New intracellular biosynthetic path detection method

Abstract

The invention discloses an intracellular new biosynthesis path detection method, which comprises: S1, preparing a sample slice, and injecting an isotope solution into the sample slice to generate a target sample slice; and s2, performing MS detection on the target sample slice to obtain a metabolic pathway of chemical substances in the sample slice. The S2 comprises the following steps: S21, performing MS detection on the target sample slice to obtain MS data corresponding to the target sample slice, and analyzing the MS data; and s22, according to an analyzing result of the MS data, obtaining a metabolic pathway of the chemical substances in the sample slices. According to the method, the ion strength value of the metabolite marked with the isotope can be standardized, the poor comparability between different samples is avoided, and effective isotope tracking is ensured, so that a complete chemical substance synthesis path is obtained.

Description

technical field [0001] The invention relates to the technical field of human biosynthesis, in particular to a method for detecting new intracellular biosynthesis pathways. Background technique [0002] With the development of science and technology, the detection of biosynthetic pathways is becoming more and more important, especially the new biosynthetic pathways in cells, which can enable researchers to have a deeper understanding of the synthesis of chemical substances to break through some difficult problems in the biological field . [0003] At present, most of the research on synthetic pathways is based on tracking isotopes, and the biosynthetic pathways are known in different compounds generated through isotopes. Therefore, it is necessary for those skilled in the art to study isotope tracking to ensure the accuracy of biosynthetic pathways. However, in the present In the prior art, the MS calculations involved in the isotope tracking process are all calculated throu...

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Problems solved by technology

[0003] At present, most of the research on synthetic pathways is based on tracking isotopes, and the biosynthetic pathways are known in different compounds generated through isotopes. Therefore, it is necessary for those skilled in the art to study isotope tracking to ensure the accuracy of biosynthetic pathways. However, in the present In the existing technology, the calculation of MS involved in the isotope tracking process is calculated through the original data, which affects its accuracy, and thus cannot guarantee effective isotope tracking to obtain a complete synthesis path of chemical substances

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