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Construction method of ulcerative colitis animal model

A technology of ulcerative colitis and construction method, which is applied in the field of construction of animal models of ulcerative colitis, can solve the problems of long modeling cycle, low success rate, and high cost, and achieve shortening of modeling time, shortening of modeling cycle, The effect of reducing damage

Pending Publication Date: 2021-11-26
QINGDAO EASTSEA PHARMA
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  • Claims
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AI Technical Summary

Problems solved by technology

In the present invention, the novel UC animal model construction method is combined with the use of DSS to induce inflammation by reducing the immune function of mice caused by Citrobacter, which can solve the problems of easy self-healing, long modeling period, high cost and low success rate in current UC animal models The problem

Method used

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  • Construction method of ulcerative colitis animal model
  • Construction method of ulcerative colitis animal model
  • Construction method of ulcerative colitis animal model

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1 Materials and methods

[0054] 1.1 Reagents

[0055] Physiological saline; Citrobacter (ATCCS1459) freezing solution; 0.9% sodium chloride solution; LB broth medium; LB agar medium; nalidixic acid; DSS (MW4×10 4 ); HE staining kit; normal saline; ELISA kit; PCR kit; flow cytometry-related antibodies.

[0056] 1.2 Instruments and equipment

[0057] FA2004N electronic balance (analytical balance); EXL800 microplate reader; microtome; centrifuge; flow cytometer; biochemical incubator; Cell culture plates, disposable inoculation loops, etc.

[0058] 1.3 Experimental animals

[0059] Healthy Balb / c mice, 5-6 weeks old, body weight (22±2) g, female, SPF grade, Jinan Pengyue Experimental Animal Breeding Co., Ltd. The indoor temperature is 21-25°C, the relative humidity is 40%-70%, free drinking water and feeding with standard feed, artificially controlled light day and night for 12 hours, and the litter is replaced twice a week. During the experiment, the animals were ...

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Abstract

The invention relates to the field of experimental animal models, in particular to a construction method of an ulcerative colitis animal model. The construction method specifically comprises the steps that after a test animal is empty for a period of time, citrobacter and dextran sodium sulfate in a specific proportion are administered every day, and after administration is carried out for 3-5 days, the ulcerative colitis animal model is obtained. According to the novel construction method of the ulcerative colitis animal model, the mouse immune function is reduced through citrobacter, and then dextran sodium sulfate is combined for inflammation, and the problems that an existing ulcerative colitis animal model is prone to self-healing, long in modeling period, high in cost and low in success rate can be solved.

Description

technical field [0001] The invention relates to the field of experimental animal models, in particular to a method for constructing an animal model of ulcerative colitis. Background technique [0002] Ulcerative colitis (UC) is a chronic non-specific intestinal inflammatory disease characterized by continuous and diffuse inflammatory changes in the colorectal mucosa, and its global incidence is increasing. UC lesions are limited to the large intestinal mucosa and submucosa, and the clinical manifestations are diarrhea, mucus pus and blood in the stool, abdominal pain, and the course of the disease is protracted. The severity and severity of the disease vary from patient to patient, and often recur. The disease can be seen at any age. In rare cases of severe inflammation, especially those with UC that had been untreated long before diagnosis, symptoms of weight loss, fever, or perforation can develop. Extraoperative manifestations may precede the onset of gastrointestinal s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/027A01K2227/105A01K2267/0368
Inventor 崔云龙赵仕凡尚改双
Owner QINGDAO EASTSEA PHARMA
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