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A method for reducing the bioavailability of heavy metals in straw and its application

A bioavailability, heavy metal technology, applied in the field of environmental protection, can solve problems such as reducing the bioavailability of heavy metals in straw

Active Publication Date: 2022-06-21
ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, the invention of using Trichoderma filamentous fungus to reduce the bioavailability of straw heavy metals is still rarely disclosed.

Method used

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  • A method for reducing the bioavailability of heavy metals in straw and its application

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preparation example Construction

[0020] In the present invention, the Trichoderma aculeatus T-1 has been disclosed in the patent document CN104450530A, and is deposited in the General Microorganism Center of the China Microorganism Culture Collection Administration Committee, with the deposit number of CGMCC NO.9722. In the present invention, the Trichoderma aculeatus T-1 is the spore suspension of Trichoderma aculeatus T-1. In the present invention, the preparation method of the spore suspension is not particularly limited, and the preparation of the spore suspension in the specific embodiment of the present invention preferably includes the following steps:

[0021] Inoculate Trichoderma aculeatus T-1 on a PDA plate, collect spore precipitates after constant temperature culture, suspend, wash and centrifuge and resuspend the spores with sterile water to obtain the spore suspension.

[0022] In the present invention, described PDA plate medium is preferably potato medium (PDA) (L -1 ), its formula is: potat...

Embodiment 1

[0030] (1) Inoculate Trichoderma aculeatus T-1 on a PDA plate, cultivate at a constant temperature of 28°C for 3 days, wash off the spores with sterilized 0.1% (v / v) Tween 80 solution, and sterilize the three-layer After filtering with lens paper, collect the spores in a sterilized 50 mL centrifuge tube, collect the spore pellet by centrifugation, add 10 mL of 0.1% Tween 80 to resuspend the spore pellet and wash it once, centrifuge to remove the supernatant, and add 5 mL of sterile water to resuspend the spore to make Spore suspension.

[0031] Described PDA plate medium is potato medium (PDA) (L -1) , its specific preparation method is: potato 200g, glucose 20g, agar 20g, potatoes are washed, peeled and cut into small pieces, add 1L of water to boil for half an hour, filter with gauze, add 15g of glucose, fully dissolve and then pack, add 2 % agar, autoclaved at 115°C for 30min.

[0032] (2) Straw fermentation medium: The heavy metal contaminated rice straw was dried at 60°...

Embodiment 2

[0037] (1) Inoculate Trichoderma aculeatus T-1 on a PDA plate, cultivate at a constant temperature of 28°C for 5 days, wash off the spores with sterilized 0.1% (v / v) Tween 80 solution, and sterilize the three-layer After filtering with lens paper, collect the spores in a sterilized 50 mL centrifuge tube, collect the spore pellet by centrifugation, add 10 mL of 0.1% Tween 80 to resuspend the spore pellet and wash it once, centrifuge to remove the supernatant, and add 5 mL of sterile water to resuspend the spore to make Spore suspension.

[0038] Described PDA plate medium is potato medium (PDA) (L -1) , its specific preparation method is: potato 200g, glucose 20g, agar 20g, potatoes are washed, peeled and cut into small pieces, add 1L of water to boil for half an hour, filter with gauze, add 15g of glucose, fully dissolve and then pack, add 2 % agar, autoclaved at 115°C for 30min.

[0039] (2) Straw fermentation medium: The heavy metal contaminated rice straw was dried at 60°...

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Abstract

The invention relates to the technical field of environmental protection, and provides a method for reducing the bioavailability of heavy metals in plant stalks, which uses Trichoderma aculeatus T‑1 to ferment plant stalks. The present invention utilizes Trichoderma aculeatus T‑1 strain to reduce the total amount and bioavailability of heavy metals such as cadmium, copper, zinc, and manganese in plant straws, transform heavy metals from acid-weak-acid-extracted states into difficult-to-use forms for plants, and reduce plant straws. Potential release risk of heavy metals.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a method for reducing the bioavailability of heavy metals in straw and its application. Background technique [0002] In recent years, the problem of heavy metal pollution in farmland soil in my country has attracted the attention of the whole society. According to the 2014 National Soil Pollution Survey Bulletin, the excess rate of heavy metals and other pollutants in the country's cultivated soil reached 19.4%. In heavy metal-contaminated farmland soil, due to the strong bioavailability of heavy metals, they are easily absorbed into crops by crop roots, and the absorption and accumulation of heavy metals by crop straw is much higher than that of seeds. Taking rice as an example, its Most of the cadmium absorbed by the shoot vegetative organs was accumulated in the straw. The inventor's investigation in Zhejiang and Hunan in the early stage found that the aver...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12N1/14C12R1/885
CPCC12P1/02C12N1/14
Inventor 孟俊王群柴彦君张进单胜道蒋玉根
Owner ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY