Novel double-probiotic synergistic complex microbial inoculant and application thereof
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A technology of compound bacterial agent and double probiotics, applied in the field of microbiology, can solve the problems of repeated symptoms, abnormal pain, itching of patients, etc., and achieve the effect of preventing and treating candida vaginitis, excellent technical effect and increasing inhibition effect
Pending Publication Date: 2021-08-27
青岛诺森生物技术有限责任公司
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Because this disease will bring itching to patients, and even cause restlessness, abnormal pain and other symptoms, it will have a serious impact on the physical and mental health of patients.
The current treatment drugs mostly use fluconazole, miconazole, etc., but these drugs will cause bacteria to develop drug resistance, and the symptoms are easy to recur, so it is necessary to study more sustainable drugs with better effects
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[0028] The present invention obtains two bacterial strains by purchasing from a biological company, which are Lactobacillus fermentum Limosilactobacillus fermentum and Pediococcus lactis Pediococcus acidilactici, After activation culture, they are respectively numbered: NSL1601 and NSS0301, that is, Lactobacillus fermentum NSL1601 and Pediococcus lactis NSS0301.
[0029] Morphologically detected, see figure 1 and figure 2 , Lactobacillus fermentum NSL160 is Gram-positive, the colonies are milky white, round, opaque, slightly convex on the surface, with milk skin-like folds, and irregular edges; Arranged in a few aggregates, smooth surface, no spores. see image 3 and Figure 4 , Pediococcus lactis NSS0301 is Gram-positive, the colonies are white to milky white, round, opaque, slightly convex on the surface, wit...
Embodiment 3
[0049] Example 3: Effects of bacterial strains and synergistic compound bacterial agents on the concentration of Candida albicans in the vagina of a mouse model of candidal vaginitis
[0050] Take 40 female BALB / c mice, 8 weeks old, weighing 20-25g, and randomly divide them into 5 groups, 8 mice in each group. The 5 groups are blank control group, model group, Pediococcus lactis NSS0301 group, Lactobacillus fermentum NSL1601 group and synergistic compound bacterial agent group.
[0051] The experiment was divided into two parts: modeling and treatment. Three days before the start of the experiment, except for the blank control group, the mice in the other groups were subcutaneously injected with 100 μL of estradiol valerate to induce estrus in the mice. Except for the mice in the control group, the mice in the other groups still needed to be subcutaneously injected with 100 μL of estradiol valerate once a week on average.
[0052] On the first day of modeling, except for the ...
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Abstract
The invention discloses a novel double-probiotic synergistic complex microbial inoculant and application thereof. The double-probiotic synergistic complex microbial inoculant is prepared from lactobacillus fermentum liquid and pediococcus acidilactici liquid according to the volume ratio of 1: (1-3), the viable bacterium content of the lactobacillus fermentum liquid is not lower than 5 * 10 < 10 > CFU / mL, and the viable bacterium content of the pediococcus acidilactici liquid is not lower than 4 * 10 < 10 > CFU / mL. Experiments prove that the lactobacillus fermentum, the pediococcus acidilactici and the double-probiotic synergistic complex microbial inoculum jointly prepared from the lactobacillus fermentum and the pediococcus acidilactici have the obvious effect of inhibiting in-vivo and in-vitro tritirachium album, and then the lactobacillus fermentum, the pediococcus acidilactici and the double-probiotic synergistic complex microbial inoculum can be prepared into drugs in various forms to achieve the effect of preventing and treating candida vaginitis.
Description
technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a novel dual-probiotic synergistic compound bacterial agent and its application. Background technique [0002] Candida vaginitis is an inflammatory disease of the vagina caused by Candida infection. Candida is usually a putrefaction parasite, which can live in the skin, mucous membrane, digestive tract or other organs of normal human body, exists in the vagina and is often asymptomatic. When vaginal glycogen increases and acidity increases, or when the body's resistance decreases, it can become the cause of the disease. Long-term application of broad-spectrum antibiotics and adrenal cortex hormones can greatly increase fungal infections. Because this disease will bring itching to the patient, and even cause symptoms such as restlessness and abnormal pain, it will seriously affect the physical and mental health of the patient. The current treatment drugs mostl...
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