Biocontrol bacterium preparation and application thereof

A bio-control bacteria and preparation technology, applied in the application, biocide, fungicide and other directions, can solve the problems of the soil not reaching the expected effect, the activity retention time is short, and it is difficult to maintain the population number, etc., and achieve a significant bactericidal effect and application range. wide range of effects

Active Publication Date: 2021-12-07
HAINAN UNIVERSITY
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Problems solved by technology

[0007] However, in the process of biocontrol application of Bacillus amyloliquefaciens, many problems were found. The activity of Bacillus colonization in the soil decreased, and the soil was affected by many factors and could not achieve the expected effect. It was difficult to maintain the population in the wild environment. A series of questions such as the number of
Therefore, when Bacillus amyloliquefaciens is used alone as a biocontrol agent, the activity retention time is short in actual application, which is not conducive to large-scale industrial production and application.

Method used

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  • Biocontrol bacterium preparation and application thereof
  • Biocontrol bacterium preparation and application thereof
  • Biocontrol bacterium preparation and application thereof

Examples

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preparation example Construction

[0047] The preparation method of biocontrol bacteria preparation of the present invention comprises the steps:

[0048] (1) adopting LB liquid culture medium to prepare Bacillus Velez HAB-2 bacterium liquid;

[0049] (2) Combine Bacillus veleisi HAB-2 bacterial liquid with appropriate amount of natamycin, zein, sorbic acid, glycerol, Tween and Nongru 500 # Mixing is to prepare the biocontrol bacteria preparation.

[0050] In step (1), Bacillus Velez HAB-2 bacterium liquid adopts LB liquid medium to carry out shaker culture, and the preparation method of LB liquid medium is: get tryptone 10g, yeast extract 5g, NaCl10g, after dissolving, Adjust the pH to 7.0 with 5mol / L NaOH, make up to 1L with deionized water, and steam sterilize at 15psi high pressure for 20min. Bacillus Velez HAB-2 was shaken and cultured in LB liquid medium, 28°C, 180-200r / min constant temperature shaking culture for 24h, so that the total number of viable bacteria of Bacillus Velez HAB-2 was ≥5×10 7 cfu / ...

Embodiment 2

[0059] The biocontrol bacteria preparation of this embodiment, calculated by 1000mL, contains 700mL total number of viable bacteria ≥ 5 × 10 7 cfu / mL culture of Bacillus velei HAB-2, 90g natamycin (calculated as pure natamycin), 11g zein (calculated as pure zein), 45g sorbic acid (calculated as pure sorbic acid), 30g of glycerin, 25g of Tween 20, 35g of agricultural milk 500 # , the balance is water;

[0060] The preparation method of the biocontrol bacteria preparation of the present embodiment may further comprise the steps:

[0061] (1) adopt LB liquid culture medium to prepare Bacillus velei HAB-2 bacterium liquid,

[0062] Dissolve 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl, adjust the pH to 7.0 with 5 mol / L NaOH, adjust the volume to 1 L with deionized water, and steam sterilize at 15 psi for 20 minutes. Bacillus Velez HAB-2 was cultured in LB liquid medium with shaking, 28°C, 180-200r / min constant temperature shaking culture for 24h, so that the total n...

Embodiment 3

[0065] The biocontrol bacteria preparation of this embodiment, calculated by 1000mL, contains 700mL total number of viable bacteria ≥ 5 × 10 7 cfu / mL culture of Bacillus velei HAB-2, 100g natamycin (calculated as pure natamycin), 12g zein (calculated as pure zein), 50g sorbic acid (calculated as pure sorbic acid), 30g of glycerin, 25g of Tween 20, 35g of agricultural milk 500 # , the balance is water;

[0066] The preparation method of the biocontrol bacteria preparation of the present embodiment may further comprise the steps:

[0067] (1) adopt LB liquid culture medium to prepare Bacillus velei HAB-2 bacterium liquid,

[0068] Dissolve 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl, adjust the pH to 7.0 with 5 mol / L NaOH, adjust the volume to 1 L with deionized water, and steam sterilize at 15 psi for 20 minutes. Bacillus Velez HAB-2 was cultured in LB liquid medium with shaking, 28°C, 180-200r / min constant temperature shaking culture for 24h, so that the total ...

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Abstract

The invention discloses a biocontrol bacterium preparation, which is liquid and comprises the following active ingredients: bacillus velezensis HAB-2, natamycin, sorbic acid, zein, glycerol, tween-20 and pesticide emulsifier 500 #, wherein the total viable count of the bacillus velezensis HAB-2 is greater than or equal to 5 * 10 < 7 > cfu/mL, the concentration of the natamycin is 8-10 wt%, the concentration of the zein is 1.0-1.2 wt%, and the concentration of the sorbic acid is 4-5 wt%. The effective components of the biocontrol bacterium preparation are integrated with active components with different action mechanisms, the control effect on plant fungal diseases is better under a synergistic effect, and all the components are green, environment-friendly and non-toxic active components with pesticide effects.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a biocontrol bacteria preparation containing Bacillus Velez HAB-2, a preparation method and application thereof. Background technique [0002] Plant fungal diseases include rubber tree anthracnose, tomato gray mold, watermelon blight, rice blast, mango anthracnose, yellow skin anthracnose, and banana leaf spot. Medication will inevitably lead to problems such as pesticide residues, pathogenic bacteria resistance and environmental pollution. According to relevant reports from China Pesticide Information Network, as of the end of May 2020, among the 41,614 pesticides registered within the effective period in China, only 47 microbial pesticides were registered, and the actual number of registered products was 434, accounting for only 2.9% of the total pesticide products. It can be seen that the research and development potential of biological pesticides is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01N43/90A01N37/06A01N63/50A01P3/00C12R1/07
CPCC12N1/20A01N63/22A01N43/90A01N37/06A01N63/50
Inventor 刘文波秦春秀缪卫国
Owner HAINAN UNIVERSITY
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