury with high specificity, high sensitivity and high precision has extremely important clinical significance. However, there is no hi...

Claims

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Application Information

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IPC IPC(8): A61K51/08C07K7/08C07K1/107A61K103/00
CPCA61K51/08C07K7/08
Inventor胡硕周明朱泽华
OwnerXIANGYA HOSPITAL CENT SOUTH UNIV