Preparation method of mixed bacterium enzyme liquid for efficiently hydrolyzing waste distiller's grains of white spirit

A technology of mixing bacteria and enzyme liquid, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of poor hydrolysis effect and high cost of enzymatic hydrolysis process, and achieve improved capacity, improved permeability, The effect of increasing adaptive capacity for selection

Active Publication Date: 2021-11-19
LUZHOU LAOJIAO CO LTD
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is that the hydrolysis effect of existing bacterial strains on liquor discarded grains is relatively poor, and the cost of enzymatic hydrolysis process is relatively high

Method used

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  • Preparation method of mixed bacterium enzyme liquid for efficiently hydrolyzing waste distiller's grains of white spirit

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Effect test

preparation example Construction

[0028] The preparation method of the mixed bacteria enzyme solution for efficiently hydrolyzing liquor discarded grains comprises the following steps:

[0029] a. Take samples from pit mud, waste grains, and surrounding soil of the winery, use the screening medium with liquor waste grains as the carbon source to screen and enrich, and obtain bacterial samples after separation and purification;

[0030]b. Activate the bacterial sample obtained in step a, carry out fermentation and culture, then extract it, filter it with suction, and centrifuge to obtain bacterial enzyme solution A, use bacterial enzyme solution A to enzymolyze liquor discarded grains, and screen to obtain H bacteria , the H bacteria enzyme liquid is obtained by fermentation, and the enzyme activity of the H bacteria enzyme liquid is 5.0-5.8IU / ml;

[0031] c. Sampling was taken from pit mud, distilled grains, and surrounding environmental soil of the winery, and screened and enriched with the screening medium u...

Embodiment 1

[0057] A. Disposal of grains: Rinse liquor grains with tap water, filter them with suction, and dry them to obtain dry grains;

[0058] B. Enzymolysis of discarded grains: mix the dried discarded grains with the mixed enzyme solution according to the mass volume ratio of 1:10g / ml, shake at 50°C and 140rpm at a constant temperature for 64 hours, and obtain the enzymatic sugar solution after suction filtration;

[0059] C. Inspection: The concentration of reducing sugar in the enzymatic hydrolyzed sugar solution is 50g / L, corresponding to 0.54g of reducing sugar per gram of dry waste.

Embodiment 2

[0061] A. Disposal of grains: Rinse liquor grains with tap water, filter them with suction, and dry them to obtain dry grains;

[0062] B. Enzymatic hydrolysis of discarded grains: mix the dried discarded grains with the mixed enzyme solution according to the mass volume ratio of 1:10g / ml, shake at a constant temperature of 50°C and 140rpm for 72h, and obtain the enzymatic sugar solution after suction filtration;

[0063] C. Inspection: The concentration of reducing sugar in the enzymatic hydrolyzed sugar solution is 47g / L, corresponding to 0.51g of reducing sugar per gram of dry waste.

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Abstract

The invention discloses a preparation method of mixed bacterium enzyme liquid for efficiently hydrolyzing waste distiller's grains of white spirit, and belongs to the field of recycling of waste distiller's grains of white spirit. The preparation method of the mixed bacterium enzyme liquid for efficiently hydrolyzing waste distiller's grains of white spirit comprises the following steps: conducting screening, enriching and culturing to obtain H bacterium (trichoderma reesei), L bacterium (aspergillus niger) and M bacterium (mucor) which have a synergistic effect on degradation of the waste distiller's grains, and respectively preparing an H bacterium enzyme solution, an L bacterium enzyme solution and an M bacterium enzyme solution through fermentation. Through cooperative use of the three bacterial enzyme solutions, compatibility of enzyme systems can be enhanced to achieve a mutual promotion effect, so that the hydrolysis effect of the waste distiller's grains is further improved, the use amount of the enzyme solutions is remarkably reduced, and the cost of an enzymolysis process is further reduced. The method can effectively solve the problems that the existing strain has a poor hydrolysis effect on the waste distiller's grains, and the cost of an enzymolysis process is relatively high.

Description

technical field [0001] The invention belongs to the field of reuse of liquor discarded grains, and relates to a preparation method of a mixed bacterial enzyme liquid for efficiently hydrolyzing liquor discarded grains. Background technique [0002] Liquor distiller's grains is a kind of distiller's grains. It is the husk and residual residue of grains that are poor in nutrition after repeated fermentation and distillation to extract alcohol. It has high moisture and cellulose content, but lower crude protein and crude fat than distiller's grains. The cellulose in the grain itself is a material with great development potential, which can be converted into glucose monosaccharide after hydrolysis. However, because the three macromolecular substances of cellulose, hemicellulose, and lignin constitute a dense structure—lignocellulose, which constitutes an anti-hydrolysis barrier, and the cellulose is wrapped by hemicellulose and lignin, making it difficult to is hydrolyzed. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12R1/885C12R1/685C12R1/785
CPCC12N1/14C12N1/02
Inventor 雷翔云邓波熊燕飞李勇敖灵丁海龙彭远松马卓宋川
Owner LUZHOU LAOJIAO CO LTD
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