Multi-specific single chain antibody and application thereof
A single-chain antibody and multi-specific technology, applied to specific peptides, peptides containing affinity tags, peptides containing His tags, etc., can solve the problems of affecting T cell activity, T cell damage, cumbersome process, etc., and achieve enhanced Effects of bonding force, damage reduction, and simplified separation steps
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0049] 1. Expression and purification of anti-CD3CD28 bispecific single chain antibody
[0050] The sequence of the anti-CD3CD28 bispecific single-chain antibody molecule is shown in the table below. The relevant sequence was synthesized by nucleotides, and then cloned into the pcDNA3.1 vector, expressed by transfection into the 293T cell line, and the supernatant was collected and layered with nickel The expressed antibody was purified by column analysis, and the results after purification can be found in figure 1 .
[0051] according to figure 1 The results of expression and purification indicated that the anti-CD3CD28 bispecific single-chain antibody had been successfully obtained, and the molecular weight was also correct.
[0052]
[0053]
[0054]
[0055]
[0056] 2. Whole blood separation experiment
[0057] The expressed and purified anti-CD3CD28 bispecific single-chain antibody molecule was biotin-labeled with a biotin labeling kit, and labeled and pur...
Embodiment 2
[0064] The anti-CD3CD28 bispecific single chain antibody was expressed and purified by the method shown in Example 1.
[0065] The expressed and purified anti-CD3CD28 bispecific single-chain antibody molecules were biotin-labeled with a biotin labeling kit, and labeled and purified according to the manufacturer's instructions, so as to prepare for subsequent whole blood separation experiments to verify its feasibility. At the same time, a streptavidin protein-coated cellulose membrane is prepared, and the coated cellulose membrane is placed in a cell culture bag that has connected infusion tubes in the upper and lower directions.
[0066] Take 50mL of whole blood, add 100mL of normal saline to dilute, then add 50mg of biotin-labeled anti-CD3CD28 bispecific single-chain antibody molecules, mix well and incubate at room temperature for 5 minutes, then use gravity infusion to flow through the filled streptavidin-coated Cell culture bag with a cellulose membrane, unbound cells flo...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com