Aptamer PZ-1 targeting human highly invasive choroidal melanoma and application

Abstract

The invention relates to an aptamer PZ-1 targeting human highly invasive choroidal melanoma and application. Through property characterization of the nucleic acid aptamer PZ-1 and identification of target molecules, tumor markers of choroidal melanoma can be found, and the nucleic acid aptamer PZ-1 is used for diagnosis and treatment and targeted therapy of choroidal melanoma. The prepared nucleic acid aptamer can specifically recognize human highly invasive choroidal melanoma cells and choroidal melanoma tissues and has very high affinity; there is no immunogenicity;the molecular weight is small, and in-vitro chemical synthesis can be realized; different parts of the aptamer are easy to modify and replace. Meanwhile, the invention has the advantages of stable sequence, convenience in storage, convenience in marking and the like. When the nucleic acid aptamer is used for detecting human choroidal melanoma cells, the operation is simpler and quicker, the synthesis cost of the nucleic acid aptamer is lower than that of antibody preparation, the period is short, and the reproducibility is good.

Description

technical field [0001] The invention specifically relates to the technical field of molecular biology, in particular to a nucleic acid aptamer PZ-1 targeting human highly invasive choroidal melanoma and its application. Background technique [0002] Uveal melanoma is the most common primary malignant intraocular tumor in adults and the second most common type of melanoma after the skin. Tumors originated in the uvea, including the choroid (90%), ciliary body (6%), and iris (4%). Uveal melanoma is usually asymptomatic and has no specific tumor serum markers. Diagnosis is mainly based on ophthalmic imaging techniques, including fundus photography, fluorescein angiography, fundus autofluorescence imaging, optical coherence tomography, and ultrasound imaging, which also makes Uveal melanoma has emerged as one of the few cancers not primarily diagnosed by biopsy. [0003] Uveal melanoma is prone to metastasis, especially liver metastasis. Multiple studies have found that more ...

AI Technical Summary

Problems solved by technology

However, the use of antibodies as molecular probes often lacks ideal pharmacokinetics, and such probes are easily inactivated during chemical modification and storage

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